Metabolism of polychlorinated dibenzo-p-dioxins by cytochrome P450 BM-3 and its mutant

Biotechnology Letters

Volumn 26, Issue 24, 2004, Pages 1857-1860

  Sulistyaningdyah, Woro Triarsi ^a   Ogawa, Jun ^a   Li, Qing Shan ^a   Shinkyo, Raku ^a   Sakaki, Toshiyuki ^a,c   Inouye, Kuniyo ^a   Schmid, Rolf D ^b   Shimizu, Sakayu ^a  

^a KYOTO UNIVERSITY   (Japan)

^b UNIVERSITY OF STUTTGART   (Germany)

^c TOYAMA PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Bioremediation; Cytochrome P450; Dioxin; P450 BM 3; Polychlorinated dibenzo p dioxin

Indexed keywords

DERIVATIVES; ENZYMES; HYDROXYLATION; METABOLISM; MICROORGANISMS; TOXICITY;

CYTOCHROMES; MUTANT ENZYMES; POLYCHLORINATED DIBENZO-P-DIOXINS; TOXIC DERIVATIVES;

AROMATIC HYDROCARBONS;

2,3,7,8 TETRACHLORODIBENZO PARA DIOXIN; CYTOCHROME P450; DRUG DERIVATIVE; POLYCHLORODIBENZO 4 DIOXIN; POLYCHLORODIBENZO-4-DIOXIN;

ARTICLE; BACILLUS MEGATERIUM; BIOREMEDIATION; COMPARATIVE STUDY; EVALUATION; GENETICS; METABOLISM; METHODOLOGY; MUTATION; PROTEIN ENGINEERING; SOIL POLLUTANT; SPECIES DIFFERENCE;

BACILLUS MEGATERIUM; BIODEGRADATION, ENVIRONMENTAL; CYTOCHROME P-450 ENZYME SYSTEM; MUTATION; PROTEIN ENGINEERING; SOIL POLLUTANTS; SPECIES SPECIFICITY; TETRACHLORODIBENZODIOXIN;

BACILLUS MEGATERIUM;

EID: 13244265817     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10529-004-5317-y     Document Type: Article

References (13)

1. Armengaud J, Happe B, Timmis K (1998) Genetic analysis of dioxin dioxygenase of Sphingomonas sp. strain RW1: catabolic genes dispersed on the genome. J. Bacteriol. 180: 3954-3966.
2. Beurskens JEM, Toussaint M, De Wolf J, Van der Steen JMD, Slot PC, Commandeur LCM, Parsons JR (1995) Dehalogenation of chlorinated dioxins by an anaerobic microbial consortium from sediment. Environ. Toxicol. Chem. 14: 939-943.
3. Bumpus JA, Tien M, Wright D, Aust SD (1985) Oxidation of persistent environmental pollutants by a white rot fungus. Science 228: 1434-1436.
4. Inouye K, Shinkyo R, Takita T, Ohta M, Sakaki T (2002) Metabolism of polychlorinated dibenzo-p-dioxins (PCDDs) by human cytochrome P450-dependent monooxygenase system. J. Agric. Food Chem. 50: 5496-5502.
5. Li QS, Ogawa J, Schmid RD, Shimizu S (2001a) Engineering cytochrome P450 BM-3 for oxidation of polycyclic aromatic hydrocarbons. App. Environ. Microbiol. 67: 5735-5739.
6. Li QS, Ogawa J, Schmid RD, Shimizu S (2001b) Residue size at position 87 of cytochrome P450 BM-3 determines its stereoselectivity in propylbenzene and 3-chlorostyrene oxidation. FEBS Lett. 508: 249-252.
7. Li QS, Schwaneberg U, Fischer M, Schmitt J, Pleiss J, Lutz-Wahl S, Schmid RD (2001c) Rational evolution of a medium chain-specific cytochrome P-450 BM-3 variant. Biochim. Biophys. Acta 1545: 114-121.
8. Li QS, Schwaneberg U, Fischer P, Schmid RD (2000) Directed evolution of the fatty-acid hydroxylase P450 BM-3 into an indol-hydroxylating catalyst. Chem. Eur. J. 6: 1531-1536.
9. Nojiri H, Nam JW, Kosaka M, Morii K, Takemura T, Furihata K, Yamane H, Omori T (1999) Diverse oxygenations catalyzed by carbazole 1,9a-dioxygenase from Pseudomonas sp. strain CA10. J. Bacteriol. 181: 3105-3113.
10. Sakaki T, Shinkyo R, Takita T, Ohta M, Inouye K (2002) Biodegradation of polychlorinated dibenzo-p-dioxins by recombinant yeast expressing rat CYP1A subfamily. Arch. Biochem. Biophys. 401: 91-98.
11. Sato S, Nam JW, Kasuga K, Nojiri H, Yamane H, Omori T (1997) Identification and characterization of genes encoding carbazole 1,9a-dioxygenase in Pseudomonas sp. strain CA10. J. Bacteriol. 179: 4850-4858.
12. Shinkyo R, Sakaki T, Ohta M, Inouye K (2003) Metabolic pathways of dioxin by CYP1A1: species difference between rat and human CYP1A subfamily in the metabolism of dioxins. Arch. Biochem. Biophys. 409: 180-187.
13. Van Leeuwen FXR, Feeley M, Schrenk D, Larsen JC, Farland W, Younes M (2000) Dioxins: WHO's tolerable daily intake (TDI) revisited. Chemosphere 40: 1095-1101.