Segmental variation in the in vitro cell metabolism of nucleus pulposus cells isolated from a series of bovine caudal intervertebral discs

Spine

Volumn 30, Issue 5, 2005, Pages 505-511

  Wiseman, Mike A ^a,b   Birch, Helen L ^a   Akmal, Mohammed ^b   Goodship, Allen E ^a,b  

^a ROYAL VETERINARY COLLEGE   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Alginate culture; Cells; Intervertebral disc; Metabolism; Nucleus pulposus

Indexed keywords

ALGINIC ACID; CHONDROITIN SULFATE; COLLAGEN; COLLAGEN TYPE 1; DNA; GAG PROTEIN; HISTAMINE; SULFATE; THYMIDINE; WATER;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ARTICULAR CARTILAGE; BIOMECHANICS; CELL ISOLATION; CELL METABOLISM; CELL PROLIFERATION; CELL REGENERATION; EXTRACELLULAR MATRIX; IN VITRO STUDY; INTERVERTEBRAL DISK; MONOLAYER CULTURE; NONHUMAN; NUCLEUS PULPOSUS; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SPINE; TISSUE DISTRIBUTION; WATER CONTENT;

ANIMALS; CATTLE; CAUDA EQUINA; CELLS, CULTURED; INTERVERTEBRAL DISK;

EID: 14344250141     PISSN: 03622436     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.brs.0000154615.22311.66     Document Type: Article

References (47)

1. Buckwalter JA. Aging and degeneration of the human intervertebral disc. Spine 1995;20:1307-14.
2. Videman T, Battie MC. The influence of occupation on lumbar degeneration. Spine 1999;24:1164-8.
3. Ala-kokko L. Genetic risk factors for lumbar disc disease. Ann Med 2000;34:42-7.
4. Pope MH, Goh KL, Magnusson ML. Spine ergonomics. Annu Rev Biomed Eng 2002;4:49-68.
5. Sizer PS, Matthijs O, Phelps V. Influence of age on the development of pathology. Curr Rev Pain 2000;4:362-73.
6. Luoma K, Riihimaki H, Luukkonen R, et al. Low back pain in relation to lumbar disc degeneration. Spine 2000;25:487-92.
7. Salminen JJ, Erkintalo MO, Pentti J, et al. Recurrent low back pain and early disc degeneration in the young. Spine 1999;24:1316-21.
8. van Tulder MW, Assendelft WJ, Koes BW, et al. Spinal radiographic findings and non-specific low back pain: a systematic review of observational studies. Spine 1997;22:427-34.
9. Dong F, Dai K, Hou X. An experimental study on the relationship between disc nutrition and disc degeneration. Zhonghua Wai Ke Za Zhi 1995;33:147-50.
10. Horner HA, Urban JP. 2001 Volvo award winner in basic science studies: effect of nutrient supply on the viability of cells from the nucleus pulposus of the intervertebral disc. Spine 2001;26:2543-9.
11. Grob D, Humke T, Dvorak J. Direct pediculo-body fixation in the cases of spondylolisthesis with advanced intervertebral disc degeneration. Eur Spine J 1996;5:281-5.
12. Phillips FM, Reuben J, Wetzel FT. Intervertebral disc degeneration adjacent to a lumbar fusion: an experimental rabbit model. J Bone Joint Surg Br 2002;84:289-94.
13. Chow DH, Luk KD, Evans JH, et al. Effects of short anterior lumbar interbody fusion on biomechanics of neighbouring unfused segments. Spine 1996;21:549-55.
14. Maiman DJ, Kumaresan S, Yoganandan N, et al. Biomechanical effects of anterior cervical spine fusion on adjacent segments. Biomed Mater Eng 1999;9:27-38.
15. Frick SL, Hanley EN, Meyer RA, et al. Lumbar intervertebral disc transfer: a canine study. Spine 1994;19:1826-34.
16. Nomura T, Mochida J, Okuma M, et al. Nucleus pulposus allograft retards intervertebral disc degeneration. Clin Orthop 2001;389:94-101.
17. Okuma M, Mochida J, Nishimura K, et al. Reinsertion of stimulated nucleus pulposus cells retards intervertebral disc degeneration: an in vitro and in vivo experimental study. J Orthop Res 2000;18:988-97.
18. Nishimura K, Mochida J. Percutaneous re-insertion of the nucleus pulposus: an experimental study. Spine 1998;23:1531-9.
19. Lee JY, Hall R, Pelinkovic D, et al. New use of three dimensional pellet culture system for human intervertebral disc cells. Spine 2001;26:1316-22.
20. Maldonado BA, Oegema TRJ. Initial characterisation of the metabolism of intervertebral disc cells encapsulated in microspheres. J Orthop Res 1992;10:677-90.
21. Baer AE, Wang JY, Kraus VB, et al. Collagen gene expression and mechanical properties of intervertebral disc cell-alginate cultures. J Orthop Res 2001;19:2-10.
22. Skaggs DL, Weidenbaum M, Ratcliffe A, et al. Regional variation in tensile properties and biochemical composition of the human annulus fibrosus. Spine 1994;19:1310-9.
23. Ebara S, Iatridis JC, Setton LA, et al. Tensile properties of non-degenerate human annulus fibrosus. Spine 1996;21:452-61.
24. Quinnell RC, Stockdale HR, Willis DS. Observations of pressure within normal discs in the lumbar spine. Spine 1983;8:166-9.
25. Chelberg MK, Banks GM, Geiger DF, et al. Identification of heterogeneous cell populations in normal human intervertebral disc. J Anat 1995;186:43-53.
26. Chiba K, Andersson GB, Masuda K, et al. Metabolism of the extracellular matrix formed by intervertebral disc cells cultured in alginate. Spine 1997;22:2885-93.
27. Chiba K, Andersson GB, Masuda K, et al. A new culture system to study the metabolism of the intervertebral disc in vitro. Spine 1998;23:1821-8.
28. Wang JY, Baer AE, Kraus VB, et al. Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture. Spine 2001;26:1747-51.
29. Horner HA, Roberts S, Bielby RC, et al. Cells from different regions of the intervertebral disc: effect of culture system on matrix expression and cell phenotype. Spine 2002;27:1018-28.
30. th Orthopedic Research Society, San Francisco, CA, February 25-28, 2002:26.
31. Wiseman MA, Henson F, Lee DA, et al. Dynamic strain inhibits nitric oxide in equine chondrocytes isolated from different joint regions. Equine J 2003;35:451-6.
32. Little CB, Ghosh P. Variation in proteoglycan metabolism by articular chondrocytes in different joint regions is determined by post natal mechanical loading. Osteoarthritis Cartilage 1997;5:49-62.
33. Brinckmann P. Disruption of bovine tail discs resulting from compressive testing dependent on flexion, state of hydration, and stress range. Spine 2002;27:217-9.
34. Race A, Broom ND, Robertson P. Effect of loading rate and hydration on the mechanical properties of the disc. Spine 2000;25:662-9.
35. Ishihara H, McNally DS, Urban JP, et al. Effects of hydrostatic pressure on matrix synthesis in different regions of the intervertebral disk. J Appl Physiol 1996;80:839-46.
36. Razaq S, Urban JP, Wilkins RJ. Regulation of intracellular pH by bovine intervertebral disc cells. Cell Physiol Biochem 2001;10:109-15.
37. Alini M, Li W, Markovic P, et al. The potential and limitations of a cell-seeded collagen/hyaluronan scaffold to engineer an intervertebral disc-like matrix. Spine 2003;28:446-54.
38. Wiseman MA, Birch HL, Goodship AE. The mechanical sensitivity and metabolic activity of disc nuclear cells are dependent upon disc level. Society of Back Pain Research, November 11-12, 1999, Cardiff International Arena, UK.
39. Taylor TK, Melrose J, Burkhardt D, et al. Spinal biomechanics and aging are major determinants of the proteoglycan metabolism of intervertebral disc cells. Spine 2000;25:3014-20.
40. 35S-labelled proteoglycans complexed to alcian blue by rapid filtration in multiwell plates. Anal Biochem 1994;217:167-75.
41. Roa J, Otto WR. Fluorimetric DNA assay for cell growth estimation. Anal Biochem 1992;207:675-81.
42. Kim YJ, Sah RL, Doong JY, et al. Fluorometric assay of DNA in cartilage explants using Hoechst 33258. Anal Biochem 1988;174:168-76.
43. Farndale RW, Buttle DJ, Barrett AJ. Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylene blue. Biochim Biophys Acta 1986;883:173-7.
44. Bergman I, Loxley R. Two improved and simplified methods for the spectrophotometric determination of hydroxyproline. Anal Chem 1963;35:1961-5.
45. Bannister DW, Burns AB. Adaption of the Bergman and Loxley technique for hydroxyproline determination to the autoanalyser and its use in determining plasma hydroxyproline in the domestic fowl. Analyst 1970;95:596-600.
46. Light ND, Bailey AJ. The chemistry of the collagen cross links: purification and characterization of cross-linked polymeric peptide material from mature collagen containing unknown amino acids. Biochem J 1980;185:373-81.
47. Laemmli UK. Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-2.