Efficient micro-recovery and guanidination of peptides directly from MALDI target spots

BioTechniques

Volumn 38, Issue 2, 2005, Pages 301-304

  Cockrill, Steven L ^a   Foster, Kelly L ^a   Wildsmith, Justin ^a   Goodrich, April R ^a   Dapron, John G ^a   Hassell, Tom C ^a   Kappel, William K ^a   Scott, Graham B I ^a  

^a Sigma Aldrich Co   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DESORPTION; ENZYMES; GELS; IONIZATION; LASERS; SAMPLING; SOLUTIONS;

ENZYMATIC TREATMENT; IN-GEL DIGESTS; PROTEIN IDENTIFICATION; TARGET SPOTS;

POLYPEPTIDES;

PEPTIDE;

AMINO ACID SEQUENCE; AQUEOUS SOLUTION; ARTICLE; CHEMICAL REACTION; DERIVATIZATION; GEL; GUANIDINATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PROTEIN ANALYSIS; PROTEIN PROCESSING; PROTEOMICS; QUANTITATIVE ANALYSIS; SEQUENCE ANALYSIS;

EID: 13444263258     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/05382PT02     Document Type: Article

References (23)

1. Krause, E., H. Wenschuh, and P.R. Jungblut. 1999. The dominance of arginine-containing peptides in MALDI-derived tryptic mass fingerprints of proteins. Anal. Chem. 71:4160-4165.
2. Baumgart, S., Y. Lindner, R. Kühne, A. Oberemm, H. Wenschuh, and E. Krause. 2004. The contributions of specific amino acid side chains to signal intensities of peptides in matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 18:863-868.
3. Zhu, Y.F., K.L. Lee, K. Tang, S.L. Allman, N.I. Taranenko, and C.H. Chen. 1995. Revisit of MALDI for small proteins. Rapid Commun. Mass Spectrom. 9:1315-1320.
4. Wenschuh, H., P. Halada, S. Lamer, P. Jungblut, and E. Krause. 1998. The ease of peptide detection by matrix-assisted laser desorption/ionization mass spectrometry: the effect of secondary structure on signal intensity. Rapid Commun. Mass Spectrom. 12:115-119.
5. Kratzer, R., C. Eckerskorn, M. Karas, and F. Lottspeich. 1998 Suppression effects in enzymatic peptide ladder sequencing using ultraviolet-matrix assisted laser desorption/ionization-mass spectrometry. Electrophoresis 19:1910-1919.
6. Beardsley, R.L., J.A. Karty, and J.P. Reilly. 2000. Enhancing the intensities of lysine-terminated tryptic peptide ions in matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 14:2147-2153.
7. Hale, J.E., J.P. Butler, M.D. Knierman, and G.W. Becker. 2000. Increased sensitivity of tryptic peptide detection by MALDI-TOF mass spectrometry is achieved by conversion of lysine to homoarginine. Anal. Biochem. 287:110-117.
8. Brancia, F.L., S.G. Oliver, and S.J. Gaskell. 2000. Improved matrix-assisted laser desorption/ionization mass spectrometric analysis of tryptic hydrolysates of proteins following guanidination of lysine-containing peptides. Rapid Commun. Mass Spectrom. 14:2070-2073.
9. Beardsley, R.L. and J.P. Reilly. 2002. Optimization of guanidination procedures for MALDI mass mapping. Anal. Chem. 74:1884-1890.
10. Thevis, M., R.R. Ogorzalek Loo, and J.A. Loo. 2003. In-gel derivatization of proteins for cysteine-specific cleavages and their analysis by mass spectrometry. J. Proteome Res. 2:163-172.
11. Fenaille, F., F. Morgan, V. Parisod, J.C. Tabet, and P.A. Guy. 2004. Solid-state glycation of β-lactoglobulin by lactose and galactose: localization of the modified amino acids using mass spectrometric techniques. J. Mass. Spectrom. 39:16-28.
12. l2. Cagney, C. and A. Emili. 2002. De novo peptide sequencing and quantitative profiling of complex protein mixtures using mass-coded abundance tagging. Nat. Biotechnol. 20:163-170.
13. Lemmel, C., S. Weik, U. Eberle, J. Dengjel, T. Kratt, H.-D. Becker, H.-G. Rammensee, and S. Stevanovic. 2004. Differential quantitative analysis of MHC ligands by mass spectrometry using stable isotope labeling. Nat. Biotechnol. 22:450-454.
14. Brancia, F.L., H. Montgomery, K. Tanaka, and S. Kumashiro. 2004. Guanidino labeling derivatization strategy for global characterization of peptide mixtures by liquid chromatography matrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem. 76:2748-2755.
15. Keough, T., M.P. Lacey, and R.S. Youngquist. 2002. Solid-phase derivatization of tryptic peptides for rapid protein identification by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 16:1003-1015.
16. Bonetto, V., A.C. Bergman, H. Jörnvall, and R. Sillard. 1997. C-terminal sequence analysis of peptides using carboxypeptidases and mass spectrometry after derivatization of Lys and Cys residues. Anal. Chem. 69:1315-1319.
17. Brancia, F.L., M.E. Openshaw, and S. Kumashiro. 2002. Investigation of the electrospray response of lysine-, arginine-, and homoarginine-terminal peptide mixtures by liquid chromatography/mass spectrometry. Rapid Commun. Mass Spectrom. 16:2255-2259.
18. Cerpa-Poljak, A., A. Jenkins. and M.W. Duncan. 1995. Recovery of peptides and proteins following matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 9:233-239.
19. Küster, B., T.J.P. Naven, and D.J. Harvey. 1996. Rapid approach for sequencing neutral oligosaccharides by exoglycosidase digestion and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J. Mass Spectrom. 31:1131-1140.
20. Terry, D.E., E. Umstot, and D.M. Desiderio. 2004. Optimized sample-processing time and peptide recovery for the muss spectrometric analysis of protein digests. J. Am. Soc. Mass Spectrom. 15:784-794.
21. Rosenfeld, J.M. 2003. Derivatization in the current practice of analytical chemistry. Trends Anal. Chem. 22:785-798.
22. Zhen, Y., N. Xu, B. Richardson, R. Becklin, J.R. Savage, K. Blake, and J.M. Peltier. 2004. Development of an LC-MALDI method for the analysis of protein complexes. J. Am. Soc. Mass Spectrom. 15:803-822.
23. Bodnar, W.M., R.K. Blackburn, J.M. Krise, and M.A. Moseley. 2003 Exploiting the complementary nature of LC/MALDI/MS/MS and LC/ESI/MS/MS for increased proteome coverage. J. Am. Soc. Mass Spectrom. 14:971-979.