Gram-staining characterisation of activated sludge filamentous bacteria by automated colour analysis

Biotechnology Letters

Volumn 26, Issue 24, 2004, Pages 1841-1846

  Pandolfi, Denis ^a   Pons, Marie Noëlle ^a  

^a UNIVERSITÉ DE LORRAINE   (France)

Author keywords

Activated sludge; Colour image analysis; Gram staining; Population shift

Indexed keywords

BACTERIA; CORRELATION METHODS; IMAGE ANALYSIS; ROBUSTNESS (CONTROL SYSTEMS);

ACTIVATED SLUDGE; BINARY METHODS; FILAMENTOUS BACTERIA; GRAM-STAINING;

SEWAGE SLUDGE;

CRYSTAL VIOLET; GRAM'S STAIN; PHENAZINE DERIVATIVE;

ARTICLE; AUTOMATED PATTERN RECOGNITION; BACTERIAL COUNT; COLORIMETRY; COMPARATIVE STUDY; COMPUTER ASSISTED DIAGNOSIS; CYTOLOGY; EVALUATION; FUZZY LOGIC; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; PILOT STUDY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SEWAGE; VALIDATION STUDY;

COLONY COUNT, MICROBIAL; COLORIMETRY; FUZZY LOGIC; GENTIAN VIOLET; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; IMAGE INTERPRETATION, COMPUTER-ASSISTED; PATTERN RECOGNITION, AUTOMATED; PHENAZINES; PILOT PROJECTS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEWAGE;

BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 13244261029     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10529-004-5280-7     Document Type: Article

References (12)

1. Clark G (1981) Staining Procedures. 4th edn. Baltimore, MD: Williams & Wilkins.
2. da Motta M, Pons MN, Roche N (2001) Automated monitoring of activated sludge in pilot plant using image analysis. Water Sci. Technol. 43: 91-96.
3. Jenkins D, Richard MG, Daigger GT (1993) Manual on the Causes and Control of Activated Sludge Bulking and Foaming. Boca Raton, FL: Lewis Publishers.
4. Manz W, Amann R, Ludwig W, Wagner M, Schleifer KH (1992) Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: problems and solutions. System. Appl. Microbiol. 15: 593-600.
5. Pernelle JJ, Cotteux E, Duchène P (1998) Effectiveness of oligonucleotide probes targeted against Thiothrix nivea and type 021N 16S RNA for in situ identification and population monitoring in activated sludges. Water Sci. Technol. 37: 431-440.
6. Pernelle JJ, Gaval G, Cotteux E, Duchène P (2001) Influence of transient substrate overloads on the proliferation of filamentous bacterial populations in an activated sludge pilot plant. Water Res. 35: 129-134.
7. Roller C, Wagner M, Amann R, Ludwig W, Schleifer KH (1994) In situ probing of Gram-positive bacteria with high DNA G+C content using 23S rRNA-targeted oligonucleotides. Microbiology 140: 2849-2858.
8. Russ JC (1995) The Image Processing Handbook, 2nd edn., Boca Raton, FL: CRC Press.
9. Saida H, Maeekawa T, Satake T, Higashi Y, Seki H (2000) Gram stain index of natural bacteria community at a nutrient gradient in freshwater environment. Environ. Pollut. 109: 293-301.
10. Saida H, Ytow N, Seki H (1998) Photometric application of Gram stain method to characterize natural bacterial populations in aquatic environments. Appl. Environ. Microbiol. 64: 742-747.
11. Salton MRJ (1964) The Bacterial Cell Wall. Amsterdam, The Netherlands: Elsevier.
12. Wagner M, Ammann R, Kämpfer P, Assmus B, Hartmann A, Hutzler P, Springer N, Schleifer KH (1994) Identification and in situ detection of Gram-negative filamentous bacteria in activated sludge. System. Appl. Microbiol. 17: 405-417.