The rice nucellin gene ortholog OsAsp1 encodes an active aspartic protease without a plant-specific insert and is strongly expressed in early embryo

Plant and Cell Physiology

Volumn 46, Issue 1, 2005, Pages 87-98

  Bi, Xuezhi ^a,b   Khush, Gurdev S ^a   Bennett, John ^a  

^a INTERNATIONAL RICE RESEARCH INSTITUTE   (Philippines)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Aspartic protease; Embryo; Gene expression; Recombinant protein; Rice (Oryza sativa L.)

Indexed keywords

ASPARTIC PROTEINASE; MESSENGER RNA; NUCELLIN; PLANT DNA; PLANT RNA; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BARLEY; CHROMOSOME MAP; COMPARATIVE STUDY; ENZYMOLOGY; GENE; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PEPTIDE MAPPING; PHYLOGENY; PLANT CHROMOSOME; PRENATAL DEVELOPMENT; RICE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ASPARTIC ENDOPEPTIDASES; BASE SEQUENCE; CHROMOSOME MAPPING; CHROMOSOMES, PLANT; CLONING, MOLECULAR; DNA, PLANT; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; HORDEUM; MOLECULAR SEQUENCE DATA; ORYZA SATIVA; PEPTIDE MAPPING; PHYLOGENY; PLANT PROTEINS; RECOMBINANT PROTEINS; RNA, MESSENGER; RNA, PLANT; SEQUENCE HOMOLOGY, AMINO ACID;

ARABIDOPSIS; BACTERIA (MICROORGANISMS); DICOTYLEDONEAE; ESCHERICHIA; HORDEUM VULGARE SUBSP. VULGARE; LILIOPSIDA; ORYZA SATIVA;

EID: 14644393639     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/pcp/pci002     Document Type: Article

References (44)

1. Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796-815.
2. Asakura, T., Matsumoto, I., Funaki, J., Arai, S. and Abe, K. (2000) The plant aspartic proteinase-specific polypeptide insert is not directly related to the activity of oryzasin. Eur. J. Biochem. 267: 5115-5122.
3. Asakura, T., Watanabe, H., Abe, K. and Arai, S. (1995) Rice aspartic proteinase, oryzasin, expressed during seed ripening and germination, has a gene organization distinct from those of animal and microbial aspartic proteinases. Eur. J. Biochem. 232: 77-83.
4. Beers, E.P., Woffenden, B.J. and Zhao, C.S. (2000) Plant proteolytic enzymes: possible roles during programmed cell death. Plant Mol. Biol. 44: 399-415.
5. Chen, F. and Foolad, M.R. (1997) Molecular organization of a gene in barley which encodes a protein similar to aspartic protease and its specific expression in nucellar cells during degeneration. Plant Mol. Biol. 35: 821-831.
6. Chen, F., and Foolad, M.R. (1999) Nucellar-cell-specific expression of a lipid transfer protein gene in barley (Hordeum vulgare L.). Plant Cell Rep. 18: 445-450.
7. Chen, L.Z., Miyazaki, C., Kojima, A., Saito, A. and Adachi, T. (1999) Isolation and characterization of a gene expressed during early embryo sac development in apomictic guinea grass (Panicum maximum). J. Plant Physiol. 154: 55-62.
8. DeBlock, M. and Debrouwer, D. (1993) RNA-RNA in situ hybridization using digoxigenin-labeled probes, the use of high molecular weight polyvinyl alcohol in the alkaline phosphatase indoxyl-nitroblue tetrazolium. Anal. Biochem. 216: 88-89
9. Dellaporta, S.L., Wood, J. and Hicks, J.B. (1983) A plant DNA minipreparation, version 11. Plant Mol. Biol. Rep. 1: 19-21.
10. Doan, D.N.P., Linnestad, C. and Olsen, O-A. (1996) Isolation of molecular markers from the barley endosperm coenocyte and the surrounding cell layers. Plant Mol. Biol. 31: 877-886.
11. Dominguez, F. and Cejudo, F.J. (1998) Germination-related genes encoding proteolytic enzymes are expressed in the nucellus of developing wheat grains. Plant J. 15: 569-574.
12. Dominguez, F., Moreno, J., and Cejudo, FJ. (2001) The nucellus degenerates by a process of programmed cell death during the early stages of wheat grain development. Planta 213: 352-360.
13. Dong, Y.H., Kvarnheden, A., Yao, J.L., Sutherland, P.W., Atkinson, R.G., Morris, B.A. and Gardner, R.C. (1998) Identification of pollination-induced genes from the ovary of apple (Malus domestica). Sexual Plant Reprod. 11: 277-283.
14. Gattiker, A., Gasteiger, E. and Bairoch A. (2002) ScanProsite, a reference implementation of a PROSITE scanning tool. Appl. Bioinformatics 1: 107-108.
15. Hayashi, M., Aoki, M., Kato, A., Kondo, M. and Nishimura, M. (1996) Transport of chimeric proteins that contain a carboxy-terminal targeting signal into plant microbodies. Plant J. 10: 225-234.
16. Hayashi, M., Aoki, M., Kondo, M. and Nishimura, M. (1997) Changes in targeting efficiencies of proteins to plant microbodies caused by amino acid substitutions in the carboxy-terminal tripeptide. Plant Cell Physiol. 38: 759-768.
17. He, X. and Kermode, A.R. (2003) Nuclease activities and DNA fragmentation during programmed cell death of megagametophyte cells of white spruce (Picea glauca) seeds. Plant Mol. Biol. 51: 509-521.
18. Jackson, D.P. (1991) In situ hybridization in plants. In Molecular Plant Pathology: A Practical Approach. Edited by Bowles, D.J. Gurr, S.J. and Mcphereson, M. pp. 163-174. Oxford University Press,
19. Jung, W., Skadsen, R.W. and Peterson, D.M. (1997) Cloning and characterization of an early embryogenesis gene, EEA1 (accession no. AF017430), from barley (PGR97-184). Plant Physiol. 115: 1730.
20. Kervinen, J., Tobin, G.J., Costa, J., Waugh, D.S., Wlodawer, A. and Zdanov, A. (1999) Crystal structure of plant aspartic proteinase prophytepsin: inactivation and vacuolar targeting. EMBO J. 18: 3947-3955
21. Kikuchi, S., Satoh, K., Nagata, T., Kawagashira, N., Doi, K., et al. (2003) Collection, mapping, and annotation of over 28, 000 cDNA clones from japonica rice. Science 301: 376-379.
22. Lindholm, P., Kuittinen, T., Sorri, O., Guo, D., Merits, A., Tormakangas, K. and Runeberg-Roos, P. (2000) Glycosylation of phytepsin and expression of dad1, dad2 and ost1 during onset of cell death in germinating barley scutella. Mech. Dev. 93: 169-173.
23. Linnestad, C., Doan, D.N.P., Brown, R.C., Lemmon, B.E., Meyer, D.J., Jung, R. and Olsen, O-A (1998) Nucellain, a barley homolog of the dicot vacuolar-processing protease, is localized in nucellar cell walls. Plant Physiol. 118: 1169-1180.
24. Matsuoka, K. and Neuhaus, J.-M. (1999) Cis-elements of protein transport to the plant vacuoles. J. Exp. Bot. 50: 165-174.
25. Mullen, R.T. (2002) Targeting and import of matrix proteins into peroxisomes. In Plant Peroxisomes, Biochemistry, Cell Biology and Biotechnological Applications. Edited by Baker, A. and Graham, I. pp 339-385. Kluwer Academic Publishers, Dordrecht.
26. Mutlu, A. and Gal, S. (1999) Plant aspartic proteinases: enzymes on the way to a function. Physiol. Plant. 105: 569-576.
27. Nakai, K. and Horton, P. (1999). PSORT, a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends Biochem. Sci. 24: 34-35.
28. Nakano, T., Murakami, S., Shoji, T., Yoshida, S., Yamada, Y. and Sato, F. (1997) A novel protein with DNA binding activity from tobacco chloroplast nucleoids. Plant Cell 9: 1673-1682.
29. Rawlings, N.D. and Barrett, A.J. (1995) Families of aspartic peptidases and those of unknown catalytic mechanism. Methods Enzymol. 24: 105-120.
30. Runeberg-Roos, P., Kervinen, J., Kovaleva, V., Raikhel, N.V. and Gal, S. (1994) The aspartic proteinase of barley is a vacular enzyme that processes probarley leetin in vitro. Plant Physiol. 105: 321-329.
31. Runeberg-Roos, P. and Saarma, M. (1998) Phytepsin, a barley vacuolar aspartic proteinase, is highly expressed during autolysis of developing tracheary elements and sieve cells. Plant J. 15: 139-145.
32. Runeberg-Roos, P., Törmäkangas, K. and Östman, A. (1991) Primary structure of a barley-grain aspartic proteinase, a plant aspartic proteinase resembling mammalian cathepsin D. Eur. J. Biochem. 202: 1021-1027.
33. Ruzin, S.E. (ed.) (1999) Plant Microtechnique and Microscopy, pp. 193-204. Oxford University Press, New York.
34. Schiefthaler, U., Bansubramanian, S., Sieber, P., Chevakier, D., Wissman, E. and Schneitz, K. (1999) Molecular analysis of NOZZLE, a gene involved in pattern formation and early sporogeneisi during sex organ development in Arabidopsis thaliana. Proc. Natl Acad. Sci. USA 96: 11664-11669.
35. Simões, I. and Faro, C. (2004) Structure and function of plant aspartic proteinases. Eur. J. Biochem. 271: 2067-2075.
36. Sturaro, M., Linnestad, C., Kleinhofs, A., Olsen, O.-A. and Doan, D.N.P. (1998) Characterization of a cDNA encoding a putative extensin from developing barley grains (Hordenm vulgare L.). J. Exp. Bot. 49: 1935-1944.
37. 2+-dependent cysteine protease is associated with anoxia-induced root tip death in maize. J. Exp. Bot. 51: 721-730.
38. Törmäkangas, K., Hadlington, J.L., Pimpl, P., Hillmer, S., Brandizzi, F., Teeri, T.H. and Denecke, J. (2001) A vacuolar sorting domain may also influence the way in which proteins leave the endoplasmic reticulum. Plant Cell 13: 2021-2032.
39. Törmäkangas, K., Kervinen, J., Östman, A. and Teeri, T. (1994) Tissue-specific localization of aspartic proteinase in developing and germinating barley grains. Planta 195: 116-125.
40. Wu, H.M. and Cheun, A.Y. (2000) Programmed cell death in plant reproduction. Plant Mol. Biol. 44: 267-281.
41. Xia, Y., Suzuki, H., Borevitz, J., Blount, J., Guo, Z., Patel, K., Dixon, R.A. and Lamb, C. (2004) An extracellular aspartic protease functions in Arabidopsis disease resistance signaling. EMBO J. 23: 980-988.
42. Xu, F.X. and Chye, M.L. (1999) Expression of cysteine proteinase during developmental events associated with programmed cell death in brinjal. Plant J. 17: 321-327.
43. Xu, J., Yang, D., Domingo, J., Ni, J. and Huang, N. (1998) Screening for overlapping bacterial artificial chromosome clones by PCR analysis with an arbitrary primer. Proc. Natl Acad. Sci. USA 95: 5661-5666.
44. Xu, P. and Roossinck, M.J. (2000) Cucumber mosaic virus D satellite RNA-induced programmed cell death in tomato. Plant Cell 12: 1079-1092.