Mutational studies using a cation-conducting GABAA receptor reveal the selectivity determinants of the Cys-loop family of ligand-gated ion channels

Journal of Neurochemistry

Volumn 92, Issue 4, 2005, Pages 962-972

  Jensen, Marianne L ^a,b   Pedersen, Lone N ^a   Timmermann, Daniel B ^a   Schousboe, Arne ^b   Ahring, Philip K ^a  

^a NEUROSEARCH A S   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Cys loop receptor; GABAA receptor; Ion selectivity

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; CATION CHANNEL; NICOTINIC RECEPTOR; SEROTONIN RECEPTOR;

ARTICLE; CELL MEMBRANE PERMEABILITY; CHANNEL GATING; CHINESE HAMSTER; HIPPOCAMPUS; HUMAN; HUMAN CELL; HUMAN TISSUE; ION TRANSPORT; MATHEMATICAL ANALYSIS; MATHEMATICAL COMPUTING; MUTATIONAL ANALYSIS; NONHUMAN; OVARY CELL; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; ANIMALS; CATION TRANSPORT PROTEINS; CHO CELLS; CRICETINAE; CYSTEINE; DNA MUTATIONAL ANALYSIS; HUMANS; ION CHANNEL GATING; ION CHANNELS; LIGANDS; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, GABA-A;

EID: 13244271097     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1471-4159.2004.02970.x     Document Type: Article

References (24)

1. Akabas M. H., Kaufmann C., Archdeacon P. and Karlin A. (1994) Identification of acetylcholine receptor channel-lining residues in the entire M2 segment of the alpha subunit. Neuron 13, 919-927.
2. Corringer P. J., Bertrand S., Galzi J. L., Devillers-Thiery A., Changeux J. P. and Bertrand D. (1999) Mutational analysis of the charge selectivity filter of the alpha7 nicotinic acetylcholine receptor. Neuron 22, 831-843.
3. 2+. J. Biol. Chem. 278, 712-717.
4. Fatima-Shad K. and Barry P. H. (1993) Anion permeation in. Proc. R. Soc. Lond. B Biol. Sci. 253, 69-75.
5. Galzi J. L., Devillers-Thiery A., Hussy N., Bertrand S., Changeux J. P. and Bertrand D. (1992) Mutations in the channel domain of a neuronal nicotinic receptor convert ion selectivity from cationic to anionic. Nature 359, 500-505.
6. Goldman D. E. (1943) Potential, impedance, and rectification in membranes. J. Gen. Physiol. 27, 37-60.
7. Gunthorpe M. J. and Lummis S. C. (2001) Conversion of the ion selectivity of the 5-HT(3a) receptor from cationic to anionic reveals a conserved feature of the ligand-gated ion channel superfamily. J. Biol. Chem. 276, 10 977-10 983.
8. Hamill O. P., Marty A., Neher E., Sakmann B. and Sigworth F. J. (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 391, 85-100.
9. Hodgkin A. L. and Katz B. (1949) The effect of sodium ions on the electrical activity of the giant axon of the squid. J. Physiol. 108, 37-77.
10. A receptor. J. Biol. Chem. 277, 41 438-41 447.
11. Karlin A. and Akabas M. H. (1995) Toward a structural basis for the function of nicotinic acetylcholine receptors and their cousins. Neuron 15, 1231-1244.
12. Keramidas A., Moorhouse A. J., French C. R., Schofield P. R. and Barry P. H. (2000) M2 pore mutations convert the glycine receptor channel from being anion- to cation-selective. Biophys. J. 79, 247-259.
13. Keramidas A., Moorhouse A. J., Pierce K. D., Schofield P. R. and Barry P. H. (2002) Cation-selective mutations in the M2 domain of the inhibitory glycine receptor channel reveal determinants of ion-charge selectivity. J. Gen. Physiol. 119, 393-410.
14. Langosch D., Thomas L. and Betz H. (1988) Conserved quaternary structure of ligand-gated ion channels: the postsynaptic glycine receptor is a pentamer. Proc. Natl Acad. Sci. USA 85, 7394-7398.
15. Lee D. J., Keramidas A., Moorhouse A. J., Schofield P. R. and Barry P. H. (2003) The contribution of proline 250 (P-2′) to pore diameter and ion selectivity in the human glycine receptor channel. Neurosci. Lett. 351, 196-200.
16. Leonard R. J., Labarca C. G., Charnet P., Davidson N. and Lester H. A. (1988) Evidence that the M2 membrane-spanning region lines the ion channel pore of the nicotinic receptor. Science 242, 1578-1581.
17. Maricq A. V., Peterson A. S., Brake A. J., Myers R. M. and Julius D. (1991) Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel. Science 254, 432-431.
18. Miller C. (1989) Genetic manipulation of ion channels: a new approach to structure and mechanism. Neuron 2, 1195-1205.
19. A receptor determined by electron microscopic image analysis. J. Neurochem. 62, 815-818.
20. Raftery M. A., Hunkapiller M. W., Strader C. D. and Hood L. E. (1980) Acetylcholine receptor: complex of homologous subunits. Science 208, 1454-1456.
21. Reeves D. C., Goren E. N., Akabas M. H. and Lummis S. C. (2001) Structural and electrostatic properties of the 5-HT3 receptor pore revealed by substituted cysteine accessibility mutagenesis. J. Biol. Chem. 276, 42 035-42 042.
22. Thompson A. J. and Lummis S. C. (2003) A single ring of charged amino acids at one end of the pore can control ion selectivity in the 5-HT3 receptor. Br. J. Pharmacol. 140, 359-365.
23. Xu M. and Akabas M. H. (1993) Amino acids lining the channel of the gamma-aminobutyric acid type A receptor identified by cysteine substitution. J. Biol. Chem. 268, 21 505-21 508.
24. Xu M. and Akabas M. H. (1996) Identification of channel-lining residues in the M2 membrane-spanning segment of the GABA(A) receptor alphal subunit. J. Gen. Physiol. 107, 195-205.