Identification of Fmoc-β-Ala-OH and Fmoc-β-Ala-amino acid-OH as new impurities in Fmoc-protected amino acid derivatives

Journal of Peptide Research

Volumn 65, Issue 1, 2005, Pages 90-97

  Hlebowicz, E ^a   Andersen, A J ^a   Andersson, L ^a   Moss, B A ^a  

^a PolyPeptide Laboratories (Sweden) AB   (Sweden)

Author keywords

Ala insertion; Fmoc Ala amino acid OH impurity; Fmoc Ala OH impurity; Peptide drug substance; Peptide synthesis

Indexed keywords

AMINO ACID DERIVATIVE; BETA ALANINE;

ARTICLE; CHEMICAL STRUCTURE; CONTAMINATION; DRUG IMPURITY; PRIORITY JOURNAL; PROTEIN ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ALANINE; AMINO ACIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DRUG CONTAMINATION; FLUORENES; HYDROXIDES; TIME FACTORS;

EID: 14044253473     PISSN: 1397002X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1399-3011.2004.00201.x     Document Type: Article

References (3)

1. Hlebowicz, E., Eriksson, J., Nilsson, M., Rebert, L., Svensson, T., Wittenmark, M., Andersen, A.J. & Andersson, L. (2004) Fmoc-β-Ala-OH and Fmoc-β-Ala-Amino acid-OH, two Newly Discovered Impurities in Fmoc-protected Amino Acid Derivatives. Poster presentation at 3rd International and 28th European Peptide Symposium, Prague, Czech Republic.
2. Dener, J.M., Fantauzzi, P.P., Kshirsagar, T.A., Kelly, D.E. & Wolfe, A.B. (2001) Large-scale syntheses of Fmoc-protected non-proteogenic amino acids: useful building blocks for combinatorial libraries. Org. Process Res. Dev. 5, 445-449.
3. Gross, H. & Bilk, L. (1968) Zur reaction von N-hydroxysuccinimid mit dicyclohexylcarbodiimid. Tetrahedron 5, 6935-6939.