Determination of the molecular weight distribution of the PEGylated bovine hemoglobin (PEG-bHb)

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology

Volumn 33, Issue 1, 2005, Pages 13-25

  Li, Xianzhong ^a   Zhang, Xiaowei ^a   Liu, Qian ^a,b  

^a Beijing Kaizheng Biotech Dev Ltd   (China)

^b PEKING UNION MEDICAL COLLEGE   (China)

Author keywords

Distribution of molecular weight; Light scattering; Molecular weight; PEG bHb; SE HPLC

Indexed keywords

DIMERS; DISSOCIATION; HEMOGLOBIN; LIGHT SCATTERING; POLYPEPTIDES; PROTEINS; REFRACTIVE INDEX;

MOLAR RATIOS; PEGYLATED HEMOGLOBIN (PEG-BHB); PROTEIN MOIETY; UV ABSORBANCE;

MOLECULAR WEIGHT DISTRIBUTION;

BOVINE SERUM ALBUMIN; HEMOGLOBIN DERIVATIVE; MACROGOL; PEGYLATED BOVINE HEMOGLOBIN; POLYPEPTIDE; TETRAMER; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CALIBRATION; CONTROLLED STUDY; DISSOCIATION; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIGHT SCATTERING; MOLECULAR WEIGHT; NONHUMAN; PROTEIN AGGREGATION; REFRACTION INDEX; RELIABILITY; ULTRAVIOLET SPECTROSCOPY;

ANIMALS; CATTLE; HEMOGLOBINS; MOLECULAR WEIGHT; POLYETHYLENE GLYCOLS; SPECTROPHOTOMETRY, ULTRAVIOLET;

BOVINAE;

EID: 14644435136     PISSN: 10731199     EISSN: None     Source Type: Journal    
DOI: 10.1081/BIO-200046639     Document Type: Article

References (27)

1. Feola, M., Simoni, J., Canizarro, P.C. (1998). Toxicity of polymerized hemoglobin solutions. Surgery. Gynecology & Obstetrics. 166: 211-222.
2. Shoemaker, S.A., Gerber, M.J. (1994). Initial clinical experience with a rationally designed, genetically engineered recombinant human hemoglobin. Art. Cells, Blood Subs., and Immobil. Biotech. 22(3): 457-465.
3. Gulati, A., Barve, A., Sen, A.P. (1998). Pharmacology of hemoglobin therapeutics. J. Lab. Clin. Med. 133(2): 112-119.
4. Haney, C.R., Buehler Pharm, P.W., Gulati, A. (2000). Purification and chemical modifications of hemoglobin in developing hemoglobin based oxygen carriers. Advan. Drug. Deliver. Review 40: 153-169.
5. Conover, C.D., Linberg, R. Shun, K.L., Shorr, R.G. (1999). The ability of polyethylene glycol conjugated bovine hemoglobin (PEG-bHb) to adequately deliver oxygen in both exchange transfusion and top-load rat models. Art. Cells, Blood Subs., and Immobil. Biotech. 27(2): 93-107.
6. Vandegriff, K.D., McCarthy, M., Rohlfs, R., Winslow, R.M. (1997). Colloid osmotic properties of modified hemoglobins chemically cross-linked versus polyethylene glycol surface-conjugated. Biophs. Chemistry. 69: 23-30.
7. Fairman, R., Fendrson, W., Hail, M.E., Wu, Y.L. (1999). Molecular weight of CTLA-4 and CD80 by sedimentation. Anal. Biochem. 270: 270-286.
8. Chiancone, E., Gilbert, G.A. (1965). Dissociation of hemoglobin into subunits. I. Oxyhemoglobin: effect of acetic acid. J. Biol. Chem. 240: 3866-3867.
9. Smith, R.D., Loo, A. (1990). New developments in biochemical mass spectrometry: electrospray ionization. Anal. Chem. 62: 882-893.
10. Kunitani, M., Dollinger, G., Johnson, D., Kresin, L. (1991). On-line characterization of polyethylene glycol-modified proteins. J. Chromatogr. 588: 125-137.
11. Kunitani, M., Kresin, L. (1993). High-performance liquid chromatographic analysis of carbohydrate mass composition glyciproteins. J. Chromatogr. 632: 19-28.
12. Maezawa, S., Takagi, T. (1983). Monitoring of the elution from a high-performance gel chromatography column by a spectrophotometer, a low-anger light scattering photometer and a precision differential refractometer as a versatile way to determine protein molecular weight. J. Chromatogr. 280: 124-130.
13. Takagi, T. (1990). Application of low-angle laser light scattering detection in the field of biochemistry. J. Chromatogr. 506: 409-416.
14. Dollinger, G., Cunico, B., Kunitani, M., Johson, D., Jones, R. (1992). Practical on-line determination of biopolymer molecular weight by high performance liquid chromatography with classical light-scattering detection. J. Chromatogr. 592: 215-228.
15. Oliva, A., Llabers, M., Farina, J.B. (2001). Comparative study of protein molecular weights by size-exclusion chromatography and laser-light scattering. J. Pharm: Biomed. Anal. 25: 833-841.
16. Arakawa, T., Langley, K.E., Kameyama, K., Takagi, T. (1992). Molecular weight of glycosylated and nonglycosylated forms of recombinant human stem cell factor determined by low-angle laser light scattering. Anal. Biochem. 230: 53-57.
17. Kato, A., Kameyama, K., Takagi, T. (1992). Molecular weight determination and compositional analysis of dextran-protein conjugates using low-angle laser light scattering technique combined with high-performance gel chromatography. Biochim. Biophys. Acta. 1159: 22-28.
18. Arakawa, T., Wen, J. (2001). Determination of carbohydrate contents from excess light scattering. J. Anal. Biochem. 299: 158-161.
19. Wen, J., Arakawa, T., Philo, J.S. (1996). Size-exclusion chromatography with on-line light-scattering, absorbance, and refractive index detectors for studying proteins and their interaction. Anal. Biochem. 240: 155-166.
20. Kendrik, B.S., Kerwin, B.A., Chang, B.S., Philo, J.S. (2001). Online ssize-exclusion high-performance liquid chromatography light scattering and differential refractometry methods to determine degree of polymer conjugation to proteins and protein-protein or protein-lignad association states. Anal. Biochem. 299: 134-136.
21. Philo, J., Talvenheomo, J., Wen, J. (1994). Interactions of neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), and the NT-3.BDNF heterodimer with the extracellular domains of the TrkB and TrkC receptors. J. Boi. Chem. 269: 27840-27846.
22. Wyatt, P.J. (1993). Light scattering and absolute characterization of macromolecules. Anal. Chem. Acta. 272: 1-40.
23. Franck, Z. (1996). The multi-hemoglobin system of the hydrothermal vent tube worm Riftia pachyptila. J. Bio. Chem. 271: 8869-8874.
24. Everse, J. (1995). Methods of Enzyme, 231.
25. Babara, S., Cristinna, D., Derek, F. (1993). Analysis and purification of monometh-oxy-polyethylene glycol by vesicle and gel permeation chromatography. J. Chromatogr. 641: 71-179.
26. Yamaguchi, T., Adachi, K. (2002). Hemoglobin equilibrium analysis by the multiangle laser light-scattering method. Biotech. Biophys. Res. Communi. 290: 1382-1387.
27. Lunelli, L., Zuliani, P., Baldini, G. (1994). Evidence of hemoglobin dissociation. Biopolymers. 4: 747-757.