Functional analysis of two divalent metal-dependent regulatory genes dmdR1 and dmdR2 in Streptomyces coelicolor and proteome changes in deletion mutants

FEBS Journal

Volumn 272, Issue 3, 2005, Pages 725-735

  Flores, Francisco J ^a   Barreiro, Carlos ^b   Coque, Juan José R ^a,b   Martín, Juan F ^a,b  

^a UNIVERSITY OF LEÓN   (Spain)

^b Institute of Biotechnology of Leon   (Spain)

Author keywords

Iron metabolism; Proteome changes; Regulatory proteins; Streptomyces

Indexed keywords

BINDING PROTEIN; DIVALENT METAL DEPENDENT REGULATOR PROTEIN 1; DIVALENT METAL DEPENDENT REGULATOR PROTEIN 2; DNA BINDING PROTEIN; FERRITIN; FRUCTOSE BISPHOSPHATE ALDOLASE; GAS VESICLE PROTEIN; GLUTAMATE DEHYDROGENASE; GLUTAMIC ACID TRANSFER RNA GLUTAMINE AMIDOTRANSFERASE; IRON; MALATE DEHYDROGENASE; PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN; PROTEOME; REGULATOR PROTEIN; SUPEROXIDE DISMUTASE; UNCLASSIFIED DRUG; ZINC DEPENDENT DEHYDROGENASE;

ARTICLE; COSMID; DELETION MUTANT; DIMERIZATION; DMDR1 GENE; DMDR2 GENE; GENE DISRUPTION; GENETIC COMPLEMENTATION; IRON METABOLISM; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METAL BINDING; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; REGULATOR GENE; SEQUENCE HOMOLOGY; STREPTOMYCES COELICOLOR; WESTERN BLOTTING; WILD TYPE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CATIONS, DIVALENT; DNA PRIMERS; GENE DELETION; GENES, BACTERIAL; GENES, REGULATOR; GENETIC COMPLEMENTATION TEST; MOLECULAR SEQUENCE DATA; MUTATION; PROTEOME; SEQUENCE HOMOLOGY, AMINO ACID; STREPTOMYCES COELICOLOR;

ACTINOBACTERIA (CLASS); POSIBACTERIA; STREPTOMYCES; STREPTOMYCES COELICOLOR; UNCULTURED ACTINOMYCETE;

EID: 13444293049     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2004.04509.x     Document Type: Article

References (29)

1. Flores FJ & Martín JF (2004) Iron-regulatory proteins DmdR1 and DmdR2 of Streptomyces coelicolor form two different DNA-protein complexes with iron boxes. Biochem J 379, 497-503.
2. De Lorenzo V, Giovannini F, Herrero M & Neilands JB (1988) Metal ion regulation of gene expression. Fur repressor-operator interaction at the promoter region of the aerobactin system of pCo1V-K30. J Mol Biol 203, 875-884.
3. De Lorenzo V, Herrero M, Giovannini F & Neilands JB (1988) Fur (ferric uptake regulation) protein and CAP (catabolite-activator protein) modulate transcription of the fur gene in Escherichia coli. Eur J Biochem 173, 537-546.
4. Schmitt MP & Holmes RK (1991) Iron-dependent regulation of diphtheria toxin and siderophore expression by the cloned Corynebacterium diphtheriae repressor gene dtxR in C. diphtheriae C7 strains. Infect Immun 59, 1899-1904.
5. Schmitt MP, Twiddy EM & Holmes RK (1992) Purification and characterization of the diphtheria toxin repressor. Proc Natl Acad Sci USA 89, 7576-7580.
6. Tao X & Murphy JR (1992) Binding of the metallo-regulatory protein DtxR to the diphteria tox operator requires a divalent heavy metal ion and protects the palindromic sequence from Dnase I digestion. J Biol Chem 267, 21761-21764.
7. Oguiza JA, Marcos AT, Malumbres M & Martín JF (1996) Multiple sigma factor genes in Brevibacterium lactofermentum: characterization of sigA and sigB. J Bacteriol 178, 550-553.
8. Oguiza JA, Marcos AT, Malumbres M & Martín JF (1996) The galE gene encoding the UDP-galactose 4-epimerase of Brevibacterium lactofermentum is coupled transcriptionally to the dmdR gene. Gene 177, 103-107.
9. Rincón J (2000) Análisis de la región genómica que codifica los factores sigma principales RofA y RofB de Rhodococcus fascians D188. PhD Thesis. University of León, Spain.
10. Doukhan L, Predich M, Nair G, Dussurget O, Mandic-Mulec I, Cole ST, Smith DR & Smith I (1995) Genomic organization of the mycobacterial sigma gene cluster. Gene 165, 67-70.
11. Martin JF, Gutiérrez S & Aparicio JF (2000) Secondary metabolites. In Encyclopedia of Microbiology, Vol. 4, 2nd edn (Lederberg J, ed.), pp. 213-237. Academic Press, San Diego.
12. Bentley SD, Chater KF, Cerdeno-Tarraga AM, Challis GL, Thomson NR, James KD, Harris DE, Quail MA, Kieser H, Harper D, et al. (2002) Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417, 141-147.
13. Günter-Seeboth K & Schupp T (1995) Cloning and sequence analysis of the Corynebacterium diphtheriae dtxR homologue from Streptomyces lividans and S. pilosus encoding a putative iron repressor protein. Gene 166, 117-119.
14. Redenbach M, Kieser HM, Denapaite D, Eichner A, Cullum J, Kinashi H & Hopwood DA (1996) A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome. Mol Microbiol 21, 77-96.
15. Pena MM & Bullerjahn GS (1995) The DpsA protein of Synechococcus sp. strain PCC7942 is a DNA-binding hemoprotein. Linkage of the Dps and bacterioferritin protein families. J Biol Chem 270, 22478-22482.
16. Loprasert S, Whangsuk W, Sallabhan R & Mongkolsuk S (2004) DpsA protects the human pathogen Burkholderia pseudomallei against organic peroxide. Arch Microbiol 182, 96-101.
17. Bao K, Zhou X, Kieser T & Deng Z (1997) pHZ1351, a Broad Host-Range Plasmid Vector Useful for Gene Cloning and for Gene Replacement in Streptomyces hygroscopicus KMP3. XI International Symposium on Biology of Actinomycetes (ISBA) '97, Beijing, China. Abstract 4P15.
18. Boyd J, Hall KM & Murphy JR (1992) DNA sequencing and characterization of dtxR alleles from Corynebacterium diphtheriae PW8(-), 1030(-), and C7hm723(-). J Bacteriol 174, 1268-1272.
19. Boyd J, Oza MN & Murphy JR (1990) Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae. Proc Natl Acad Sci USA 87, 5968-5972.
20. Hill PJ, Cockayne A, Landers P, Morrisey JA, Sims CM & Williams P (1998) SirR, a novel iron-dependent repressor in Staphylococus epidermidis. Infect Immun 66, 4123-4129.
21. Schiering N, Tao X, Zeng H-Y, Murphy JR, Petsko GA & Ringe D (1995) Structures of the apo- and the metal ion-activated forms of the diphtheria tox repressor from Corynebacterium diphtheriae. Proc Natl Acad Sci USA 92, 9843-9850.
22. Qiu X, Verlinde CLMJ, Zhang S, Schmitt MP, Holmes RK & Hol WGJ (1995) Three-dimensional structure of the diphtheria toxin repressor in complex with divalent cation co-repressors. Structure 3, 87-100.
23. Wang Z, Schmitt MS & Holmes RK (1994) Characterization of mutations that inactivate the diphtheria toxin repressor gene (dtxR). Infect Immun 62, 1600-1608.
24. Flores JF, Rincón J & Martin JF (2003) Characterization of the iron-regulated desA promoter of Streptomyces pilosus as a system for controlled gene expression in actinomycetes. Microbial Cell Factories 2, 5.
25. Morrisey JA, Cockayne A, Brummell K & Williams P (2004) The staphylococcal ferritins are differentially regulated in response to iron and manganese and via PerR and Fur. Infect Immun 72, 972-979.
26. Kieser T, Bibb MJ, Buttner MJ, Chater KF & Hopwood DA (2000) Practical Streptomyces Genetics. The John Innes Foundation, Norwich, UK.
27. Sambrook J & Russell DW (2001) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
28. Görg A, Obermaier C, Boguth G, Harder A, Shceibe B, Wildgruber R & Weiss W (2000) The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21, 1037-1053.
29. Perkins DN, Pappin DJ, Creasy DM & Cottrell JS (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20, 3551-3567.