Amino-terminal dimerization of an erythropoietin mimetic peptide results in increased erythropoietic activity

Chemistry and Biology

Volumn 4, Issue 12, 1997, Pages 939-950

  Johnson, Dana L ^a   Farrell, Francis X ^a   Barbone, Francis P ^a   McMahon, Frank J ^a   Tullai, Jennifer ^a   Kroon, Daniel ^b   Freedy, James ^b   Zivin, Robert A ^a   Mulcahy, Linda S ^a   Jolliffe, Linda K ^a  

^a Drug Discovery Raritan   (United States)

^b R W JOHNSON PHARMACEUTICAL RES I   (United States)

Author keywords

Dimerization; Erythropoietin mimetics; Erythropoietin receptor; Peptides; Polyethylene glycol

Indexed keywords

EID: 13144252172     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(97)90302-1     Document Type: Article

References (27)

1. Krantz, S.B. (1991). Erythropoietin. Blood 77, 419-434.
2. Fried, W. (1995). Erythropoietin. Annu. Rev. Nutr. 15, 353-377.
3. Jones, S.S., D'Andrea, A.D., Haines, L.L, & Wong, G.G. (1990). Human erythropoietin receptor: Cloning, expression and biologic characterization. Blood 76, 31-35.
4. Noguchi, C.T., Bae, K.S., Chin, K., Wada, Y., Schecter, A.N. & Hankins, W.D. (1991). Cloning of the human erythropoietin receptor gene. Blood 78, 2548-2556.
5. Maouche, L., Tournamile C., Hattab, C., Boffa, G., Carton J.-P. & Chretein, S. (1991). Cloning of the gene encoding the human erythropoietin receptor. Blood 78, 2557-2563.
6. Wrighton, N.C., et al., & Dower, W.J. (1996) Small peptides as potent mimetics of the protein hormone erythropoietin. Science 273, 458-463.
7. Livnah, O., et al., & Wilson, I.A. (1996). Functional mimicry of a protein hormone by a peptide agonist: the EPO receptor complex at 2.8 Å. Science 273, 464-471.
8. Elliot, S., Lorenzini, T., Yanagihara, D. Chang, D. & Elliot, G. (1996). Activation of the erythropoietin (EPO) receptor by bivalent anti-EPO receptor antibodies. J. Biol. Chem. 271, 24691-24697.
9. Schneider, H., et al., & Giebel, LB. (1997). Homodimerization of erythropoietin receptor by a bivalent monoclonal antibody triggers cell proliferation and differentiation of erythroid precursors. Blood 89, 473-482.
10. Yoshimura, A., Longmore, G., & Lodish, H.F. (1994). Point mutation in the exoplasmic domain of the erythropoietin receptor resulting in hormone-independent activation and tumorigenicity. Nature 348, 647-649.
11. Watowich, S.S., Yoshimura, A., Longmore, G.D., Hilton, D.S., Yohimura, Y. & Lodish, H.F. (1992). Homodimerization and constitutive activation of the erythropoietin receptor. Proc. Natl Acad. Sci. USA 89, 2140-2145.
12. Watowich, S.S., Hilton, D.S. & Lodish, H.F. (1994). Activation and inhibition of erythropoietin receptor function: role of receptor dimerization. Mol. Cell. Biol. 14, 3535-3549.
13. Philo, J. S., Aoki, K. H., Arakawa, T., Owens-Narhi, L., & Wan, J. (1996). Dimerization of the extracellular domain of the erythropoietin (EPO) receptor by EPO: one high-affinity and one low affinity interaction. Biochemistry 35, 1681-1691.
14. Wells, J.A. & de Vos A.M. (1996). Hematopoietic receptor complexes. Annu. Rev. Biochem. 65, 609-634.
15. Delgado, C., Francis, G.E. & Fisher, D. (1992). The uses and properties of PEG-linked Proteins. Crit. Rev. Ther. Drug Carrier Syst. 9, 249-304.
16. Johnson, D.L., et al., & Jolliffe L.K., (1997). Identification of a 13 amino acid peptide mimetic of erythropoietin and description of amino acids critical for the mimetic activity of EMP1. Biochemistry, in press.
17. Wrighton, N.C., et al., & Dower, W.J. (1996). Small peptide mimetics of erythropoietin. Blood 88 (Suppl 1), 543a.
18. Seed, B. (1994). Making agonists of antagonists. Chem Biol 1, 125-129.
19. Austin, D.J., Crabtree, G.R. & Schreiber, S.L. (1994). Proximity versus allostery: the role of regulated protein dimerization in biology. Chem Biol 1, 131-136.
20. Cwirla, S.E., et al., & Dower, W.J. (1997) Peptide agonist of thrombopoietin as potent as the natural cytokine. Science 276, 1696-1699.
21. Dai, W., Belt, J. &. Saltzman, W.M. (1994). Cell binding peptides conjugated to poly(ethylene) glycol promote neural cell aggregation. Biotechnology (NY) 12, 797-801.
22. Johnson, D.L., et al., & Jolliffe, L.K. (1996). Refolding, purification and characterization of human erythropoietin binding protein produced in E.coli. Protein Expr. Purif. 7, 104-113.
23. Middleton, S.A., et al., & Mulcahy, L.S., (1996). Identification of a critical ligand binding determinant of the human erythropoietin receptor. J. Biol. Chem. 271, 14045-14054.
24. Barbone, F.P., et al, & Mulcahy, L.S., (1997). Mutagenesis studies of the human erythropoietin receptor: establishment of structure-function relationships. J. Biol. Chem. 272, 4985-4992.
25. Kitamura, T., Tojo, A., Kuwaki, T., Chiba, S., Miyazono, K., Urabe, A. & Takaku, F. (1989) Identification and analysis of human erythropoietin receptors on a factor dependent cell line, TF-1. Blood 73, 375-380.
26. Caroll, M.P., et al., & May, W.S. (1991). Erythropoietin induces Raf-1 activation and Raf-1 is required for erythropoietin induced proliferation. J. Biol. Chem. 266, 14964-14969.
27. Cotes, P.M. & Bangham, D.R. (1961). Bio-assay of erythropoietin in mice made polycythemic by exposure to air at reduced pressure. Nature 191, 1065-1067.