Less is better: Uncorrected versus pheopigment-corrected photometric chlorophyll-a estimation

Archiv fur Hydrobiologie

Volumn 162, Issue 1, 2005, Pages 111-120

  Stich, H B ^a   Brinker, A ^b  

^a Institut für Seenforschung   (Germany)

^b Fischereiforschungsstelle   (Germany)

Author keywords

Acidfication; Chlorophyll a; HPLC; Method; Pheophytin; Photometry

Indexed keywords

ACIDIFICATION; CHLOROPHYLL A; ENVIRONMENTAL MONITORING; LACUSTRINE ENVIRONMENT; LIQUID CHROMATOGRAPHY; METHODOLOGY;

EID: 13544269419     PISSN: 00039136     EISSN: None     Source Type: Journal    
DOI: 10.1127/0003-9136/2005/0162-0111     Document Type: Article

References (27)

1. ARAR, E. J. & COLLINS, G. B. (1997): Method 445: In Vitro determination of chlorophyll a and phaeophytin a in marine and freshwater algae by fluorescence. - National Exposure Research Laboratory Office of Research and Development, US Environmental Protection Agency, Cincinnati, OH, pp. 21.
2. DIN (1989): Concepts in the field of quality and statistics; structure and overall index. - DIN 55350, Deutsches Institut für Normung e. V., Beuth Verlag.
3. nd ed. - MacGraw-Hill, New York, pp. 476.
4. IRMER, U. (2001): Die neue EG-Wasserrahmenrichtlinie: Bewertung der chemischen und ökologischen Qualität von Oberflächengewä ssern - The new EC framework water directive: Assessment of the chemical and ecological status of surface waters. - Acta Hydrochim. Hydrobiol. 28: 7-14.
5. JACOBSON, T. R. (1982): Comparison of chlorophyll a measurements by fluorometric, spectrophotometric and high pressure liquid chromatographic methods in aquatic environments. - Arch. Hydrobiol. Spec. Iss. Advanc. Limnol. 16: 35-45.
6. JACOBSON, T. R. & RAI, H. (1990): Comparison of spectrophotometric, fluorometric and high performance liquid chromatography methods for the determination of chlorophyll a in aquatic samples: Effect of solvents and extraction procedure. - Internat. Rev. ges. Hydrobiol. 75: 207-217.
7. JEFFREY, S. W. & MANTOURA, R. F. C. (1997): Development of pigment methods for oceanography: SCOR supported working groups and objectives. - In: JEFFREY, S. W. MANTOURA, R. F. C. & WRIGHT, S. W. (eds): Phytoplankton pigments in oceanography. - Unesco Publishing, Paris, pp. 19-36.
8. JEFFREY, S. W., MANTOURA, R. F. C. & WRIGHT, S. W. (eds) (1997): Phytoplankton pigments in oceanography. - Unesco Publishing, Paris, pp. 661.
9. KOURIL, R., ILÍK, P., NAUS, J. & SCHOEFS, B. (1999): On the limits of applicability of spectrophotometric and spectrofluorometric methods for the determination of chlorophyll-a/b ratio. - Photosynthesis Res. 62: 107-116.
10. LIAAEN-JENSEN, S. (1978): Marine carotenoids. - In: SCHEUER, P. J. (ed.): Marine natural products. Chemical and biological perspectives. - Academic Press, New York-London, pp. 2-73.
11. MANTOURA, R. F. C., JEFFREY, S. W., LLEWELLYN, C. A., CLAUSTRE, H. & MORALES, C. E. (1997): Comparison between spectrophotometric, fluorometric and HPLC methods for chlorophyll analysis. - In: JEFFREY, S. W., MANTOURA, R. F. C. & WRIGHT, S. W. (eds): Phytoplankton pigments in oceanography. - UNESCO Publishing, Paris, pp. 361-380.
12. MARKER, A. F. H. (1972): The use of acetone and methanol in the estimation of chlorophyll in the presence of pheophytin. - Freshwat. Biol. 2: 361-385.
13. MEYNS, S., ILLI, R. & RIBI, B. (1994): Comparison of chlorophyll-a analysis by HPLC and spectrophotometry: Where do the differences come from? - Arch. Hydrobiol. 132: 129-139.
14. MORTENSEN, A. & SKIBSTEDT, L. H. (2000): Kinetics and mechanism of the primary steps of degradation of carotenoids by acid in homogeneous solution. - J. Agric. Food Chem. 48: 279-286.
15. NEVEUX, J., DELMAS, D., ROMANO, J. C., ALGARRA, P., IGNATIADES, L., HERBLAND, A., MORNAD, P., NECPA, A., BONIN, D., BARBE, J., SUKENIK, A. & BERMAN, T. (1990): Comparison of chlorophyll and phaeopigment determinations by spectrophotometric, fluorometric, spectrofluorometric and HPLC methods. - Mar. Microb. Food Webs 4: 217-238.
16. NUSCH, E. A. (1980): Comparison of different methods for chlorophyll and phaeopigment determination. - Arch. Hydrobiol. Spec. Iss. Advanc. Limnol. 14: 14-36.
17. RIEMANN, B. (1978): Carotenoid interference in the spectrophotometric determination of chlorophyll degradation products from natural populations of phytoplankton. - Limnol. Oceanogr. 23: 1059-1066.
18. _ (1980): A note on the use of methanol as an extraction solvent for chlorophyll determination. - Arch. Hydrobiol. Spec. Iss. Advanc. Limnol. 14: 70-78.
19. th ed. - Springer Verlag, Berlin, Heidelberg, New York, 552 pp.
20. SCHANZ, F. & RAI, H. (1988): Extract preparation and comparison of fluorometric, chromatographic (HPLC) and spectrophotometric determinations of chlorophyll-a. - Arch. Hydrobiol. 112: 533-539.
21. SCHMID, H. & STICH, H. B. (1995): HPLC-analysis of algal pigments: comparison of columns, column properties and eluents. - J. Appl. Phycol. 7: 487-494.
22. STRICKLAND, I. D. H. & PARSONS, T. R. (1968): A practical handbook of seawater analysis. - Fish. Res. Board Can. Bull. 167: 170-199.
23. UNESCO (1966): Determination of photosynthetic pigments in seawater. - UNESCO Monographs on Oceanographic Methodology, pp. 1-69.
24. VOLLENWEIDER, R. A. & KEREKES, J. (1980): The loading concept as a basis for controlling eutrophication philosophy and preliminary results of the OECD programme on eutrophication. - Prog. Water Technol. 12: 5-18.
25. WEBB, D. J., BURNISON, B. K., TRIMBEE, A. M. & PREPAS, E. E. (1992): Comparison of chlorophyll-a extractions with ethanol and dimethyl sulfoxide/acetone, and a concern about spectrophotometric phaeopigment correction. - Can. J. Fish. Aquat. Sci. 49: 2331-2336.
26. nd ed. - CBS College Publishing, 767 pp.
27. WRIGHT, S. W., JEFFREY, S. W. & MANTOURA, R. F. C. (1997): Evaluation of methods and solvents for pigment extraction. - In: JEFFREY, S. W., MANTOURA, R. F. C. & WRIGHT, S. W. (eds): Phytoplankton pigments in oceanography. - UNESCO Publishing, Paris, pp. 261-282.