Studies of the Interaction of Escherichia coli YjeQ with the Ribosome In Vitro

Journal of Bacteriology

Volumn 186, Issue 5, 2004, Pages 1381-1387

  Daigle, Denis M ^a   Brown, Eric D ^a  

^a MCMASTER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; GUANINE NUCLEOTIDE; GUANOSINE PHOSPHATE; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; GUANYLYLIMIDODIPHOSPHATE; NUCLEOTIDE BINDING PROTEIN; PROTEIN YJEQ; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; ENZYME ACTIVITY; ESCHERICHIA COLI; HYDROLYSIS; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; RIBOSOME; STEADY STATE; STOICHIOMETRY;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GTP PHOSPHOHYDROLASES; MUTATION; RIBOSOMAL PROTEINS; RIBOSOMES;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 1342346614     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.5.1381-1387.2004     Document Type: Article

References (17)

1. An, G., B. R. Glick, J. D. Friesen, and M. C. Ganoza. 1980. Identification and quantitation of elongation factor EF-P in Escherichia coli cell-free extracts. Can. J. Biochem. 97:23-28.
2. Aoki, H., K. Dekany, S.-L. Adams, and M. C. Ganoza. 1997. The gene encoding the elongation factor P protein is essential for viability and is required for protein synthesis. J. Biol. Chem. 272:32254-32259.
3. Arcus, V. 2002. OB-fold domains: a snapshot of the evolution of sequence, structure and function. Curr. Opin. Struct. Biol. 12:794-801.
4. Arigoni, F., F. Talabot, M. Peitsch, M. D. Edgerton, E. Meldrum, E. Allet, R. Fish, T. Jamotte, M. L. Curchod, and H. Loferer. 1998. A genome-based approach for the identification of essential bacterial genes. Nat. Biotechnol. 16:851-856.
5. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1994. Current protocols in molecular biology, vol. 2, chapter 11, section 11.12-11.13. John Wiley & Sons Inc., Boston, Mass.
6. Bourne, H. R., D. A, Sanders, and F. McCormick. 1990. The GTPase super-family: a conserved switch for diverse cell functions. Nature 348:125-132.
7. Bourne, H. R., D. A. Sanders, and F. McCormick. 1991. The GTPase super-family: conserved structure and molecular mechanism. Nature 349:117-127.
8. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 34:248-254.
9. Daigle, D. M., L. Rossi, A. M. Berghuis, L. Aravind, E. V. Koonin, and E. D. Brown. 2002. YjeQ, an essential, conserved, uncharacterized protein from Escherichia coli, is an unusual GTPase with circularly permuted G-motifs and marked burst kinetics. Biochemistry 41:11109-11117.
10. Gill, S. C., and P. H. von Hippel. 1989. Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182:319-326.
11. Hesterkamp, T., E. Deuerling, and B. Bukau. 1997. The amino-terminal 118 amino acids of Escherichia coli trigger factor constitute a domain that is necessary and sufficient for binding to ribosomes. J. Biol. Chem. 272:21865-21871.
12. Inoue, K., J. Alsina, J. Chen, and M. Inouye. 2003. Suppression of defective ribosome assembly in a rbfA deletion mutant by overexpression of Era, an essential GTPase in Escherichia coli. Mol. Microbiol. 48:1005-1016.
13. Kung, H. F., C. Spears, T. Schulz, and H. Weissbach. 1974. Studies on the in vitro synthesis of beta-galactosidase: necessary components in the ribosomal wash. Arch. Biochem. Biophys. 162:578-584.
14. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
15. Rodnina, M. V., H. Stark, A. Savelsbergh, H.-J. Wieden, N. B. Matassova, F. Peske, T. Daviter, C. O. Gualerzi, and W. Wintermeyer. 2000. GTPases: mechanisms and functions of translation factors on the ribosome. Biol. Chem. 381:377-387.
16. Spedding, G. 1990. Isolation and analysis of ribosomes from prokaryotes, eukaryotes and organelles, p. 5-7. In G. Spedding (ed.), Ribosomes and protein synthesis: a practical approach. IRL Press, New York, N.Y.
17. Sprinzl, M., S. Brock, Y. Huang, P. Milovnik, M. Nanninga, M. Nesper-Brock, H. Rutthard, and K. Szkaradkiewicz. 2000. Regulation of GTPases in the bacterial translation machinery. Biol. Chem. 381:367-375.