Biotransformation of ginseng extract to cytotoxic compound K and ginsenoside Rh2 by human intestinal bacteria

Natural Product Sciences

Volumn 10, Issue 6, 2004, Pages 347-352

  Bae, Eun Ah ^a   Choo, Min Kyung ^a   Lee, Young Churl ^b   Kim, Dong Hyun ^a  

^a Kyung Hee University   (South Korea)

^b Korea Food Research Institute   (South Korea)

Author keywords

Compound K; Cytotoxicity; Ginseng; Ginsenoside Rh 2; Intestinal bacteria; Transformation

Indexed keywords

COMPOUND K; CYTOTOXIC AGENT; DOXORUBICIN; DRUG METABOLITE; GINSENG EXTRACT; GINSENOSIDE; GINSENOSIDE RB 1; GINSENOSIDE RB 2; GINSENOSIDE RC; GINSENOSIDE RD; GINSENOSIDE RG 3; GINSENOSIDE RH 2; PROBIOTIC AGENT; SAPONIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTINEOPLASTIC ACTIVITY; ARTICLE; BACTEROIDES; CELL STRAIN HEPG2; CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG METABOLISM; DRUG TRANSFORMATION; FECES; GINSENG; HELA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; INTESTINE FLORA; LACTIC ACID BACTERIUM; LEUKEMIA P 388; LUNG CARCINOMA; MOUSE; NONHUMAN; THIN LAYER CHROMATOGRAPHY; TUMOR CELL LINE;

EID: 14744280028     PISSN: 12263907     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Akao, T., Kanaoka, M., Kobashi, K., Appearance of compound K, a major metbolite of ginsenoside Rb1 by intestinal bacteria, in rat plasma after oral aministrationmeasruement of compound K by enzyme immunoassay. Biol. Pharm. Bull., 21, 245-249 (1998a).
2. Akao, T., Kida, H., Kanaoka, M., Hattori, M., Kobashi, K., Intestinal bacterial hydrolysis is required for the appearance of compound K in rat plasma after oral administration of ginsenoside Rb1 from Panax ginseng. J. Pharm. Pharmacol., 50, 1155-1160 (1998b).
3. Bae, E.A., Park, S.Y., Kim, D.H., Constitutive β-glucosidases hydrolyzing ginsenoside Rb1 and Rb2 from human intestinal bacteria. Biol. Pharm. Bull., 23, 1481-1485 (2000)
4. Bae, E.A., Man, M.J., Choo, M.K., Park, S.Y., Kim, D.H., Metabolism of 20(S)- and 20(R)-ginsenoside Rg3 by human intestinal bacteria and its relation to in vitro biological activities. Biol. Pharm. Bull., 25, 58-63 (2002).
5. Carmichael, J., DeGreff, W.G., Gazdar, A.F., Minna, J.D., Mitchell, J.B., Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chmosensitivity testing. Cancer Res., 47, 936-940 (1987).
6. Han, B.H., Park, M.H., Han, Y.N., Woo, L.K., Sankawa, U., Yahara, S., Tanaka, O., Degradation of ginseng saponins under mild acidic conditions. Planta Med., 44, 146-149 (1982).
7. Han, B.H., Park, M.H., Han, Y.N., Woo, L.K., Sankawa, U., Yahara, S., Tanaka, O., Degradation of ginseng saponins under mild acidic conditions. Planta Med., 44, 146-149 (1982).
8. Hasegawa, H., Sung, J.H., Benno, Y., Role of human intestinal Prevotella oris in hydrolyzing Ginseng saponis. Planta Med., 63, 436-440 (1997).
9. Holdeman LV, Kelley RW, and Moore WEC. Genus Bacteroides, In Krieg N.R., and J.G. Holt (ed.) Bergeys manual of systemic bacteriology. Williams and Wilkins, Baltimore, pp 602-631 (1984).
10. Kanaoka, M, Kato, H, Shimada, E, Yano, S., Studies on the enzyme immunoassay of bioactive constituents contained in oriental medicinal drugs. VI. Enzyme immunoassay of ginesenoside Rb1 form Panax ginseng. Chem. Pharm. Bull., 40, 314-317 (1992).
11. Kanaoka, M., Akao, T., Kobashi, K., Metabolism of ginseng saponins, ginsenosides, by human intestinal bacteria. J. Tradit. Med., 11, 241-245 (1994).
12. Karikura M., Miyaze T., Tanizawa H., Taniyzma T., Takino Y., Studies on absorption, distribution, excretion and metabolism of ginseng saponins. VII. Comparison of the decomposition modes of ginsenoside Rb1 and Rb2 in the digestive tract of rats. Chem. Pharm. Bull., 39, 2357-2361 (1991).
13. Kitagawa, I., Yoshikawa, M., Yoshihara, M., Hayashi, T., Taniyama, T., Chemical studies on crude drug precession. I. On the constituents of ginseng radix rubura (I). Yakugaku Zasshi, 103, 612-622 (1983).
14. Lee S.J., Sung J.H., Lee S.J., Moon C.K., Lee B.H., Antitumor activity of a novel ginseng saponin metabolite in human pulmonary adenocarcinoma cells resistant to cisplatin. Cancer Lett., 144, 39-43 (1999).
15. Mochizuki et al. Mochizuki M, Yoo CY, Matsuzawa K, Sato K, Saiki I, Tono-oka S, Samukawa K, Azuma I. Inhibitory effect of tumor metastasis in mice by saponins, ginsenoside Rb2, 20(R)- and 20(S)-ginsenoside Rg3, of Red ginseng. Biol. Pharm. Bull., 18, 1197-1202 (1995).
16. Sato K., Mochizuki M., Saiki I., Yoo Y.C., Samukawa K., Azuma I., Inhibition of tumor angiogenesis and metastasis by a saponin of Panax ginseng-ginsenoside Rb2. Biol. Pharm. Bull., 17, 635-639 (1994).
17. Shibata, S., Fujita, M., Itokawa, H., Tanaka, O., Ishii, T., Panaxadiol, a sapongenin of ginseng roots (1). Chem. Pharm. Bull., 11, 759-764 (1963).
18. Tanaka N., Tanaka O., Shibata S., Chemical studies on the oriental plant drugs. XXVIII. Saponins and sapogenins of ginseng; Stereochemistry of sapogenin of ginsenoside Rb1, Rb2 and Rc. Chem. Pharm. Bull., 20, 1212-1216 (1972).
19. Wakabayashi C., Hasegawa H., Murata J., Saiki I., In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration. Oncol. Res., 9, 411-417 (1998).
20. Wu J.Y., Gardner B.H., Murphy CL, Seals J.R., Kensil C.R., Recchia J., Beltz G.A., Newman G.W., Newman M.J., Saponin adjuvant enhancement of antigen-specific immune responses to an experimental HIV-1 vaccine. J. Immunol., 148, 1519-1525 (1992).