Template DNA ratio can affect detection by genus-specific PCR-denaturing gradient gel electrophoresis of bacteria present at low abundance in mixed populations

Helicobacter

Volumn 10, Issue 1, 2005, Pages 80-82

  Zhang, Li ^a   Danon, Stephen ^b   Grehan, Martin ^a   Lee, Adrian ^a   Mitchell, Hazel ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

^b CSIRO   (Australia)

Author keywords

16S rRNA; DGGE; Helicobacter species

Indexed keywords

DNA; RNA 16S;

ADOLESCENT; ANIMAL TISSUE; ARTICLE; BACTERIAL COLONIZATION; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA TEMPLATE; DNA TEMPLATE RATIO; GENUS SPECIFIC POLYMERASE CHAIN REACTION DENATURING GEL ELECTROPHORESIS; INTERMETHOD COMPARISON; MIXED INFECTION; MIXED POPULATION; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; POPULATION ABUNDANCE; PRIORITY JOURNAL; RNA 16S GENE; RNA GENE; SPECIES IDENTIFICATION; SPECIES SPECIFIC POLYMERASE CHAIN REACTION DENATURING GEL ELECTORPHORESIS;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; CECUM; DNA, BACTERIAL; DNA, RIBOSOMAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FECES; GENES, RRNA; HELICOBACTER; HELICOBACTER INFECTIONS; MICE; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; TEMPLATES, GENETIC;

ANIMALIA; BACTERIA (MICROORGANISMS); HELICOBACTER; MURINAE;

EID: 13944256956     PISSN: 10834389     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1523-5378.2005.00294.x     Document Type: Article

References (7)

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