Detection of Hepatitis C Virus RNA in Formalin-Fixed Paraffin-Embedded Sections with Digoxigenin-Labeled cRNA Probes

Diagnostic Molecular Pathology

Volumn 13, Issue 1, 2004, Pages 9-14

  Qian, Xiang ^a   Guerrero, Ruben B ^a   Plummer, Timothy B ^a   Alves, Venancio F ^b   Lloyd, Ricardo V ^a,c  

^a MAYO CLINIC   (United States)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c Dept of Lab Medicine and Pathology ^*  (United States)

Author keywords

Hepatitis C; In situ hybridization; Liver; Paraffin section; RT PCR

Indexed keywords

COMPLEMENTARY DNA; PARAFFIN; VIRUS RNA;

ARTICLE; CLINICAL ARTICLE; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; LIVER BIOPSY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; VIRUS DETECTION; VIRUS GENOME; VIRUS HEPATITIS;

BIOPSY; DIGOXIGENIN; FIXATIVES; FORMALDEHYDE; HEPACIVIRUS; HEPATITIS ANTIBODIES; HEPATITIS C; HUMANS; IMMUNOCHEMISTRY; IN SITU HYBRIDIZATION; LIVER; PARAFFIN EMBEDDING; PROLACTIN; RETROSPECTIVE STUDIES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA PROBES; RNA, VIRAL; VIRAL PROTEINS;

EID: 1342281167     PISSN: 10529551     EISSN: None     Source Type: Journal    
DOI: 10.1097/00019606-200403000-00002     Document Type: Article

References (31)

1. Majid AM, Gretch DR. Current and future hepatitis C virus diagnostic testing: Problems and advancements. Microbes Infect. 2002;4:1227-1236.
2. Rosenberg S. Recent advances in the molecular biology of hepatitis C virus. J Mol Biol. 2001;313:451-464.
3. Scheuer PJ, Krawczynski K, Dhillon AP. Histopathology and detection of hepatitis C virus in liver. Springer Semin Immunopathol. 1997;19:27-45.
4. Ohishi M, Sakisaka S, Harada M, et al. Detection of hepatitis-C virus and hepatitis-C virus replication in hepatocellular carcinoma by in situ hybridization. Scandinavian J Gastroenterol. 1999;34:432-438.
5. Negro F, Pacchioni D, Shimizu Y, et al. Detection of intrahepatic replication of hepatitis C virus RNA by in situ hybridization and comparison with histopathology. Proc Natl Acad Sci U S A. 1992;89:2247-2251.
6. Lerat H, Berby F, Trabaud M, et al. Specific detection of hepatitis C virus minus strand RNA in Hematopoietic Cells. Clin. Invest. 1996;97:845-851.
7. Negro F, Giostra E, Krawczynski K, et al. Detection of intrahepatic hepatitis C virus replication by strand-specific semi-quantitative RT-PCR: Preliminary application to the liver transplantation model. J Hepatol. 1998;29:1-11.
8. Nouri Aria KT, Sallie R, Sangar D, et al. Detection of genomic and intermediate replicative strands of hepatitis C virus in liver tissue by in situ hybridization. J Clin Invest. 1993;91:2226-2234.
9. Gastaldi M, Massacrier A, Planells R, et al. Detection by in situ hybridization of hepatitis C virus positive and negative RNA strands using digoxigenin-labeled cRNA probes in human liver cells. J Hepatol. 1995;23:509-518.
10. Muratori L. In situ reverse transcriptase-polymerase chain reaction: an innovative tool for hepatitis C virus RNA detection and localization, and for quantification of infected cells. Eur J Histochem. 1998;42:133-136.
11. Komminoth P, Adams V, Long AA, et al. Evaluation of methods for hepatitis C virus detection in archival liver biopsies. Comparison of histology, immunohistochemistry, in-situ hybridization, reverse transcriptase polymerase chain reaction (RT-PCR) and in-situ RT-PCR. Pathol. Res Pract. 1994;190:1017-1025.
12. Walker FM, Dazza MC, Dauge MC, et al. Detection and localization by in situ molecular biology techniques and immunohistochemistry of hepatitis C virus in livers of chronically infected patients. J Histochem Cytochem. 1998;46:653-660.
13. Lau JY, Krawczynski K, Negro F, et al. In situ detection of hepatitis C virus-a critical appraisal. J Hepatol. 1996;(2Suppl):2443-2451.
14. Negro F. Detection of hepatitis C virus RNA by in situ hybridization: a critical appraisal. International J Clin Lab Res. 1994;24:198-202.
15. Guerrero RB, Batts KP, Burgart LJ, et al. Early detection of hepatitis C allograft reinfection after orthotopic liver transplantation: A molecular and histologic study. Mod Pathol. 2000;13:229-237.
16. Guerrero RB, Batts KP, Brandhagen DJ, et al. Effects of formalin fixation and prolonged block storage on detection of hepatitis C virus RNA in liver tissue. Diagn Mol Pathol. 1997;6:277-281.
17. Yamada S, Koji T, Nozawa M, et al. Detection of hepatitis C virus (HCV) RNA in paraffin embedded tissue sections of human liver of non-A, non-B hepatitis patients by in situ hybridization. J Clin Lab Anal. 1992;6:40-46.
18. Felgar RE, Montone KT, Furth EE. A rapid method for the detection of hepatitis C virus RNA by in situ hybridization. Mod Pathol. 1996;9:696-702.
19. Moldvay J, Deny P, Pol S, et al. Detection of hepatitis C virus RNA in peripheral blood mononuclear cells of infected patients by in situ hybridization. Blood. 1994;83:269-273.
20. Haruna Y, Hayashi N, Hiramatsu N, et al. Detection of hepatitis C virus RNA in liver tissues by an in situ hybridization technique. J Hepatol. 1993;18:96-100.
21. Sansonno D, Comacchiulo V, Iacobelli AR, et al. Demonstration and distribution of HCV RNA sequences by in situ hybridization and HCV related proteins by immunohistochemistry in the liver tissue of patients with chronic HCV infection. Pathobiol. 1995;63:239-248.
22. Ohsawa M, Tomita Y, Hashimoto M, et al. Hepatitis C viral genome in a subset of primary hepatic lymphomas. Mod Pathol. 1998;11:471-478.
23. Ortiz-Movilla N, Lazaro P, Rodriguez-Inigo E, et al. Hepatitis C virus replicates in sweat glands and is released into sweat in patients with chronic hepatitis C. J Med Virol. 2002;68:529-536.
24. Tursi A, Brandimante G, Chiarelli F, et al. Detection of HCV RNA in gastric mucosa-associated lymphoid tissue by in situ hybridization: evidence of a new extrahepatic localization of HCV with increased risk of gastric malt lymphoma. Am J Gastroenterol. 2002;97:1802-1806.
25. Lazaro P, Olalquiaga J, Bartolome J, et al. Detection of hepatitis C virus RNA and core protein in keratinocytes from patients with cutaneous lichen planus and chronic hepatitis C. J Invest Dermatol. 2002;119:798-803.
26. Arrieta JJ, Rodriguez-Inigo E, Ortiz-Movilla N, et al. In situ detection of hepatitis C virus RNA in salivary glands. Am J Pathol. 2001;158:259-264.
27. Qian X, Bauer RA, Xu HS, et al. In situ hybridization detection of calcitonin mRNA in routinely fixed, paraffin-embedded tissue sections: a comparison of different types of probes combined with tyramide signal amplification. Appl Immunohisto Mol Morphol. 2001;9:61-69.
28. Hayden RT, Qian X, Roberts GD, et al. In situ hybridization for the identification of yeastlike organisms in tissue section. Diagn Mol Pathol. 2001;10:15-23.
29. Sternberger LA. The unlabeled antibody (PAP) method, introduction. J Histochem Cytochem. 1979;27:1657-1660.
30. Nuovo GJ, Lidonnici K, MacConnell P, et al. Intracellular localization of polymerase chain reaction (PCR)-amplified hepatitis C cDNA. Am J Surg Pathol. 1993;17:683-690.
31. Chang M, Marquardt AP, Wood BL, et al. In situ distribution of hepatitis C virus replicative-intermediate RNA in hepatic tissue and its correlation with liver disease. J Virol. 2000;74:944-955.