Systematic analysis, by the yeast two-hybrid, of protein interaction between components of the type II secretory machinery of Erwinia chrysanthemi

Research in Microbiology

Volumn 155, Issue 2, 2004, Pages 71-75

  Douet, Vanessa ^a   Loiseau, Laurent ^a   Barras, Frédéric ^a   Py, Béatrice ^a  

^a CNRS   (France)

Author keywords

Erwinia chrysanthemi; General secretory pathway; Out proteins; Protein interaction; Yeast two hybrid system

Indexed keywords

MEMBRANE PROTEIN; SECRETIN;

ARTICLE; CYTOPLASM; ENZYME RELEASE; ERWINIA; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; TWO HYBRID SYSTEM;

ERWINIA; ERWINIA CHRYSANTHEMI; NEGIBACTERIA;

EID: 1342302802     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.resmic.2003.10.001     Document Type: Article

References (28)

1. Ball G., Chapon-Herve V., Bleves S., Michel G., Bally M. Assembly of XcpR in the cytoplasmic membrane is required for extracellular protein secretion in Pseudomonas aeruginosa. J. Bacteriol. 181:1999;382-388.
2. Durand E., Bernadac A., Ball G., Lazdunski A., Sturgis J.N., Filloux A. Type II protein secretion in Pseudomonas aeruginosa: The pseudopilus is a multifibrillar and adhesive structure. J. Bacteriol. 185:2003;2749-2758.
3. Golemis E.A., Gyuris J., Brent R. Interaction trap/two-hybrid system to identify interacting proteins. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Current Protocols in Molecular Biology. 1994;Wiley, New York. unit 13.14.
4. He S.Y., Lindeberg M., Chatterjee A.K., Collmer A. Cloned Erwinia chrysanthemi out genes enable Escherichia coli to selectively secrete a diverse family of heterologous proteins to its milieu. Proc. Natl. Acad. Sci. USA. 88:1991;1079-1083.
5. He S.Y., Schoedel C., Chatterjee A.K., Collmer A. Extracellular secretion of pectate lyase by the Erwinia chrysanthemi Out pathway is dependent upon Sec-mediated export across the inner membrane. J. Bacteriol. 173:1991;4310-4317.
6. Hobbs M., Mattick J.S. Common components in the assembly of type IV fimbriae, DNA transfer systems, filamentous phage and protein-secretion apparatus: A general system for the formation of surface-associated protein complexes. Mol. Microbiol. 10:1993;233-243.
7. Hu N.T., Leu W.M., Lee M.S., Chen A., Chen S.C., Song Y.L., Chen L.Y. XpsG, the major pseudopilin in Xanthomonas campestris pv. campestris, forms a pilus-like structure between cytoplasmic and outer membranes. Biochem J. 365:2002;205-211.
8. Kagami Y., Ratliff M., Surber M., Martinez A., Nunn D.N. Type II protein secretion by Pseudomonas aeruginosa: Genetic suppression of a conditional mutation in the pilin-like component XcpT by the cytoplasmic component XcpR. Mol. Microbiol. 27:1998;221-233.
9. Lee H.M., Tyan S.W., Leu W.M., Chen L.Y., Chen D.C., Hu N.T. Involvement of the XpsN protein in formation of the XpsL-xpsM complex in Xanthomonas campestris pv. campestris type II secretion apparatus. J. Bacteriol. 183:2001;528-535.
10. Lee H.M., Wang K.C., Liu Y.L., Yew H.Y., Chen L.Y., Leu W.M., Chen D.C., Hu N.T. Association of the cytoplasmic membrane protein XpsN with the outer membrane protein XpsD in the type II protein secretion apparatus of Xanthomonas campestris pv. campestris. J. Bacteriol. 182:2000;1549-1557.
11. Lindeberg M., Salmond G.P., Collmer A. Complementation of deletion mutations in a cloned functional cluster of Erwinia chrysanthemi out genes with Erwinia carotovora out homologues reveals OutC and OutD as candidate gatekeepers of species-specific secretion of proteins via the type II pathway. Mol. Microbiol. 20:1996;175-190.
12. Lu H.M., Motley S.T., Lory S. Interactions of the components of the general secretion pathway: Role of Pseudomonas aeruginosa type IV pilin subunits in complex formation and extracellular protein secretion. Mol. Microbiol. 25:1997;247-259.
13. Possot O.M., Vignon G., Bomchil N., Ebel F., Pugsley A.P. Multiple interactions between pullulanase secreton components involved in stabilization and cytoplasmic membrane association of PulE. J. Bacteriol. 182:2000;2142-2152.
14. Pugsley A.P., Poquet I., Kornacker M.G. Two distinct steps in pullulanase secretion by Escherichia coli K12. Mol. Microbiol. 5:1991;865-873.
15. Py B., Loiseau L., Barras F. An inner membrane platform in the type II secretion machinery of Gram-negative bacteria. EMBO Rep. 2:2001;244-248.
16. Py B., Loiseau L., Barras F. Assembly of the type II secretion machinery of Erwinia chrysanthemi: Direct interaction and associated conformational change between OutE, the putative ATP-binding component and the membrane protein OutL. J. Mol. Biol. 289:1999;659-670.
17. Reeves P.J., Douglas P., Salmond G.P. Beta-Lactamase topology probe analysis of the OutO NMePhe peptidase, and six other Out protein components of the Erwinia carotovora general secretion pathway apparatus. Mol. Microbiol. 12:1994;445-457.
18. Sandkvist M. Biology of type II secretion. Mol. Microbiol. 40:2001;271-283.
19. Sandkvist M., Hough L.P., Bagdasarian M.M., Bagdasarian M. Direct interaction of the EpsL and EpsM proteins of the general secretion apparatus in Vibrio cholerae. J. Bacteriol. 181:1999;3129-3135.
20. Sandkvist M., Bagdasarian M., Howard S.P., DiRita V.J. Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae. EMBO J. 14:1995;1664-1673.
21. Sauvonnet N., Vignon G., Pugsley A.P., Gounon P. Pilus formation and protein secretion by the same machinery in Escherichia coli. EMBO J. 19:2000;2221-2228.
22. Shevchik V.E., Robert-Baudouy J., Condemine G. Specific interaction between OutD, an Erwinia chrysanthemi outer membrane protein of the general secretory pathway, and secreted proteins. EMBO J. 16:1997;3007-3016.
23. Strom M.S., Nunn D.N., Lory S. A single bifunctional enzyme, PilD, catalyzes cleavage and N -methylation of proteins belonging to the type IV pilin family. Proc. Natl. Acad. Sci. USA. 90:1993;2404-2408.
24. Thanassi D.G. Ushers and secretins: Channels for the secretion of folded proteins across the bacterial outer membrane. J. Mol. Microbiol. Biotechnol. 4:2002;11-20.
25. Thomas J.D., Reeves P.J., Salmond G.P. The general secretion pathway of Erwinia carotovora subsp. carotovora: Analysis of the membrane topology of OutC and OutF. Microbiology. 143:1997;713-720.
26. Tsai R.T., Leu W.M., Chen L.Y., Hu N.T. A reversibly dissociable ternary complex formed by XpsL, XpsM and XpsN of the Xanthomonas campestris pv. campestris type II secretion apparatus. Biochem. J. 367:2002;865-871.
27. Vignon G., Kohler R., Larquet E., Giroux S., Prevost M.C., Roux P., Pugsley A.P. Type IV-like pili formed by the type II secreton: Specificity, composition, bundling, polar localization, and surface presentation of peptides. J. Bacteriol. 185:2003;3416-3428.
28. Voulhoux R., Ball G., Ize B., Vasil M.L., Lazdunski A., Wu L.F., Filloux A. Involvement of the twin-arginine translocation system in protein secretion via the type II pathway. EMBO J. 20:2001;6735-6741.