The conserved histidine 295 does not contribute to proton cotransport by the glutamate transporter EAAC1

Biochemistry

Volumn 44, Issue 9, 2005, Pages 3466-3476

  Tao, Zhen ^a   Grewer, Christof ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; PH; PHYSIOLOGY; POSITIVE IONS; SUBSTRATES; TRANSPORT PROPERTIES;

EXCITATORY AMINO ACID CARRIER (EAAC); GLUTAMATE AFFINITY; HISTIDINE; TRANSMEMBRANE DOMAINS;

BIOLOGICAL MEMBRANES;

EXCITATORY AMINO ACID TRANSPORTER; GLUTAMATE TRANSPORTER; GLUTAMINE; HISTIDINE; PROTON; SODIUM ION;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ELECTROPHYSIOLOGY; EXTRACELLULAR SPACE; KINETICS; MEMBRANE POTENTIAL; NONHUMAN; OXIDATION; PH; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTON TRANSPORT; RAT; SITE DIRECTED MUTAGENESIS; TRANSPORT KINETICS; WILD TYPE;

AMINO ACID SUBSTITUTION; AMINO ACID TRANSPORT SYSTEM X-AG; ANIMALS; BIOLOGICAL TRANSPORT, ACTIVE; CELL LINE; CONSERVED SEQUENCE; CYSTEINE; EXCITATORY AMINO ACID TRANSPORTER 3; GLUTAMATE PLASMA MEMBRANE TRANSPORT PROTEINS; GLUTAMIC ACID; GLUTAMINE; HISTIDINE; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; LYSINE; MODELS, CHEMICAL; PATCH-CLAMP TECHNIQUES; PROTONS; RATS; SYMPORTERS; TRANSFECTION;

EID: 14644419554     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi047812i     Document Type: Article

References (32)

1. Kanner, B. I., and Sharon, I. (1978) Active transport of L-glutamate by membrane vesicles isolated from rat brain, Biochemistry 17, 3949-3953.
2. Kanner, B. I., and Bendahan, A. (1982) Binding order of substrates to the sodium and potassium ion coupled L-glutamic acid transporter from rat brain, Biochemistry 21, 6327-6330.
3. Erecinska, M., Wantorsky, D., and Wilson, D. F. (1983) Aspartate transport in synaptosomes from rat brain, J. Biol. Chem. 258, 9069-9077.
4. Zerangue, N., and Kavanaugh, M. P. (1996) Interaction of L-cysteine with a human excitatory amino acid transporter, J. Physiol. 493, 419-423.
5. Zerangue, N., and Kavanaugh, M. P. (1996) Flux coupling in a neuronal glutamate transporter, Nature 383, 634-637.
6. Watzke, N., Rauen, T., Bamberg, E., and Grewer, C. (2000) On the mechanism of proton transport by the neuronal excitatory amino acid carrier 1, J. Gen. Physiol. 116, 609-622.
7. Seal, R. P., Shigeri, Y., Eliasof, S., Leighton, B. H., and Amara, S. G. (2001) Sulfhydryl modification of V449C in the glutamate transporter EAAT1 abolishes substrate transport but not the substrate-gated anion conductance, Proc. Natl. Acad. Sci. U.S.A. 98, 15324-15329.
8. Grewer, C., Watzke, N., Rauen, T., and Bicho, A. (2003) Is the glutamate residue Glu-373 the proton acceptor of the excitatory amino acid carrier 1?, J. Biol. Chem. 278, 2585-2592.
9. +-dependent neutral amino acid transporter, J. Biol. Chem. 271, 14883-14890.
10. Broer, A., Brookes, N., Ganapathy, V., Dimmer, K. S., Wagner, C. A., Lang, F., and Broer, S. (1999) The astroglial ASCT2 amino acid transporter as a mediator of glutamine efflux, J. Neurochem. 73, 2184-2194.
11. Padan, E., Sarkar, H. K., Viitanen, P. V., Poonian, M. S., and Kaback, H. R. (1985) Site-specific mutagenesis of histidine residues in the lac permease of Escherichia coli, Proc. Natl. Acad. Sci. U.S.A. 82, 6765-6768.
12. Abramson, J., Smirnova, I., Kasho, V., Verner, G., Kaback, H. R., and Iwata, S. (2003) Structure and mechanism of the lactose permease of Escherichia coli, Science 301, 610-615.
13. + exchangers, Biochem. J. 357, 1-10.
14. + exchanger that are important for proton translocation, Mol. Cell. Biochem. 254, 117-124.
15. +-peptide transporter PepT1, Biochem. Biophys. Res. Commun. 272, 726-730.
16. +)-coupled glutamate transporter from rat brain, J. Biol. Chem. 269, 19573-19577.
17. Grewer, C., Watzke, N., Wiessner, M., and Rauen, T. (2000) Glutamate translocation of the neuronal glutamate transporter EAAC1 occurs within milliseconds, Proc. Natl. Acad. Sci. U.S.A. 97, 9706-9711.
18. Chen, C., and Okayama, H. (1987) High-efficiency transformation of mammalian cells by plasmid DNA, Mol. Cell. Biol. 7, 2745-2752.
19. Wadiche, J. I., Amara, S. G., and Kavanaugh, M. P. (1995) Ion fluxes associated with excitatory amino acid transport, Neuron 15, 721-728.
20. Watzke, N., Bamberg, E., and Grewer, C. (2001) Early intermediates in the transport cycle of the neuronal excitatory amino acid carrier EAAC1, J. Gen. Physiol. 117, 547-562.
21. Bergles, D. E., Tzingounis, A. V., and Jahr, C. E. (2002) Comparison of coupled and uncoupled currents during glutamate uptake by GLT-1 transporters, J. Neurosci. 22, 10153-10162.
22. Canepari, M., Nelson, L., Papageorgiou, G., Corrie, J. E. T., and Ogden, D. (2001) Photochemical and pharmacological evaluation of 7-nitroindolinyl- and 4-methoxy-7-nitroindolinyl-amino acids as novel, fast caged neurotransmitters, J. Neurosci. Methods 112, 29-42.
23. Ryan, R. M., and Vandenberg, R. J. (2002) Distinct conformational states mediate the transport and anion channel properties of the glutamate transporter EAAT-1, J. Biol. Chem. 277, 13494-13500.
24. Wadiche, J. I., and Kavanaugh, M. P. (1998) Macroscopic and microscopic properties of a cloned glutamate transporter/chloride channel, J. Neurosci. 18, 7650-7661.
25. Brocke, L., Bendahan, A., Grunewald, M., and Kanner, B. I. (2002) Proximity of two oppositely oriented reentrant loops in the glutamate transporter GLT-1 identified by paired cysteine mutagenesis, J. Biol. Chem. 277, 3985-3992.
26. Glusker, J. P. (1991) Structural aspects of metal liganding to functional groups in proteins, Adv. Protein Chem. 42, 1-76.
27. Fersht, A. (1999) Structure and mechanism in protein science, W. H. Freeman and Co., New York.
28. +)-coupled glutamate transporter from rat brain, J. Biol. Chem. 270, 17093-17097.
29. Bendahan, A., Armon, A., Madani, N., Kavanaugh, M. P., and Kanner, B. I. (2000) Arginine 447 plays a pivotal role in substrate interactions in a neuronal glutamate transporter, J. Biol. Chem. 275, 37436-37442.
30. Wadiche, J. I., Arriza, J. L., Amara, S. G., and Kavanaugh, M. P. (1995) Kinetics of a human glutamate transporter, Neuron 14, 1019-1027.
31. Yernool, D., Boudker, O., Jin, Y., and Gouaux, E. (2004) Structure of a glutamate transporter homologue from Pyrococcus horikoshii, Nature 431, 811-818.
32. Grunewald, M., Bendahan, A., and Kanner, B. I. (1998) Biotinylation of single cysteine mutants of the glutamate transporter GLT-1 from rat brain reveals its unusual topology, Neuron 21, 623-632.