Development of multiplex PCR tests for identifying Shiga toxin subclasses in Escherichia coli;Opracowanie testów multiplex PCR do identyfikacji odmian toksyny Shiga wytwarzanych przez Escherichia coli

Medycyna Weterynaryjna

Volumn 60, Issue 2, 2004, Pages 207-210

  Osek, Jacek ^a,b   Weiner, Marcin ^a  

^a Zakad Mikrobiologii Panstwowego I   (Poland)

^b NONE   (Poland)

Author keywords

Escherichia coli; Multiplex PCR; Shiga toxins

Indexed keywords

RIBOSOME RNA; SHIGA TOXIN;

AMPLICON; ANALYTIC METHOD; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CATTLE; CONTROLLED STUDY; CORRELATION ANALYSIS; ESCHERICHIA COLI; GENE AMPLIFICATION; GENETIC ANALYSIS; GENOTYPE; INTERMETHOD COMPARISON; MOLECULAR WEIGHT; NONHUMAN; POLYMERASE CHAIN REACTION; REACTION ANALYSIS; RNA ANALYSIS; SWINE; VALIDATION PROCESS;

BACTERIA (MICROORGANISMS); BOS TAURUS; ESCHERICHIA COLI; SUS SCROFA;

EID: 1442289934     PISSN: 00258628     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Bertschinger H. U., Bachmann M., Mettler C., Pospischil A., Schraner A., Stamm M., Sydler T., Wild P.: Adhesive fimbriae produced in vivo by Escherichia coli O139:K12(B):H1 associated with enterotoxaemia in pigs. Vet. Microbiol. 1990, 25, 267-281.
2. Beutin L., Montenegro M. A., Orskov I., Orskov F., Prada J., Zimmermann S., Stephan R.: Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli. J. Clin. Microbiol. 1989, 27, 2559-2564.
3. Cebula T. A., Payne W. I., Feng P.: Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J. Clin. Microbiol. 1995, 33, 248-250.
4. Ehresmann C., Stiegler P., Fellner P., Ebel P.: The determination of the primary structure of the 16S ribosomal RNA of Escherichia coli. 2. Nucleotide sequence of products from partial enzymatic hydrolysis. Biochemie 1972, 54, 901-967.
5. Eklund M., Leino K., Siitonen A.: Clinical Escherichia coli strains carrying stx genes: stx variants and stx-positive virulence profiles. J. Clin. Microbiol. 2002, 40, 4585-4593.
6. Fratamico P. M., Bagi L. K., Pepe T.: A multiplex polymerase chain reaction assay for rapid detection and identification of Escherichia coli O157:H7 in foods and bovine feces. J. Food Prot. 2000, 63, 1032-1037.
7. Gallien P., Much C., Perlberg K. W., Protz D.: Einsatz von Nylonmembranen zur spezifischen Isolierung von Escherichia coli O157 mittels DNA-Sonden und Prüfung auf STEC-Gruppenzugehörigkeit. Berl. Münch. Tierärtzl. Wschr. 1999, 112, 58-63.
8. Imberechts H., De Greve H., Lintermans P.: The pathogenesis of edema disease in swine. Vet. Microbiol. 1992, 31, 221-233.
9. Ito H., Terai A., Kurazono H., Takeda Y., Nishihuchi M.: Cloning and nucleotide sequencing of Vero toxin 2 variant genes from Escherichia coli O91:H21 isolated from a patient with the hemolytic uremic syndrome. Microb. Pathogen. 1990, 8, 867-876.
10. Jackson M. P., Neill R. J., O'Brien A. D., Holmes R. K., Newland J. W.: Nucleotide sequence analysis and comparision of the structural genes for Shiga-like toxin I and Shiga-like toxin II encoded by bacteriophages form Escherichia coli. FEMS Microbiol. Lett. 1987, 44, 109-114.
11. Marques L. R. M., Peiris J. S. M., Cryz S. J., O'Brien A. D.: Escherichia coli strains isolated from pigs produce a variant of Shiga-like toxin II. FEMS Microbiol. Lett. 1987, 44, 33-38.
12. Meng J., Zhao S., Doyle M. P., Mitchell S. E., Kresovich S.: A multiplex PCR for identifying Shiga-like toxin-producing Escherichia coli O157:H7. Lett. App. Microbiol. 1997, 24, 172-176.
13. Osek J., Dacko J.: Development of a PCR-based method for specific identification of genotypic markers of Shiga toxin-producing Escherichia coli strains. J. Vet. Med. B 2002, 48, 771-778.
14. Osek J.: Prevalence of virulence factors of Escherichia coli strains isolated from diarrheic and healthy piglets after weaning. Vet. Microbiol. 1999, 68, 209-217.
15. Osek J., Gallien P., Protz D.: Characterization of Shiga toxin-producing Escherichia coli strains isolated from calves in Poland. Comp. Immun. Microbiol. Infect. Dis. 2002, 23, 267-276.
16. O157. J. Clin. Microbiol. 1998, 36, 598-602.
17. Paton J. C., Paton A. W.: Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin. Microbiol. Rev. 1998, 11, 450-479.
18. Paton A. W., Paton J. C., Goldwater P. N., Heuzenroeder M. W., Manning P. A.: Sequence of a variant Shiga-like type I operon of Escherichia coli O111:H-. Gene 1993, 129, 87-92.
19. Schmidt H., Scheff J., Morabito S., Caprioli A., Wieler L. H., Karch H.: A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons. Appl. Env. Microbiol. 2000, 66, 1205-1208.
20. Tarr P. I.: Escherichia coli O157:H7: clinical, diagnostic, and epidemiological aspects of human infection. Clin. Infect. Dis. 1995, 20, 1-8.
21. Wang G., Clark C. G. and Rodgers F. G.: Detection of Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 sero-type, and components of the type 2 Shiga toxin family by multiplex PCR. J. Clin. Microbiol. 2002, 40, 3613-3619.
22. Zhang W., Bielaszewska M., Kuczius T., Karch H.: Identification, characterization, and distribution of a Shiga toxin 1 gene variant (stx1c) in Escherichia coli strains isolated from humans. J. Clin. Microbiol. 2002, 40, 1441-1446.