Epitope mapping of the antigenic protein TsNTxP from Tityus serrulatus scorpion venom using mouse, rabbit and sheep antibodies

Toxicon

Volumn 44, Issue 6, 2004, Pages 617-624

  Mendes, T M ^a   Maria, W S ^b   Granier, C ^c   Chavez Olortegui C ^a   Kalapothakis, E ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b FUNDAÇÃO EZEQUIEL DIAS   (Brazil)

^c UNIV MONTPELLIER   (France)

Author keywords

Animal model; Anti venoms; Antigenic sites; Immunization; Non toxic protein; Recombinant; Synthetic peptides; Tityus serrulatus; TsNTxP

Indexed keywords

ANTIBODY; ANTIGEN; ANTIGEN TSNTXP; SCORPION VENOM; UNCLASSIFIED DRUG; PEPTIDE; TSNTXP PROTEIN, TITYUS SERRULATUS;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; EPITOPE MAPPING; FEMALE; IMMUNOREACTIVITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; RABBIT; SERODIAGNOSIS; SHEEP; ANIMAL; ANTIBODY COMBINING SITE; BAGG ALBINO MOUSE; COMPARATIVE STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; IMMUNOLOGY; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; SCORPION;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; BINDING SITES, ANTIBODY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; FEMALE; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; NEUTRALIZATION TESTS; PEPTIDES; RABBITS; SCORPION VENOMS; SCORPIONS; SHEEP;

EID: 14244252594     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2004.07.019     Document Type: Article

References (37)

1. L.M. Alvarenga, C.R. Diniz, C. Granier, and C. Chávez- Olórtegui Induction of neutralizing antibodies against Tityus serrulatus scorpion toxins by immunization with a mixture of defined synthetic epitopes Toxicon 40 2002 89 95
2. Amaral, C.F.S., Borges Dias, M., Campolina, D., 1994. Children with adrenergic manifestations of envenomation after Tityus serrulatus scorpion sting are protected from early anaphylactic antivenom reactions. Toxicon 32(2), 211-215.
3. + channels FEBS Lett. 486 2 2000 117 120
4. J.A. Campos, O.S. Silva, M. Lopez, and L. Freire-Maia Signs, symptoms and treatment of severe scorpion sting in children D. Eaker T. Wodstrom Natural Toxins 1980 Pergamon Press Oxford 61 68
5. J.L. Cardoso, W.H.R. Fan, A.A. Araujo, M. Santalucia, and J.M. Lopes Óbitos por escorpionismo no Brasil (1990-1993) Rev. Soc. Bras. Med. Trop. 28 1995 10
6. C. Chávez-Olótegui, A.M.M. Ferreira, M.do N. Cordeiro, W.S. Maria, M. Richardson, and C.R. Diniz Immunological and chemical properties of a non-toxic protein from the venom of the scorpion Tityus serrulatus (Lutz & Mello Campos, 1922) C. Bom M. Goyffon Envenoming and their Treatments 1996 Foundation Marcel Mérieux
7. C. Chávez-Olórtegui, E. Kalapothakis, A.M.B. Ferreira, A.P. Ferreira, and C.R. Diniz Neutralizing capacity of antibodies elicited by a non-toxic protein purified from the venom of the scorpion Tityus serrulatus Toxicon 35 1997 213 221
8. F. Couraud, E. Jover, J.M. Dubois, and H. Rochat Two types of scorpion toxin receptor sites, one related to the activation the other to the inactivation of the action potential sodium channel Toxicon 20 1982 9 16
9. P. Cupo, M.M. Azevedo-Marques, J.B. Menezes, and S.E. Hering Reações de hipersensibilidade imediatas após uso intravenoso de soros antivenenos: valor prognóstico dos testes de sensibilidade intradérmicos Rev. Inst. Med. Trop. Sao Paulo 33 2 1991 115 122
10. J.A. De Bin, J.E. Maggio, and G.R. Strichartz Purification and characterization of chlorotoxin, a chloride channels ligand from the venom of the scorpion Am. J. Physiol. 264 2 pt 1 1993 C361 C369
11. M.E. De Lima, and M.F. Martin-Euclaire The toxins purified from Tityus serrulatus (Lutz & Mello) venom J. Toxicol. Toxin Rev. 14 3 1995 457 481
12. R. Dhawan, A. Varshney, M.K. Mathew, and A.K. Lala BTK-2, a new inhibitor of the Kv1.1 potassium channel purified from Indian scorpion Buthus tamulus FEBS Lett. 539 1-3 2003 7 13
13. R. Frank Spot synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support Tetrahedron 48 1992 9217 9232
14. C. Goudet, I. Huys, E. Clynen, L. Schoofs, D.C. Wany, E. Waelkens, and J. Tytgat Electrophysiological characterization of Bmk M1, an alpha-like toxin from Buthus martensi karsch venom FEBS Lett. 495 1/2 2001 61 65
15. S.C.F. Guatimosim, V.F. Prado, C.R. Diniz, C. Chávez- Olórtegui, and E. Kalapothakis Molecular cloning and genomic analysis of TsNTxP an immunogenic protein from Tityus serrulatus scorpion venom Toxicon 37 1999 507 517
16. S.C.F. Guatimosim, E. Kalapothakis, C.R. Diniz, and C. Chávez-Olórtegui Induction of neutralizing antibodies against Tityus serrulatus toxins by immunization with a recombinant nontoxic protein Toxicon 38 1 2000 113 121
17. 50 J.H. Burn Biological Standardization 1937 Oxford University Press London 37 40
18. G.E. Kirsch, A. Skattebol, L.D. Possani, and A.M. Brown Modification of Na channel gating by an alpha scorpion toxin from Tityus serrulatus J. Gen. Physiol. 93 1 1989 67 83
19. Lacy, M.J., Voss, E.W. Jr., 1986. A modified method to induce immune polyclonal ascites fluid in BALB/c mice using Sp2/)-Ag14 cells. J Immunol Methods. 87(2), 169-177.
20. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacterophage T4 Nature 227 259 1970 680 685
21. 2+ channels in spermatogenic cells inhibit the sperm acrosome reaction Biochem. Biophys. Res. Commun. 300 2 2003 408 414
22. F. Molina, D. Laune, C. Gougat, B. Pau, and C. Granier Improved performances of Spot multiple peptide synthesis Peptide Res. 9 1996 151 155
23. + channels Biochem. Biophys. Res. Commun. 294 4 2002 562 568
24. Pardo-Lopez, L., Garcia-Valdes, J., Gurrola, G.B., Robertson, G.A., Possan, I L.D., 2002. Mapping the receptor site for ergtoxin, a specific blocker of ERG channels. FEBS Lett. 510(1-2), 45-49.
25. D.L. Possani Structure of scorpion toxins A.T. Tu Handbook of Natural Toxins 1984
26. D.L. Possani, A.C. Alargón, P.L. Fletcher Jr, and B.W. Erckson Purification and properties of mammalian toxins from the venom of Brazilian scorpion Tityus serrulatus (Lutz and Mello) Arch. Biochem. Biophys. 180 2 1977 394 403
27. D.L. Possani, B.M. Martin, M.D. Fletcher, and P.L. Fletcher Jr Discharge effect on pancreatic exocrine secretion produced by toxins purified from Tityus serrulatus scorpion venom J. Biol. Chem. 266 1991 3178 3185
28. + channels Eur. J. Biochem. 204 2 1999 287 300
29. N. Rezende, M. Bordes Dias, D. Campolina, C. Chávez- Olórtegui, C.R. Diniz, and C. Amaral Efficacy of antivenom therapy for neutralizing circulating venom antigens in patients stung by Tityus serrulatus scorpions Am. J. Trop. Med. Hyg. 52 1995 277 280
30. N.A. Rezende, C.F.S. Amaral, and L. Freire-Maia Immunotherapy for scorpion envenoming in Brazil Toxicon 36 11 1998 1507 1513
31. H. Rochat, C. Lecomte, J.M. Sabatier, and J. Van Rietschoten Synthetic peptides as tools to investigate the structure and pharmacology of potassium channel-acting short-chain scorpion toxins Biochimie 80 2 1998 151 154
32. S.V. Sampaio, C.J. Laure, and J.R. Giglio Isolation and characterization of the toxin proteins from the venom of the Brazilian scorpion Tityus serrulatus Toxicon 21 2 1983 265 277
33. 2+ release channel of sarcoplasmic reticulum by a novel scorpion toxin J. Biol. Chem. 266 29 1991 19135 19138
34. G.K. Wang, and G. Stricharz Simultaneous modifications of sodium channel gating by two scorpion toxins Biophys. J. 40 2 1982 175 179
35. X.C. Zeng, W.X. Li, S.Y. Zhu, F. Peng, Z.H. Zhu, K.L. Wu, and F.H. Yiang Cloning and characterization of a cDNA sequence encoding the precursor of a clhorotoxin-like peptide from Chinese scorpion Buthus martensi karsch Toxicon 38 8 2000 1009 1014
36. I. Zenouaki, R. Kharrat, J.-M. Sabatier, C. Devaux, H. Karoui, J. Van Rietschoten, M. El Ayeb, and H. Rochat In vivo protection against Androctonus australis hector scorpion toxin and venom by immunization with a synthetic analog of toxin II Vaccine 15 2 1997 187 194
37. E. Zlotkin Chemistry and pharmacology of buthinae scorpion venoms S. Bettini Arthropod Venoms 1978 317 369