Preferential recognition of undisruptable dimers of inducible nitric oxide synthase by a monoclonal antibody directed against an N-terminal epitope

Journal of Immunology

Volumn 174, Issue 4, 2005, Pages 2314-2317

  Mazumdar, Tuhina ^a   Eissa, N Tony ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIMER; EPITOPE; INDUCIBLE NITRIC OXIDE SYNTHASE; MONOCLONAL ANTIBODY; OXYGENASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; DENATURATION; DIMERIZATION; ENZYME STRUCTURE; HUMAN; HUMAN CELL; MOLECULAR RECOGNITION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DOMAIN;

EID: 13544272028     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: 10.4049/jimmunol.174.4.2314     Document Type: Article

References (23)

1. Ignarro, L. J., G. M. Buga, K. S. Wood, R. E. Byrns, and G. Chaudhuri. 1987. Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide. Proc. Natl. Acad. Sci. USA 84:9265.
2. Xie, Q.-W., H. J. Cho, J. Calaycay, R. A. Mumford, K. M. Swiderek, T. D. Lee. A. Ding, T. Troso, and C. Nathan. 1992. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science 256:225.
3. Stuehr, D. J. 1999. Mammalian nitric oxide synthases. Biochim. Biophys. Acta. 1411:217.
4. Eissa, N. T., A. J. Strauss, C. M. Haggerty, E. K. Choo, S. C. Chu, and J. Moss. 1996. Alternative splicing of human inducible nitric-oxide synthase mRNA: tissue-specific regulation and induction by cytokines. J. Biol. Chem. 271:27184.
5. Geller, D. A., C. J. Lowenstein, R. A. Shapiro, A. K. Nussler, M. Di Silvio, S. C. Wang, D. K. Nakayama, R. L. Simmons, S. H. Snyder, and T. R. Billiar. 1993. Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes. Proc. Natl. Acad. Sci. USA 90:3491.
6. Ghosh, D. K., D. J. Stuehr. 1995. Macrophage NO synthase: characterization of isolated oxygenase and reductase domains reveals a head-to-head subunit interaction. Biochemistry 34:801.
7. Crane, B. R., A. S. Arvai, D. K. Ghosh, C. Wu, E. D. Getzoff, D. J. Stuehr, and J. A. Tainer. 1998. Structure of nitric oxide synthase oxygenase dimer with pterin and substrate. Science 279:2121.
8. Abu-Soud, H. M., and D. J. Stuehr. 1993. Nitric oxide synthases reveal a role for calmodulin in controlling electron transfer. Proc. Natl. Acad. Sci. USA 90:10769.
9. Cho, H. J., Q.-W. Xie, J. Calaycay, R. A. Mumford, K. M. Swiderek, T. D. Lee, and C. Nathan. 1992. Calmodulin is a subunit of nitric oxide synthase from macrophages. J. Exp. Med. 176:599.
10. Nathan, C. 1997. Inducible nitric oxide synthase: what difference does it make? J. Clin. Invest. 100:2417.
11. Guo, F. H., S. A. A. Comhair, S. Zheng, R. A. Dweik, N. T. Eissa, M. J. Thomassen, W. Calhoun, and S. C. Erzurum. 2000. Molecular mechanisms of increased nitric oxide (NO) in asthma: evidence for transcriptional and posttranslational regulation of NO synthesis. J. Immunol. 164:5970.
12. Eissa, N. T., J. Yuan, C. M. Haggerty, E. K. Choo, and J. Moss. 1998. Cloning and characterization of human inducible nitric oxide synthase splice variants: a domain, encoded by exons 8 and 9, is critical for dimerization. Proc. Natl. Acad. Sci. USA 95:7625.
13. Eissa, N. T., C. M. Haggerty, C. D. Palmer, W. Patton, and J. Moss. 2001. Identification of residues critical for enzymatic activity in the domain encoded by exons 8 and 9 of the human inducible nitric oxide synthase. Am. J. Respir. Cell Mol. Biol. 24:616.
14. Ghosh, D. K., M. B. Rashid, B. Crane, V. Taskar, M. Mast, M. Misukonis. J. B. Weinberg, and N. T. Eissa. 2001. Characterization of key residues, in the subdomain encoded by exons 8-9 of human inducible nitric oxide synthase: a critical role for Asp 280 in substrate binding and subunit interactions. Proc. Natl. Acad. Sci. USA 98:10392.
15. Raman, C. S., H. Li, P. Martasek, V. Kral, B. S. Masters, and T. L. Poulos. 1998. Crystal structure of constitutive endothelial nitric oxide synthase: a paradigm for pterin function involving a novel metal center. Cell 95:939.
16. Li, H., C. S. Raman, C. B. Glaser, E. Blasko, T. A. Young, J. F. Parkinson, M. Whitlow, and T. L. Poulos. 1999. Crystal structures of zinc-free and -bound heme domain of human inducible nitric-oxide synthase: implications for dimer stability and comparison with endothelial nitric-oxide synthase. J. Biol. Chem. 274:21276.
17. Kolodziejski, P. J., M. B. Rashid, and N. T. Eissa. Intracellular formation of novel "undisruptable" dimers of inducible nitric oxide synthase. Proc. Natl. Acad. Sci. USA 100:14263.
18. Musial, A., and N. T. Eissa. 2001. Inducible nitric oxide synthase is regulated by the proteasome degradation pathway. J. Biol. Chem. 276:24268.
19. Kolodziejski, P. J., A. Musial, J.-S. Koo, and N. T. Eissa. 2002. Ubiquitination of inducible nitric oxide synthase is required for its degradation. Proc. Natl. Acad. Sci. USA 99:12315.
20. Adam-Klages, S., D. Adam, K. Wiegmann, S. Strove, W. Kolanus, J. Schneider-Mergener, and M. Kränke. 1996. FAN, a novel WD-repeat protein, couples the p55 TNF-receptor to neutral sphingomyelinase. Cell 86:937.
21. Harlow, E., and D. Lane. 1999. Using Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
22. Klatt, P., K. Schmidt, D. Lehner, O. Glatter, H. P. Bächinger, and B. Mayer. 1995. Structural analysis of porcine brain nitric oxide synthase reveals a role for tetrahydrobiopterin and L-arginine in the formation of an SDS-resistant dimer. EMBO J. 14:3687.
23. Venema, R. C., H. Ju, R. Zou, J. W. Ryan, and V. J. Venema. 1997. Subunit interactions of endothelial nitric-oxide synthase: comparisons to the neuronal and inducible nitric-oxide synthase isoforms. J. Biol. Chem. 272:1276.