Cochlear gap junctions coassembled from Cx26 and 30 show faster intercellular Ca2+ signaling than homomeric counterparts

American Journal of Physiology - Cell Physiology

Volumn 288, Issue 3 57-3, 2005, Pages

  Sun, Jianjun ^a   Ahmad, Shoab ^a,b   Chen, Shanping ^a   Tang, Wenxue ^b   Zhang, Yanping ^b   Chen, Ping ^b   Lin, Xi ^a,b  

^a HOUSE EAR INSTITUTE   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Coassembly; Cochlea; Deafness

Indexed keywords

CALCIUM; CONNEXIN 26; CONNEXIN 30; GREEN FLUORESCENT PROTEIN;

ANIMAL TISSUE; ARTICLE; CALCIUM CELL LEVEL; CELL MEMBRANE PERMEABILITY; CELL STRAIN HEK293; COCHLEA; CONTROLLED STUDY; FEMALE; GAP JUNCTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMAGING SYSTEM; IMMUNODIFFUSION; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SIGNAL TRANSDUCTION;

ANIMALS; CALCIUM SIGNALING; CELL LINE; COCHLEA; CONNEXINS; FLUORESCENT DYES; GAP JUNCTIONS; HUMANS; MICE; MICE, KNOCKOUT; PROTEIN STRUCTURE, QUATERNARY; PROTEIN SUBUNITS; RECOMBINANT FUSION PROTEINS; TIME FACTORS;

EID: 13644252915     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.00341.2004     Document Type: Article

References (40)

1. Ahmad S, Chen S, Sun J, and Lin X. Connexins 26 and 30 are co-assembled to form gap junctions in the cochlea of mice. Biochem Biophys Res Commun 307: 362-368, 2003.
2. Bergoffen J, Scherer SS, Wang S, Scott MO, Bone LJ, Paul DL, Chen K, Lensch MW, Chance PF, and Fischbeck KH. Connexin mutations in X-linked Charcot-Marie-Tooth disease. Science 262: 2039-2042, 1993.
3. Bruzzone R, White TW, and Paul DL. Connections with connexins: the molecular basis of direct intercellular signaling. Eur J Biochem 238: 1-27, 1996.
4. Charles AC, Merrill JE, Dirksen ER, and Sanderson MJ. Intercellular signaling in glial cells: calcium waves and oscillations in response to mechanical stimulation and glutamate. Neuron 6: 983-992, 1991.
5. Cohen-Salmon M, Maxeiner S, Kruger O, Theis M, Willecke K, and Petit C. Expression of the connexin43- and connexin45-encoding genes in the developing and mature mouse inner ear. Cell Tissue Res 316: 15-22, 2004.
6. Diez JA, Ahmad S, and Evans WH. Assembly of heteromeric connexons in guinea-pig liver en route to the Golgi apparatus, plasma membrane and gap junctions. Eur J Biochem 262: 142-148, 1999.
7. Estivill X, Fortina P, Surrey S, Rabionet R, Melchionda S, D'Agruma L, Mansfield E, Rappaport E, Govea N, Mila M, Zelante L, and Gasparini P. Connexin-26 mutations in sporadic and inherited sensorineural deafness. Lancet 351: 394-398, 1998.
8. Evans WH and Martin PE. Gap junctions: structure and function. Mol Membr Biol 19: 121-136, 2002.
9. Forge A, Becker D, Casalotti S, Edwards J, Marziano N, and Nevill G. Gap junctions in the inner ear: comparison of distribution patterns in different vertebrates and assessement of connexin composition in mammals. J Comp Neurol 467: 207-231, 2003.
10. Grifa A, Wagner CA, D'Ambrosio L, Melchionda S, Bernardi F, Lopez-Bigas N, Rabionet R, Arbones M, Monica MD, Estivill X, Zelante L, Lang F, and Gasparini P. Mutations in GJB6 cause nonsyndromic autosomal dominant deafness at DFNA3 locus. Nat Genet 23: 16-18, 1999.
11. 2+ indicators with greatly improved fluorescence properties. J Biol Chem 260: 3440-3450, 1985.
12. Haas K, Sin WC, Javaherian A, Li Z, and Cline HT. Single-cell electroporation for gene transfer in vivo. Neuron 29: 583-591, 2001.
13. He DS, Jiang JX, Taffet SM, and Burt JM. Formation of heteromeric gap junction channels by connexins 40 and 43 in vascular smooth muscle cells. Proc Natl Acad Sci USA 96: 6495-6500, 1999.
14. Helms AW, Abney AL, Ben-Arie N, Zoghbi HY, and Johnson JE. Autoregulation and multiple enhancers control Math1 expression in the developing nervous system. Development 127: 1185-1196, 2000.
15. Iurato S, Franke K, Luciano L, Wermbter G, Pannese E, and Reale E. Intercellular junctions in the organ of Corti as revealed by freeze fracturing. Acta Otolaryngol (Stockh) 82: 57-69, 1976.
16. Jahnke K. The fine structure of freeze-fractured intercellular junctions in the guinea pig inner ear. Acta Otolaryngol Suppl (Stockh) 336: 1-40, 1975.
17. Kelsell DP, Dunlop J, Stevens HP, Lench NJ, Liang JN, Parry G, Mueller RF, and Leigh IM. Connexin 26 mutations in hereditary non-syndromic sensorineural deafness. Nature 387: 80-83, 1997.
18. Kikuchi T, Kimura RS, Paul DL, and Adams JC. Gap junctions in the rat cochlea: immunohistochemical and ultrastructural analysis. Anat Embryol (Berl) 191: 101-118, 1995.
19. Lautermann J, ten Cate WJ, Altenhoff P, Grummer R, Traub O, Frank H, Jahnke K, and Winterhager E. Expression of the gap-junction connexins 26 and 30 in the rat cochlea. Cell Tissue Res 294: 415-420, 1998.
20. Liu XZ, Xia XJ, Adams J, Chen ZY, Welch KO, Tekin M, Ouyang XM, Kristiansen A, Pandya A, Balkany T, Arnos KS, and Nance WE. Mutations in GJA1 (connexin 43) are associated with non-syndromic autosomai recessive deafness. Hum Mol Genet 10: 2945-2951, 2001.
21. Liu XZ, Xia XJ, Ke XM, Ouyang XM, Du LL, Liu YH, Angeli S, Telischi FF, Nance WE, Balkany T, and Xu LR. The prevalence of connexin 26 (GJB2) mutations in the Chinese population. Hum Genet 111: 394-397, 2002.
22. Liu XZ, Xia XJ, Xu LR, Pandya A, Liang CY, Blanton SH, Brown SD, Steel KP, and Nance WE. Mutations in connexin31 underlie recessive as well as dominant non-syndromic hearing loss. Hum Mol Genet 9: 63-67, 2000.
23. Locke D, Perusinghe N, Newman T, Jayatilake H, Evans WH, and Monaghan P. Developmental expression and assembly of connexins into homomeric and heteromeric gap junction hemichannels in the mouse mammary gland. J Cell Physiol 183: 228-237, 2000.
24. Manthey D, Banach K, Desplantez T, Lee CG, Kozak CA, Traub O, Weingart R, and Willecke K. Intracellular domains of mouse connexin26 and -30 affect diffusional and electrical properties of gap junction channels. J Membr Biol 181: 137-148, 2001.
25. Martinez-Wittinghan FJ, Sellitto C, Li L, Gong X, Brink PR, Mathias RT, and White TW. Dominant cataracts result from incongruous mixing of wild-type lens connexins. J Cell Biol 161: 969-978, 2003.
26. Marziano NK, Casalotti SO, Portelli AE, Becker DL, and Forge A. Mutations in the gene for connexin 26 (GJB2) that cause hearing loss have a dominant negative effect on connexin 30. Hum Mol Genet 12: 805-812, 2003.
27. Maw MA, Allen-Powell DR, Goodey RJ, Stewart IA, Nancarrow DJ, Hayward NK, and Gardner RJ. The contribution of the DFNB1 locus to neurosensory deafness in a Caucasian population. Am J Hum Genet 57: 629-635, 1995.
28. Morell R, Kim H, Hood L, Goforth L, Friderici K, Fisher R, Van CG, Berlin C, Oddoux C, Ostrer H, Keats B, and Friedman T. Mutations in the connexin 26 gene (GJB2) among Ashkenazi Jews with nonsyndromic recessive deafness. N Engl J Med 339: 1500-1505, 1998.
29. Rabionet R, Lopez-Bigas N, Arbones ML, and Estivill X. Connexin mutations in hearing loss, dermatological and neurological disorders. Trends Mol Med 8: 205-212, 2002.
30. Saez JC, Connor JA, Spray DC, and Bennett MV. Hepatocyte gap junctions are permeable to the second messenger, inositol 1,4,5-trisphosphate, and to calcium ions. Proc Natl Acad Sci USA 86: 2708-2712, 1989.
31. Sohl G, Eiberger J, Jung YT, Kozak CA, and Willecke K. The mouse gap junction gene connexin29 is highly expressed in sciatic nerve and regulated during brain development. Biol Chem 382: 973-978, 2001.
32. Souter M and Forge A. Intercellular junctional maturation in the stria vascularis: possible association with onset and rise of endocochlear potential. Hear Res 119: 81-95, 1998.
33. Teubner B, Michel V, Pesch J, Lautermann J, Cohen-Salmon M, Sohl G, Jahnke K, Winterhager E, Herberhold C, Hardelin JP, Petit C, and Willecke K. Connexin30 (Gjb6)-deficiency causes severe hearing impairment and lack of endocochlear potential. Hum Mol Genet 12: 13-21, 2003.
34. Valiunas V, Manthey D, Vogel R, Willecke K, and Weingart R. Biophysical properties of mouse connexin30 gap junction channels studied in transfected human HeLa cells. J Physiol 519: 631-644, 1999.
35. Weber PA, Chang HC, Spaeth KE, Nitsche JM, and Nicholson BJ. The permeability of gap junction channels to probes of different size is dependent on connexin composition and permeant-pore affinities. Biophys J 87: 958-973, 2004.
36. White TW. Unique and redundant connexin contributions to lens development. Science 295: 319-320, 2002.
37. White TW and Bruzzone R. Intercellular communication in the eye: clarifying the need for connexin diversity. Brain Res Rev 32: 130-137, 2000.
38. Willecke K, Eiberger J, Degen J, Eckardt D, Romualdi A, Guldenagel M, Deutsch U, and Sohl G. Structural and functional diversity of connexin genes in the mouse and human genome. Biol Chem 383: 725-737, 2002.
39. Xia AP, Ikeda K, Katori Y, Oshima T, Kikuchi T, and Takasaka T. Expression of connexin 31 in the developing mouse cochlea. Neuroreport 11: 2449-2453, 2000.
40. Xia JH, Liu CY, Tang BS, Pan Q, Huang L, Dai HP, Zhang BR, Xie W, Hu DX, Zheng D, Shi XL, Wang DA, Xia K, Yu KP, Liao XD, Feng Y, Yang YF, Xiao JY, Xie DH, and Huang JZ. Mutations in the gene encoding gap junction protein beta-3 associated with autosomal dominant hearing impairment. Nat Genet 20: 370-373, 1998.