Determination of 17α-hydroxyprogesterone in serum by liquid chromatography-tandem mass spectrometry and immunoassay

Scandinavian Journal of Clinical and Laboratory Investigation

Volumn 65, Issue 1, 2005, Pages 3-12

  Turpeinen, Ursula ^a   Itkonen, O ^a   Ahola, L ^b   Stenman, U H ^c  

^a HELSINKI UNIVERSITY CENTRAL HOSPITAL   (Finland)

^b Medix Laboratoriot Oy   (Finland)

^c UNIVERSITY OF HELSINKI   (Finland)

Author keywords

17 hydroxyprogesterone; 21 hydroxylase deficiency; Congenital adrenal hyperplasia; LC MS

Indexed keywords

ACETIC ACID ETHYL ESTER; ETHER; HYDROXYPROGESTERONE; METHYLPREDNISOLONE; STEROID 21 MONOOXYGENASE;

ARTICLE; CALIBRATION; CONGENITAL ADRENAL HYPERPLASIA; CONTROLLED STUDY; HUMAN; LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; PROGESTERONE BLOOD LEVEL; RADIOIMMUNOASSAY; REPRODUCIBILITY; SERUM; STEROID 21 MONOOXYGENASE DEFICIENCY; TANDEM MASS SPECTROMETRY; BLOOD; CHEMISTRY; IMMUNOASSAY; MASS SPECTROMETRY; METHODOLOGY; SENSITIVITY AND SPECIFICITY;

17-ALPHA-HYDROXYPROGESTERONE; CHROMATOGRAPHY, LIQUID; HUMANS; IMMUNOASSAY; MASS SPECTROMETRY; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 14044270128     PISSN: 00365513     EISSN: None     Source Type: Journal    
DOI: 10.1080/00365510410008421     Document Type: Article

References (16)

1. Pang S, Wallace MA, Hofman L, Thuline HC, Dorche C, Lyon ITC, et al. Worldwide experience in newborn screening for classical congenital adrenal hyperplasia due to 21-hydroxylase deficiency. Pediatrics 1988;81:866-74.
2. Boudi A, Giton F, Galons H, Eurly B, Villette JM, Soliman H, et al. Development of a plasma 17α-hydroxyprogesterone time resolved-fluorescence immunoassay involving a new biotinylated tracer. Steroids 2000;65:103-8.
3. Castracane VD, Gimpel T. Comparison of three methods for 17α-hydroxyprogesterone. J Clin Lab Anal 1997;11:179-85.
4. Nahoul K. Plasma 17-hydroxyprogesterone determination with two commercial immunoassays. J Steroid Biochem Molec Biol 1994;50:197-203.
5. Katayama M, Nakane R, Matsuda Y, Kaneko S, Kara I, Sato H. Determination of progesterone and 17-hydroxyprogesterone by high performance liquid chromatography after pre-column derivatization with 4,4-difluoro-5,7-dimethyl-4- bora-3a,4a-diaza-s-indacene-3-propionohydrazide. Analyst 1998;123:2339-42.
6. 4-steroids measured by HPLC-UV spectrometry in neonatal patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency. Horm Res 1990;33:27-34.
7. Apter D, Jänne O, Karvonen P, Vihko R. Simultaneous determination of five sex hormones in human serum by radioimmunoassay after chromatography on Lipidex-5000. Clin Chem 1976;22:32-8.
8. Jänne O, Apter D, Vihko R. Assay of testosterone, progesterone and 17α-hydroxyprogesterone in human plasma by radioimmunoassay after separation on hydroxyalkoxypropyl sephadex. J Steroid Biochem 1974;5:155-62.
9. Wong T, Shackleton CHL, Covey TR, Ellis G. Identification of the steroids in neonatal plasma that interfere with 17α-hydroxyprogesterone radioimmunoassays. Clin Chem 1992;38:1830-7.
10. Wudy SA, Hartmann M, Svoboda M. Determination of 17-hydroxyprogesterone in plasma by stable isotope dilution/benchtop liquid chromatography-tandem mass spectrometry. Horm Res 2000;53:68-71.
11. Kao PC, Machacek DA, Magera MJ, Lacey JM, Rinaldo P. Diagnosis of adrenal cortical dysfunction by liquid chromatography-tandem mass spectrometry. Ann Clin Lab Sci 2001;31:199-204.
12. Lai C-C, Tsai C-H, Tsai F-J, Wu J-Y, Lin W-D, Lee C-C. Monitoring of congenital adrenal hyperplasia by microbore HPLC-electrospray ionization tandem mass spectrometry of dried blood spots. Clin Chem 2002;48:354-6.
13. Annesley TM. Ion suppression in mass spectrometry. Clin Chem 2003;49:1041-4.
14. Passing H, Bablok W. A new biometrical procedure for testing the equality of measurements from two different analytical methods. J Clin Chem Clin Biochem 1983;21:709-20.
15. Lacey JM, Minutti CZ, Magera MJ, Tauscher AL, Casetta B, McCann M, et al. Improved specificity of newborn screening for congenital adrenal hyperplasia by second-tier steroid profiling using tandem mass spectrometry. Clin Chem 2004;50:621-5.
16. Joos PE, Van Ryckeghem M. Liquid chromatography-tandem mass spectrometry of some anabolic steroids. Anal Chem 1999;71:4701-10.