Photometric application of the gram stain method to characterize natural bacterial populations in aquatic environments

Applied and Environmental Microbiology

Volumn 64, Issue 2, 1998, Pages 742-747

  Saida, H ^a   Ytow, N ^a   Seki, H ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AQUATIC FLORA; ARTICLE; BACILLUS SUBTILIS; BACTERIUM COLONY; CAMERA; CONTROLLED STUDY; ESCHERICHIA COLI; EUTROPHICATION; GRAM STAINING; IMAGE ANALYSIS; MEMBRANE FILTER; NONHUMAN; PHOTOMETRY;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA;

EID: 0031890705     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.2.742-747.1998     Document Type: Article

References (23)

1. Beveridge, T. J. 1981. Ultrastructure, chemistry and function of the bacterial wall. Int. Rev. Cytol. 72:229-317.
2. Beveridge, T. J. 1990. Mechanism of Gram variability in select bacteria. J. Bacteriol. 172:1609-1620.
3. Beveridge, T. J., and J. A. Davies. 1983. Cellular responses of Bacillus subtilis and Escherichia coli to the Gram stain. J. Bacteriol. 156:846-858.
4. Bowden, W. B. 1977. Comparison of two direct-count techniques for enumerating aquatic bacteria. Appl. Environ. Microbiol. 33:1229-1232.
5. Brock, T. D., M. T. Madigan, J. M. Martinko, and J. Parker. 1994. Biology of microorganisms, 7th ed. Prentice-Hall International, Inc., Englewood Cliffs, N.J.
6. Davies, J. A., G. K. Anderson, T. J. Beveridge, and H. C. Clark. 1983. Chemical mechanism of the Gram stain and synthesis of a new electron-opaque marker for electron microscopy which replaces the iodine mordant of the stain. J. Bacteriol. 156:837-845.
7. Greenberg, A. E., L. S. Clesceri, and A. D. Eaton. 1992. Standard methods for the examination of water and wastewater, 18th ed. American Public Health Association, Washington, D.C.
8. Hara, Y., A. Tsuchida, and H. Seki. 1983. Spring bloom in a hypereutrophic lake, Lake Kasumigaura, Japan. II. Succession of phytoplankton species. Water Res. 17:447-451.
9. Harold, F. M. 1972. Conservation and transformation of energy by bacterial membranes. Bacteriol. Rev. 36:172-230.
10. Higashi, Y., N. Ytow, and H. Seki. 1997. In situ gradostat for the study of natural phytoplankton community with experimental nutrient gradostat. In Abstracts of the Spring 1997 General Meeting Oceanographic Society of Japan. (In Japanese.)
11. Hobbie, J. E., R. J. Daley, and S. Jasper. 1977. Use of Nuclepore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33:1225-1228.
12. Murray, R. G. E., R. N. Doetsch, and C. F. Robinow. 1994. Determinative and cytological light microscopy, p. 22-41. In P. Gerhardt, R. G. E. Murray, W. A. Wood, and N. R. Krieg (ed.), Methods for general and molecular bacteriology. American Society for Microbiology, Washington, D.C.
13. Naganuma, T., and H. Seki. 1985. Population growth rate of the bacterio-plankton population in a bog, Matsumi-ike, Japan. Arch. Hydrobiol. 104: 543-556.
14. Nakamura, K., E. Hashizume, T. Shibata, Y. Nakamura, S. Mala, and K. Yamane. 1995. Small cytoplasmic RNA (scRNA) gene from Clostridium perfringens can replace the gene for the Bacillus subtilis scRNA in both growth and sporulation. Microbiology 141:2965-2975.
15. Parsons, T. R., Y. Maita, and C. Lalli. 1984. A manual of chemical and biological methods for seawater analysis. Pergamon Press, Oxford, United Kingdom.
16. Rodina, A. G. 1972. Methods in aquatic microbiology. University Park Press, Baltimore, Md.
17. Sasa, M., S. Ishizaka, T. Asamizu, M. Aoki, Y. Nojiri, Y. Higashi, M. Utsumi, H. Zeng, N. Ytow, and H. Seki. 1996. Ecosystem structure of a boiling spring with high bacterial production on Mt. Tateyama, Japan. Arch. Hydrobiol. 136:563-574.
18. Seki, H., and E. Takahashi. 1983. Spring bloom in a hypereutrophic lake, Lake Kasumigaura, Japan. I. Succession of phytoplankters with different accessory pigments. Water Res. 17:441-445.
19. Seki, H., and A. Masaki. 1983. Spring bloom in a hypereutrophic lake, Lake Kasumigaura, Japan. III. Phytoplankton abundance in standing stock of organic matter in lake water. Water Res. 17:453-457.
20. Seki, H., A. Otsuki, S. Daigobo, C. D. Levings, and C. D. Mcallister. 1984. Microbial contribution to the mesotrophic ecosystem of the Campbell River Estuary during summer. Arch. Hydrobiol. 102:215-228.
21. Seki, H., and T. Naganuma. 1989. Growth characteristics of Thiobacterium sp. from the plume of hydrothermal vents of the North Fiji Basin. Mar. Ecol. Prog. Ser. 54:199-202.
22. Shiraishi, K., Y. Uno, Y. Hara, and H. Seki. 1985. Factors controlling phytoplankton population in a bog, Matsumi-ike, Japan. Arch. Hydrobiol. 104: 387-406.
23. Tsuchida, A., Y. Hara, and H. Seki. 1984. Spring bloom in a hypereutrophic lake, Lake Kasumigaura, Japan. V. Factors controlling natural population of phytoplankters. Water Res. 18:877-883.