On the limits of applicability of spectrophotometric and spectrofluorimetric methods for the determination of chlorophyll a/b ratio

Photosynthesis Research

Volumn 62, Issue 1, 1999, Pages 107-116

  Kouřil, Roman ^a   Ilík, Petr ^a   Nauš, Jan ^a   Schoefs, Benoit ^b  

^a PALACKÝ UNIVERSITY   (Czech Republic)

^b UNIVERSITY OF SOUTH BOHEMIA   (Czech Republic)

Author keywords

Chl a b ratio determination; Chlorophyll b; Chlorophyll concentration; Fluorescence; Greening; Protochlorophyll(ide); Spectrofluorimetry; Spectrophoto metry

Indexed keywords

EID: 0342545959     PISSN: 01668595     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006359213151     Document Type: Article

References (26)

1. Argyroudi-Akoyunoglou JH and Akoyunoglou G (1970) Photoinduced changes in the chlorophyll a to chlorophyll b ratio in young bean plants. Plant Physiol 46: 247-249
2. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol 24: 1-15
3. Bertrand M and Schoefs B (1997) Working with photosynthetic pigments: problems and precautions. In: Pessarakli M (ed) Handbook of Photosynthesis, pp 151-172. Marcel Dekker: New York/Basel/Hong Kong
4. Boardman NK and Thorne SW (1971) Sensitive fluorescence method for the determination of chlorophyll a / chlorophyll b ratios. Biochim Biophys Acta 253: 222-231
5. Brouers M and Michel-Wolwertz MD (1983) Estimation of protochlorophyll(ide) contents in plant extracts: Re-evaluation of the molar absorption coefficient of protochlorophyll(ide). Photosynth Res 4: 54-75
6. Burkey KO (1986) Chlorophyll-protein complex composition and photochemical activity in developing chloroplasts from greening barley seedlings. Photosynth Res 10: 37-49
7. Grover A and Mohanty P (1993) Leaf senescence - induced alterations in structure and function of higher plant chloroplasts In: Abrol YP, Mohanty P and Govindjee (eds) Photosynthesis: Photoreactions to Plant Productivity, pp 225-256. Kluwer Academic Publishers, Dordecht/Boston/London
8. Jäkel N (1981) Zur Fluorometrischen Bestimmung von Chlorophyll a und Chlorophyll b. Kulturpflanze 29: 417-423
9. Knox RS (1973) IV. Transfer of electronic excitation energy in condensed systems. In: Checcucci A and Weate RA (eds) Primary Molecular Events in Photobiology, pp 45-77. Elsevier, Amsterdam
10. Lichtenthaler HK (1987) Chlorophylls and carotenoids. Pigments of photosynthetic biomembranes. Meth Enzymol 148: 350-382
11. Mader P. Chlad F, Chladová J, Novák V, Sofrová D, Nauš J, Kohlová Švábová M and Makovec P (1980) Development of photosynthetic apparatus of etiolated seedlings of spring barley grown under various temperature, nitrogen and NaCl concentration in the nutrient solution. Photosynthetica 14 (2): 222-235
12. Meister A (1992) New fluorometric method for determination of chlorophyll a/b ratio. Photosynthetica 26: 533-539
13. Nauš J (1983) A simple model for evaluation of optical effects in the fluorescence spectrum of planparallel layer samples. I. Theory. II. Applications. Acta Univ Palacki Olom, Fac.rer.nat. 76, Physica XXII: 9-34
14. Nauš J, Klinkovský T, Ilík P and Cikánek D (1994) Model studies of chlorophyll fluorescence reabsorption at chloroplast level under different exciting light conditions. Photosynth Res 40: 67-74
15. Neveux J and Panouse M (1987) Spectrofluorometric determination of chlorophylls and pheophytins. Arch Hydrobiol 109 (4): 567-581
16. Ogawa T and Shibata K (1965) A sensitive method for determining chlorophyll b in plant extracts. Photochem Photobiol 4: 193-200
17. Porra RJ (1991) Recent advances and re-assessments in chlorophyll extraction and assay procedures for terrestrial, aquatic and marine organisms, including recalcitrant algae. In: Scheer H (ed) Chlorophylls, pp 31-57. CRC Press, Boca Raton, FL
18. Porra RJ, Thompson WA and Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: Verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975: 384-394
19. Schoefs B and Bertrand M (1997) Chlorophyll Biosynthesis. In: Pessarakli M (ed) Handbook of Photosynthesis, pp 49-70. Marcel Dekker: New York/Basel/Hong Kong
20. Schoefs B, Bertrand M and Lemoine Y (1998) Changes in the photosynthetic pigments in bean leaves during the first photoperiod of greening and the subsequent dark-phase. Comparison between old (10-d-old) leaves and young (2-d-old) leaves. Photosynth Res 57: 203-213
21. Shubin VV and Sabat SC (1990) A simple room temperature spectrofluorimetric method for determination of chlorophyll a/b ratio. Indian J Exp Biol 28: 87-90
22. Smith JHC and Benitez A (1955) Chlorophylls: Analysis in Plant Materials. In: Paech K and Tracey MV (eds) Modern Methods of Plant Analysis, Vol 4, pp 142-196. Springer-Verlag, Berlin
23. Šesták Z (1971) Determination of Chlorophylls a and b In: Šesták Z, Čatský J and Jarvis PG (eds) Plant Photosynthetic Production. Manual of Methods, pp 672-702. Dr W Junk NV Publishers, The Hague
24. Talbot MFJ and Sauer K (1997) Spectrofluorimetric method for the determination of large chlorophyll a/b ratios. Photosynth Res 53: 73-79
25. Weber G and Teale FWJ (1957) Determination of the absolute quantum yield of fluorescent solutions. Trans Faraday Soc 53: 646-655
26. Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144: 307-313