Identification of residues controlling transport through the yeast aquaglyceroporin Fps1 using a genetic screen

European Journal of Biochemistry

Volumn 271, Issue 4, 2004, Pages 771-779

  Karlgren, Sara ^a   Filipsson, Caroline ^a   Mullins, Jonathan G L ^b   Bill, Roslyn M ^a,c   Tamás, Markus J ^a   Hohmann, Stefan ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

^b SWANSEA UNIVERSITY   (United Kingdom)

^c ASTON UNIVERSITY   (United Kingdom)

Author keywords

Aquaglyceroporin; Channel; Genetic screen; Glycerol; Osmoregulation

Indexed keywords

AQUAGLYCEROPORIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; GENE IDENTIFICATION; GENE LOCATION; GENE MUTATION; GENETIC SCREENING; NONHUMAN; OSMOREGULATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS REGULATION; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; STRUCTURE ANALYSIS; YEAST;

AMINO ACID SEQUENCE; AQUAPORINS; BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; GENES, FUNGAL; GLYCEROL; MEMBRANE PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PHENOTYPE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSFORMATION, GENETIC;

SACCHAROMYCES CEREVISIAE;

EID: 1342322689     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2004.03980.x     Document Type: Article

References (32)

1. Preston, G.M., Carroll, T.P., Guggino, W.B. & Agre, P. (1992) Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 256, 385-387.
2. Borgnia, M., Nielsen, S., Engel, A. & Agre, P. (1999) Cellular and molecular biology of the aquaporin water channels. Annu. Rev. Biochem. 68, 425-458.
3. Hohmann, S., Nielsen, S. & Agre, P. (2001) Aquaporins. Academic Press, San Diego, CA.
4. Deen, P.M., Marr, N., Kamsteeg, E.J. & van Balkom, B.W. (2000) Nephrogenic diabetes insipidus. Curr. Opin. Nephrol. Hypertens. 9, 591-595.
5. Deen, P.M.T. & Brown, D. (2001) Trafficking of native and mutant mammalian MIP proteins. In Aquaporins (Hohmann, S., Nielsen, S. & Agre, P., eds), pp. 235-277. Academic Press, San Diego, CA.
6. Chrispeels, M.J., Morillon, R., Maurel, C., Gerbeau, P., Kjellbom, P. & Johansson, I. (2001) Aquaporins in plants: structure, function, regulation, and role in plant water relations. In Aquaporins (Hohmann, S., Nielsen, S. & Agre, P., eds), pp. 277-334. Academic Press, San Diegi, CA.
7. Tournaire-Roux, C., Sutka, M., Javot, H., Gout, E., Gerbeau, P., Luu, D.T., Bligny, R. & Maurel, C. (2003) Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins. Nature 425, 393-397.
8. Hohmann, S., Bill, R.M., Kayingo, I. & Prior, B.A. (2000) Microbial MIP channels. Trends Microbiol. 8, 33-38.
9. Tanghe, A., Van Dijck, P., Dumortier, F., Teunissen, A., Hohmann, S. & Thevelein, J.M. (2002) Aquaporin expression correlates with freeze tolerance in baker's yeast, and overexpression improves freeze tolerance in industrial strains. Appl. Environ. Microbiol. 68, 5981-5989.
10. Oliveira, R., Lages, F., Silva-Graca, M. & Lucas, C. (2003) Fps1p channel is the mediator of the major part of glycerol passive diffusion in Saccharomyces cerevisiae: artefacts and re-definitions. Biochim. Biophys. Acta. 1613, 57-71.
11. Hohmann, S. (2002) Osmotic stress signaling and osmoadaptation in yeasts. Microbiol. Mol. Biol. Rev. 66, 300-372.
12. Luyten, K., Albertyn, J., Skibbe, F., Prior, B.A., Ramos, J., Thevelein, J.M. & Hohmann, S. (1995) Fps1, a yeast member of the MIP-family of channel proteins, is a facilitator for glycerol uptake and efflux and it is inactive under osmotic stress. EMBO J. 14, 1360-1371.
13. Tamás, M.J., Luyten, K., Sutherland, F.C.W., Hernandez, A., Albertyn, J., Valadi, H., Li, H., Prior, B.A., Kilian, S.G., Ramos, J., Gustafsson, L., Thevelein, J.M. & Hohmann, S. (1999) Fps1p controls the accumulation and release of the compatible solute glycerol in yeast osmoregulation. Mol. Microbiol. 31, 1087-1104.
14. Philips, J. & Herskowitz, I. (1997) Osmotic balance regulates cell fusion during mating in Saccharomyces cerevisiae. J. Cell Biol. 138, 961-974.
15. Tamás, M.J., Karlgren, S., Bill, R.M., Hedfalk, K., Allegri, L., Ferreira, M., Thevelein, J.M., Rydstrom, J., Mullins, J.G. & Hohmann, S. (2003) A short regulatory domain restricts glycerol transport through yeast Fps1p. J. Biol. Chem. 278, 6337-6345.
16. Stahlberg, H., Heymann, B., Mitsuoka, K., Fuyijoshi, Y. & Engel, A. (2001) The aquaporin superfamily: structure and function. In Aquaporins (Hohmann, S., Nielsen, S. & Agre, P., eds), pp. 39-119. Academic Press, San Diego, CA.
17. Fu, D., Libson, A., Miercke, L.J., Weitzman, C., Nollert, P., Krucinski, J. & Stroud, R.M. (2000) Structure of a glycerolconducting channel and the basis for its selectivity. Science 290, 481-486.
18. Sui, H., Han, B.G., Lee, J.K., Walian, P. & Jap, B.K. (2001) Structural basis of water-specific transport through the AQP1 water channel. Nature 414, 872-878.
19. de Groot, B.L. & Grubmuller, H. (2001) Water permeation across biological membranes: mechanism and dynamics of aquaporin-1 and GlpF. Science 294, 2353-2357.
20. Zeuthen, T. (2001) How water molecules pass through aquaporins. Trends Biochem. Sci. 26, 77-79.
21. de Groot, B.L., Frigato, T., Helms, V. & Grubmuller, H. (2003) The mechanism of proton exclusion in the aquaporin-1 water channel. J. Mol. Biol. 333, 279-293.
22. Fujiyoshi, Y., Mitsuoka, K., de Groot, B.L., Philippsen, A., Grubmuller, H., Agre, P. & Engel, A. (2002) Structure and function of water channels. Curr. Opin. Struct. Biol. 12, 509-515.
23. Hansen, M., Kun, J.F., Schulte, J.E. & Beitz, E. (2002) A single, bi-functional aquaglyceroporin in blood-stage Plasmodium falciparum malaria parasites. J. Biol. Chem. 277, 4874-4882.
24. Bill, R.M., Hedfalk, K., Karlgren, S., Mullins, J.G., Rydstrom, J. & Hohmann, S. (2001) Analysis of the pore of the unusual major intrinsic protein channel, yeast Fps1p. J. Biol. Chem. 276, 36543-36549.
25. Reference withdrawn.
26. Ansell, R., Granath, K., Hohmann, S., Thevelein, J. & Adler, L. (1997) The two isoenzymes for yeast NAD-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2, have distinct roles in osmoadaption and redox regulation. EMBO J. 16, 2179-2187.
27. Thomas, B.J. & Rothstein, R.J. (1989) Elevated recombination rates in transcriptionally active DNA. Cell. 56, 619-630.
28. Sherman, F., Fink, G.R. & Hicks, J.B. (1983) Methods in Yeast Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. Gietz, D., St Jean, A., Woods, R.A. & Schiestl, R.H. (1992) Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 20, 1425.
30. Reference withdrawn.
31. Koradi, R., Billeter, M. & Wuthrich, K. (1996) MOLMOL: a program for display and analysis of macromolecular structures. J. Mol. Graph. 14 (51-5), 29-32.
32. Murata, K., Mitsuoka, K., Hirai, T., Walz, T., Agre, P., Heymann, J.B., Engel, A. & Fujiyoshi, Y. (2000) Structural determinants of water permeation through aquaporin-1. Nature 407, 599-605.