An unusual mutation results in the replacement of diaminopimelate with lanthionine in the peptidoglycan of a mutant strain of Mycobacterium smegmatis

Journal of Bacteriology

Volumn 187, Issue 5, 2005, Pages 1612-1620

  Consaul, Sandra A ^a   Wright, Lori F ^a   Mahapatra, Sebabrata ^b   Crick, Dean C ^b   Pavelka Jr , Martin S ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^b Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; AMINO ACID; BETA LACTAM ANTIBIOTIC; CYSTATHIONINE BETA SYNTHASE; DETERGENT; DIAMINOPIMELIC ACID; GLYCINE; LANTHIONINE; PEPTIDE; PEPTIDOGLYCAN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO ACID SYNTHESIS; ANTIBIOTIC SENSITIVITY; ARTICLE; AUXOTROPHIC MUTANT; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM MUTANT; BINDING SITE; CARBOHYDRATE SYNTHESIS; CBS GENE; CONTROLLED STUDY; CROSS LINKING; METABOLITE; MUTATION; MYCOBACTERIUM LEPRAE; MYCOBACTERIUM SMEGMATIS; NONHUMAN; PRIORITY JOURNAL; RIBOSOME; TEMPERATURE;

ALANINE; ALLELES; AMINO ACID SEQUENCE; BASE SEQUENCE; CYSTATHIONINE BETA-SYNTHASE; DIAMINOPIMELIC ACID; MOLECULAR SEQUENCE DATA; MUTATION; MYCOBACTERIUM SMEGMATIS; PEPTIDOGLYCAN; SULFIDES;

EID: 14244250193     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.187.5.1612-1620.2005     Document Type: Article

References (31)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1987. Current protocols in molecular biology. Greene Publishing Associates, Media, Pa.
2. Azuma, I., D. W. Thomas, A. Adam, J. M. Ghuysen, R. Bonaly, J. F. Petit, and E. Lederer. 1970. Occurrence of N-glycolylmuramic acid in bacterial cell walls. A preliminary survey. Biochim. Biophys. Acta 208:444-451.
3. Bardarov, S., and W. R. Jacobs, Jr. 1995. Unpublished data.
4. Boyer, H., and D. Roulland-Dussoin. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41:459-472.
5. Braunstein, M., S. S. Bardarov, and W. R. Jacobs, Jr. 2002. Genetic methods for deciphering virulence determinants of Mycobacterium tuberculosis. Methods Enzymol. 358:67-99.
6. Brennan, P. J. 2003. Structure, function, and biogenesis of the cell wall of Mycobacterium tuberculosis. Tuberculosis (Edinburgh) 83:91-97.
7. Brennan, P. J., and H. Nikaido. 1995. The envelope of mycobacteria. Annu. Rev. Biochem. 64:29-63.
8. Cole, S. T., R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S. V. Gordon, K. Eiglmeier, S. Gas, C. E. Barry III, F. Tekaia, K. Badcock, D. Basham, D. Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, T. Feltwell, S. Gentles, N. Hamlin, S. Holroyd, T. Hornsby, K. Jagels, B. G. Barrell, et al. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537-544.
9. Consaul, S. A., W. R. Jacobs, Jr., and M. S. Pavelka, Jr. 2003. Extragenic suppression of the requirement for diaminopimelate in diaminopimelate auxotrophs of Mycobacterium smegmatis. FEMS Microbiol. Lett. 225:131-135.
10. Consaul, S. A., and M. S. Pavelka, Jr. 2004. Use of a novel allele of the Escherichia coli aacC4 aminoglycoside resistance gene as a genetic marker in mycobacteria. FEMS Microbiol. Lett. 234:297-301.
11. Doran, T., M. Tizard, D. Millar, J. Ford, N. Sumar, M. Loughlin, and J. Hermon-Taylor. 1997. IS900 targets translation initiation signals in Mycobacterium avium subsp. paratuberculosis to facilitate expression of its hed gene. Microbiology 143:547-552.
12. Fredriksen, Å., E. N. Vasstrand, and H. B. Jensen. 1991. Peptidoglycan precursor from Fusobacterium nucleatum contains lanthionine. J. Bacteriol. 173:900-902.
13. Ghuysen, J.-M. 1968. Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism. Bacteriol. Rev. 32:425-464.
14. Goffin, C., and J.-M. Ghuysen. 1998. Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs. Microbiol. Mol. Biol. Rev. 62:1079-1093.
15. Guder, A., I. Wiedemann, and H. G. Sahl. 2000. Posttranslationally modified bacteriocins-the lantibiotics. Biopolymers 55:62-73.
16. Janosik, M., V. Kery, M. Gaustadnes, K. N. Maclean, and J. P. Kraus. 2001. Regulation of human cystathionine beta-synthase by S-adenosyl-L- methionine: evidence for two catalytically active conformations involving an autoinhibitory domain in the C-terminal region. Biochemistry 40:10625-10633.
17. Jhee, K.-H., P. McPhie, and E. W. Miles. 2000. Domain architecture of the heme-independent yeast cystathionine β-synthase provides insight into mechanisms of catalysis and regulation. Biochemistry 39:10548-10556.
18. Lederer, E. 1971. The mycobacterial cell wall. Pure Appl. Chem. 25:135-165.
19. Mengin-Lecreulx, D., D. Blanot, and J. van Heijenoort. 1994. Replacement of diaminopimelic acid by cystathionine or lanthionine in the peptidoglycan of Escherichia coli. J. Bacteriol. 176:4321-4327.
20. Mengin-Lecreulx, D., T. Falla, D. Blanot, J. van Heijenoort, D. J. Adams, and I. Chopra. 1999. Expression of the Staphylococcus aureus UDP-N-acetyl-muramoyl-L-alanyl-D-glutamate:L-lysine ligase in Escherichia coli and effects on peptidoglycan biosynthesis and cell growth. J. Bacteriol. 181:5909-5914.
21. Nagasawa, T., H. Kanzaki, and H. Yamada. 1984. Cystathionine gamma-lyase of Streptomyces phaeochromogenes. The occurrence of cystathionine gamma-lyase in filamentous bacteria and its purification and characterization. J. Biol. Chem. 259:10393-10403.
22. Pavelka, M. S., Jr., and W. R. Jacobs, Jr. 1996. Biosynthesis of diaminopimelate, the precursor of lysine and a component of peptidoglycan, is an essential function of Mycobacterium smegmatis. J. Bacteriol. 178:6496-6507.
23. Pavelka, M. S., Jr., and W. R. Jacobs, Jr. 1999. Comparison of the construction of unmarked deletion mutations in Mycobacterium smegmatis, Mycobacterium bovis bacillus Calmette-Guérin, and Mycobacterium tuberculosis H37Rv by allelic exchange. J. Bacteriol 181:4780-4789.
24. Richaud, C., D. Mengin-Lecreulx, S. Pochet, E. J. Johnson, G. N. Cohen, and P. Marliere. 1993. Directed evolution of biosynthetic pathways. Recruitment of cysteine thioethers for constructing the cell wall of Escherichia coli. J. Biol. Chem. 268:26827-26835.
25. Schleifer, K. H., and O. Kandler. 1972. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36:407-477.
26. Snapper, S. B., R. E. Melton, S. Mustafa, T. Kleser, and W. R. Jacobs, Jr. 1990. Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol. Microbiol. 4:1911-1919.
27. Stover, C. K., V. F. de la Cruz, T. R. Fuerst, J. E. Burlein, L. A. Benson, L. T. Bennett, G. P. Bansal, J. F. Young, M. H. Lee, G. F. Hatfull, S. B. Snapper, R. G. Barletta, W. R. Jacobs, and B. R. Bloom. 1991. New use of BCG for recombinant vaccines. Nature 351:456-460.
28. Timm, J., M. Gomez, and I. Smith. 1999. Gene expression and regulation, p. 75. In C. Ratledge and J. Dale (ed.), Mycobacteria, molecular biology and virulence. Blackwell Publishing, Oxford, United Kingdom.
29. Umbarger, H. E. 1978. Amino acid biosynthesis and its regulation. Annu. Rev. Biochem. 47:533-606.
30. Wietzerbin, J., B. C. Das, J.-F. Petit, E. Lederer, M. Leyh-Bouille, and J.-M. Ghuysen. 1974. Occurrence of D-alanyl-(D)-meso-diaminopimelic acid and meso-diaminopimelyl-meso-diaminopimelic acid interpeptide linkages in the peptidoglycan of mycobacteria. Biochemistry 13:3471-3476.
31. Yagi, T., S. Mahapatra, K. Mikusova, D. C. Crick, and P. J. Brennan. 2003. Polymerization of mycobacterial arabinogalactan and ligation to peptidoglycan. J. Biol. Chem. 278:26497-26504.