Investigations of Phagosomes, Mitochondria, and Acidic Granules in Human Neutrophils Using Fluorescent Probes

Cytometry Part B - Clinical Cytometry

Volumn 51, Issue 1, 2003, Pages 21-29

  Bassøe, Carl Fredrik ^a,b,c   Li, Nianyu ^b   Ragheb, Kathy ^b   Lawler, Gretchen ^b   Sturgis, Jennie ^b   Robinson, J Paul ^b  

^a HAUKELAND UNIVERSITY HOSPITAL   (Norway)

^b PURDUE UNIVERSITY   (United States)

^c UNIVERSITY OF BERGEN   (Norway)

Author keywords

Fluorescent probes; Granules; Human neutrophils; Mitochondria; Oxidative metabolism; Phagocytosis; Phagosomes

Indexed keywords

DIHYDRORHODAMINE 123; ETHIDIUM; FLUORESCENT DYE; HYDROETHIDIUM; LYSOTRACKER RED; MITOTRACKER GREEN; MITOTRACKER RED; REACTIVE OXYGEN METABOLITE; RHODAMINE 123; UNCLASSIFIED DRUG; ZYMOSAN;

ADULT; ARTICLE; AUTOFLUORESCENCE; CELL CULTURE; CELL GRANULE; CELL ORGANELLE; CELL SUBPOPULATION; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; DRUG ACCUMULATION; FEMALE; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; INTRACELLULAR TRANSPORT; LEUKOCYTE; LYSOSOME; LYSOSOMOTROPIC PROBE; MALE; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; NEUTROPHIL; NORMAL HUMAN; OXIDATIVE RESPONSE GRANULE; PHAGOCYTOSIS; PHAGOSOME; PRIORITY JOURNAL; RESPIRATORY BURST; STAINING;

EID: 1442349824     PISSN: 15524949     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.b.10003     Document Type: Article

References (23)

1. Hurst JK, Barrette WC. Leukocytic oxygen activation and microbicidal oxidative toxins. Crit Rev Biochem Mol Biol 1989;24:271-328.
2. Meischl C, Roos D. The molecular basis of chronic granulomatous disease. Springer Semin Immunopathol 1998;19:417-434.
3. Robinson JP. Oxygen and nitrogen reactive metabolites and phagocytic cells. In: Robinson JP, Babcock GF, editors. Phagocyte function: a guide for research and clinical evaluation. New York: John Wiley & Sons; 1998. p 217-252.
4. Roos D, de Boer M, Kuribayashi F, Meischl C, Weening RS, Segal AW, Ahlin A, Nemet K, Hossle JP, Bernatowska-Matuszkiewicz E, Middleton-Price H. Mutations in the X-linked and autosomal recessive forms of chronic granulomatous disease. Blood 1996;87:1663-1681.
5. Verhoeven AJ. The NADPH oxidase: lessons from chronic granulomatous disease neutrophils. Ann NY Acad Sci 1997;832:85-92.
6. Rothe G, Oser A, Valet G. Dihydrorhodamine 123: a new flow cytometric indicator for respiratory burst activity in neutrophil granulocytes. Naturwissenschaften 1988;75:354-355.
7. Rothe G, Valet G. Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2′,7′-dichlorofluorescin. J Leukoc Biol 1990;47:440-448.
8. Lehmann AK, Halstensen A, Bassøe C-F. Flow cytometric quantitation of human opsonin-dependent phagocytosis and oxidative burst responses to meningococcal antigens. Cytometry 1998;33:406-413.
9. Bassøe C-F, Smith I, Sørnes S, Halstensen A, Lehmann AK. Concurrent measurement of antigen- and antibody dependent oxidative burst and phagocytosis in monocytes and neutrophils. Methods Enzymol 2000;21:203-220.
10. Johnson LV, Walsh ML, Chen LB. Localization of mitochondria in living cells with rhodamine 123. Proc Natl Acad Sci USA 1980;77:990-994.
11. Darzynkiewicz Z, Staiano-Coico L, Melamed MR. Increased mitochondrial uptake of rhodamine 123 during lymphocyte stimulation. Proc Natl Acad Sci USA 1981;78:2383-2387.
12. Darzynkiewicz Z, Traganos F, Staiano-Coico L, Kapuscinski J, Melamed MR. Interaction of rhodamine 123 with living cells studied by flow cytometry. Cancer Res 1982;42:799-806.
13. Jankowski A, Grinstein S. A noninvasive fluorometric procedure for measurement of membrane potential. Quantification of the NADPH oxidase-induced depolarization in activated neutrophils. J Biol Chem 1999;274:26098-26104.
14. Klebanoff SJ. Iodination of bacteria: a bactericidal mechanism. J Exp Med 1967;126:1063-1078.
15. Badwey JA, Karnovsky ML. Active oxygen species and the functions of phagocytic leukocytes. Annu Rev Biochem 1980;49:695-726.
16. Hirai K, Moriguchi K, Wang GY. Human neutrophils produce free radicals from the cell-zymosan interface during phagocytosis and from the whole plasma membrane when stimulated with calcium ionophore A23187. Exp Cell Res 1991;194:19-27.
17. Poot M, Zhang YZ, Kramer JA, Wells KS, Jones LJ, Hanzel DK, Lugade AG, Singer VL, Haugland RP. Analysis of mitochondrial morphology and function with novel fixable fluorescent stains. J Histochem Cytochem 1996;44:1363-1372.
18. Poot M, Pierce RH. Analysis of mitochondria by flow cytometry. In: Darzynkiewicz Z, Crissman HA, Robinson JP, editors. Methods in cell biology. Vol 64. San Diego: Academic Press; 2001. p 117-128.
19. Via LE, Fratti RA, McFalone M, Pagan-Ramos E, Deretic D, Deretic V. Effects of cytokines on mycobacterial phagosome maturation. J Cell Sci 1998;111:897-905.
20. Robinson JP, Darzynkiewicz Z, Dean PN, Dressler L, Rabinovitch PS, Stewart C, Tanke H, Wheeless LL, editors. Current protocols in cytometry. New York: John Wiley & Sons; 2000. p A.2A.1.
21. Gilmore K, Wilson M. The use of chloromethyl-X-rosamine (Mitotracker Red) to measure loss of mitochondrial membrane potential in apoptotic cells is incompatible with cell fixation. Cytometry 1999;36:355-358.
22. Poot M. Analysis of intracellular organelles by flow cytometry and microscopy. In: Robinson JP, Darzynkiewicz Z, Dean PN, Dressler L, Rabinovitch PS, Stewart C, Tanke H, Wheeless LL, editors. Current protocols in cytometry. Vol 1. New York: John Wiley & Sons; 2000. p 9.4.1-9.4.19.
23. Borregaard N, Cowland JB. Granules of the human neutrophilic polymorphonuclear leukocyte. Blood 1997;89:3503-3521.