HetR-dependent and -independent expression of heterocyst-related genes in an Anabaena strain overproducing the NtcA transcription factor

Journal of Bacteriology

Volumn 187, Issue 6, 2005, Pages 1985-1991

  Olmedo Verd, Elvira ^a   Flores, Enrique ^a   Herrero, Antonia ^a   Muro Pastor, Alicia M ^a,b  

^a UNIVERSITY OF SEVILLE   (Spain)

^b Universidad de Sevilla CSIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; NITROGEN; TRANSCRIPTION FACTOR;

ANABAENA; ARTICLE; BACTERIAL STRAIN; GENE EXPRESSION; GENE OVEREXPRESSION; GENETIC VARIABILITY; NONHUMAN; OPERON; PRIORITY JOURNAL;

ANABAENA; ATP-BINDING CASSETTE TRANSPORTERS; BACTERIAL PROTEINS; CYCLOOXYGENASE 2; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; NITROGEN; NITROGENASE; OPERON; OXIDOREDUCTASES; PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

ANABAENA; ANABAENA SP.;

EID: 14644427750     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.187.6.1985-1991.2005     Document Type: Article

References (46)

1. Amann, E., B. Ochs, and K. J. Abel. 1988. Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene 69:301-315.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 2003. Current protocols in molecular biology. Greene Publishing/Wiley-Interscience, New York, N.Y.
3. Black, T. A., Y. Cai, and C. P. Wolk. 1993. Spatial expression and autoregulation of hetR. a gene involved in the control of heterocyst development in Anabaena. Mol. Microbiol. 9:77-84.
4. Brusca, J. S., C. J. Chastain, and J. W. Golden. 1990. Expression of the Anabaena sp. strain PCC 7120 xisA gene from a heterologous promoter results in excision of the nifD element. J. Bacteriol. 172:3925-3931.
5. Buikema, W. J., and R. Haselkorn. 1991. Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena 7120. Genes Dev. 5:321-330.
6. Cai, Y., and C. P. Wolk. 1990. Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences. J. Bacteriol. 172:3138-3145.
7. Chastain, C. J., J. S. Brusca, T. S. Ramasubramanian, T.-F. Wei, and J. W. Golden. 1990. A sequence-specific DNA-binding factor (VF1) from Anabaena sp. strain PCC 7120 vegetative cells binds to three adjacent sites in the xisA upstream region. J. Bacteriol. 172:5044-5051.
8. Elhai, J., A. Vepritskiy, A. M. Muro-Pastor, E. Flores, and C. P. Wolk. 1997. Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. J. Bacteriol. 179:1998-2005.
9. Elhai, J., and C. P. Wolk. 1988. Conjugal transfer of DNA to cyanobacteria. Methods Enzymol. 167:747-754.
10. Elhai, J., and C. P. Wolk. 1990. Developmental regulation and spatial pattern of expression of the structural genes for nitrogenase in the cyanobacterium Anabaena. EMBO J. 9:3379-3388.
11. Elhai, J., and C. P. Wolk. 1988. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68:119-138.
12. Fiedler, G., M. Arnold, S. Hannus, and I. Maldener. 1998. The DevBCA exporter is essential for envelope formation in heterocysts of the cyanobacterium Anabaena sp. strain PCC 7120. Mol. Microbiol. 27:1193-1202.
13. Fiedler, G, A. M. Muro-Pastor, E. Flores, and I. Maldener. 2001. NtcA-dependent expression of the devBCA operon, encoding a heterocyst-specific ATP-binding cassette transporter in Anabaena spp. J. Bacteriol. 183:3795-3799.
14. Flores, E., and A. Herrero. 1994. Assimilatory nitrogen metabolism and its regulation, p. 487-517. In D. A. Bryant (ed.), The molecular biology of cyanobacteria. Kluwer Academic Publishers, Dordrecht, The Netherlands.
15. Frías, J. E., E. Flores, and A. Herrero. 1997. Nitrate assimilation gene cluster from the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol. 179:477-486.
16. Frías, J. E., E. Flores, and A. Herrero. 1994. Requirement of the regulatory protein NtcA for the expression of nitrogen assimilation and heterocyst development genes in the cyanobacterium Anabaena sp. PCC 7120. Mol. Microbiol. 14:823-832.
17. Golden, J. W., C. D. Carrasco, M. E. Mulligan, G. J. Schneider, and R. Haselkorn. 1988. Deletion of a 55-kilobase-pair DNA element from the chromosome during heterocyst differentiation of Anabaena sp. strain PCC 7120. J. Bacteriol. 170:5034-5041.
18. Golden, J. W., S. J. Robinson, and R. Haselkorn. 1985. Rearrangement of nitrogen fixation genes during heterocyst differentiation in the cyanobacterium Anabaena. Nature 314:419-423.
19. Golden, J. W., L. L. Whorff, and D. R. Wiest. 1991. Independent regulation of nifHDK operon transcription and DNA rearrangement during heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol. 173:7098-7105.
20. Golden, J. W., and H. S. Yoon. 2003. Heterocyst development in Anabaena. Curr. Opin. Microbiol. 6:557-563.
21. Hebbar, P. B., and S. E. Curtis. 2000. Characterization of devH, a gene encoding a putative DNA binding protein required for heterocyst function in Anabaena sp. strain PCC 7120. J. Bacteriol. 182:3572-3581.
22. Herrero, A., A. M. Muro-Pastor, and E. Flores. 2001. Nitrogen control in cyanobacteria. J. Bacteriol. 183:411-425.
23. Herrero, A., A. M. Muro-Pastor, A. Valladares, and E. Flores. 2004. Cellular differentiation and the NtcA transcription factor in filamentous cyanobacteria. FEMS Microbiol. Rev. 28:469-487.
24. Huang, X., Y. Dong, and J. Zhao. 2004. HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS. Proc. Natl. Acad. Sci. USA 101:4848-4853.
25. Li, J. H., S. Laurent, V. Ronde, S. Bédu, and C.-C. Zhang. 2003. An increase in the level of 2-oxoglutarate promotes heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120. Microbiology 149:3257-3263.
26. Luque, I., E. Flores, and A. Herrero. 1994. Molecular mechanism for the operation of nitrogen control in cyanobacteria. EMBO J. 13:2862-2869.
27. Luque, I., M. F. Vázquez-Bermúdez, J. Paz-Yepes, E. Flores, and A. Herrero. 2004. In vivo activity of the nitrogen control transcription factor NtcA is subjected to metabolic regulation in Synechococcus sp. strain PCC 7942. FEMS Microbiol. Lett. 236:47-52.
28. Mackinney, G. 1941. Absorption of light by chlorophyll solutions. J. Biol. Chem. 140:109-112.
29. Markwell, M. A., S. M. Haas, L. L. Bieber, and N. E. Tolbert. 1978. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem. 87:206-210.
30. Marsh, J. L., M. Erfle, and E. J. Wykes. 1984. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481-485.
31. Muro-Pastor, A. M., A. Valladares, E. Flores, and A. Herrero. 1999. The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development. J. Bacteriol. 181:6664-6669.
32. Muro-Pastor, A. M., A. Valladares, E. Flores, and A. Herrero. 2002. Mutual dependence of the expression of the cell differentiation regulatory protein HetR and the global nitrogen regulator NtcA during heterocyst development. Mol. Microbiol. 44:1377-1385.
33. Muro-Pastor, M. I., J. C. Reyes, and F. J. Florencio. 2001. Cyanobacteria perceive nitrogen status by sensing intracellular 2-oxoglutarate levels. J. Biol. Chem. 276:38320-38328.
34. Ramasubramanian, T. S., T.-F. Wei, and J. W. Golden. 1994. Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell- and heterocyst-specific genes. J. Bacteriol. 176:1214-1223.
35. Ramasubramanian, T. S., T.-F. Wei, A. K. Oldham, and J. W. Golden. 1996. Transcription of the Anabaena sp. strain PCC 7120 ntcA gene: multiple transcripts and NtcA binding. J. Bacteriol. 178:922-926.
36. Rippka, R., J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier. 1979. Generic assignments, strain stories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111:1-61.
37. Studier, F. W., A. H. Rosenberg, J. J. Dunn, and J. W. Dubendorff. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
38. Tanigawa, R., M. Shirokane, S.-I. Maeda, T. Omata, K. Tanaka, and H. Takahashi. 2002. Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro. Proc. Natl. Acad. Sci. USA 99:4251-4255.
39. Valladares, A., A. Herrero, D. Pils, G. Schmetterer, and E. Flores. 2003. Cytochrome c oxidase genes required for nitrogenase activity and diazotrophic growth in Anabaena sp. PCC 7120. Mol. Microbiol. 47:1239-1249.
40. Vázquez-Bermúdez, M. F., A. Herrero, and E. Flores. 2002. 2-Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter. FEBS Lett. 512:71-74.
41. Vázquez-Bermúdez, M. F., A. Herrero, and E. Flores. 2003. Carbon supply and 2-oxoglutarate effects on expression of nitrate reductase and nitrogen-regulated genes in Synechococcus sp. strain PCC 7942. FEMS Microbiol. Lett. 221:155-159.
42. Vega-Palas, M. A., E. Flores, and A. Herrero. 1992. NtcA, a global nitrogen regulator from the cyanobacterium Synechococcus that belongs to the Crp family of bacterial regulators. Mol. Microbiol. 6:1853-1859.
43. Vioque, A. 1997. The RNase P RNA from cyanobacteria: short tandemly repeated repetitive (STRR) sequences are present within the RNase P RNA gene in heterocyst-forming cyanobacteria. Nucleic Acids Res. 25:3471-3477.
44. Wei, T.-F., T. S. Ramasubramanian, and J. W. Golden. 1994. Anabaena sp. strain PCC 7120 ntcA gene required for growth on nitrate and heterocyst development. J. Bacteriol. 176:4473-4482.
45. Wolk, C. P. 2000. Heterocyst formation in Anabaena, p. 83-104. In Y. V. Brun and L. J. Shimkets (ed.), Prokaryotic development. American Society for Microbiology, Washington, D.C.
46. Zhou, R., X. Wei, N. Jiang, H. Li, Y. Dong, K.-L. Hsi, and J. Zhao. 1998. Evidence that HetR protein is an unusual serine-type protease. Proc. Natl. Acad. Sci. USA 95:4959-4963.