Ex vivo cultured megakaryocytes express functional glycoprotein IIb-IIIa receptors and are capable of adenovirus-mediated transgene expression

Blood

Volumn 94, Issue 12, 1999, Pages 4084-4092

  Faraday, Nauder ^a   Rade, Jeffrey J ^b   Johns, David C ^b   Khetawat, Gopal ^b   Noga, Stephen J ^b   DiPersio, John F ^b   Jin, Ying ^b   Nichol, Janet L ^b   Haug, Jeff S ^b   Bray, Paul F ^b  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; FIBRINOGEN; FIBRINOGEN RECEPTOR; GREEN FLUORESCENT PROTEIN; VIRUS VECTOR;

ARTICLE; CELL DIFFERENTIATION; CONTROLLED STUDY; FLOW CYTOMETRY; GENE EXPRESSION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; MEGAKARYOCYTE; PRIORITY JOURNAL; RECEPTOR UPREGULATION; THROMBOCYTE FUNCTION; TRANSGENE;

EID: 13044312102     PISSN: 00064971     EISSN: None     Source Type: Journal    
DOI: 10.1182/blood.v94.12.4084     Document Type: Article

References (37)

1. Gewirtz AM, Schick B: Megakaryocytopoiesis, in Colman RW, Hirsh J, Marder VJ, Salzman WE (eds): Hemostasis and Thrombosis: Basic Principles and Clinical Practice. Philadelphia, PA, Lippincott, 1994, p 353
2. Bennett JS, Vilaire G: Exposure of platelet fibrinogen receptors by ADP and epinephrine. J Clin Invest 64:1393, 1979
3. Bodary SC, Napier MA, McLean JW: Expression of recombinant platelet glycoprotein IIbIIIa results in a functional fibrinogen-binding complex. J Biol Chem 264:18859, 1989
4. Shattil SJ, Cunningham M, Hoxie JA: Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood 70:307, 1987
5. Greenberg SM, Rosenthal DS, Greeley TA, Tantravahi R, Handin RI: Characterization of a new megakaryocytic cell line: The Dami cell. Blood 72:1968, 1988
6. Ogura M, Morishima Y, Okumura M, Hotta T, Takamoto S, Ohno R, Hirabayashi N, Nagura H, Saito H: Functional and morphological differentiation induction of a human megakaryoblastic leukemia cell line (MEG-01s) by phorbol diesters. Blood 72:49, 1988
7. Mazur EM, Hoffman R, Bruno E: Regulation of human megakaryocytopoiesis. An in vitro analysis. J Clin Invest 68:733, 1981
8. Mazur EM, Basilico D, Newton JL, Cohen JL, Charland C, Sohl PA, Narendran A: Isolation of large numbers of enriched human megakaryocytes from liquid cultures of normal peripheral blood progenitor cells. Blood 76:1771, 1990
9. Guerriero R, Testa U, Gabbianelli M, Mania G, Montesoro E, Macioce G, Pace A, Ziegler B, Hassan HJ, Peschle C: Unilineage megakaryocytic proliferation and differentiation of purified hematopoietic progenitors in serum-free liquid culture. Blood 86:3725, 1995
10. Bartley TD, Bogenberger J, Hunt P, Li YS, Lu HS, Martin F, Chang MS, Samal B, Nichol JL, Swift S, Johnson J, Hsu R-Y, Parker VP, Suggs S, Skrine JD, Merewether LA, Clogston C, Hsu E, Hokom MM, Hornkohl A, Choi E, Pangelinan M, Sun Y, Mar V, McNinch J, Simonet L, Jacobsen F, Xie C, Shutter J, Chute H, Basu R, Selander L, Trollinger D, Sieu L, Padilla D, Trail G, Elliott G, Izumi R, Covey T, Crouse J, Garcia A, Xu W, Del Castillo J, Biron J, Cole S, Hu MC-T, Pacifici R, Ponting I, Saris C, Wen D, Yung YP, Lin H, Bosselman RA: Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor Mpl. Cell 77:1117, 1994
11. de Sauvage FJ, Hass PE, Spencer SD, Malloy BE, Gurney AL, Spencer SA, Darbonne WC, Henzel WJ, Wong SC, Kuang WJ, Oles KJ, Hultgren B, Solberg LA Jr, Goeddel DV, Eaton DL: Stimulation of megakaryocytopoiesis and thrombopoiesis by the c-Mpl ligand. Nature 369:533, 1994
12. Lok S, Kaushansky K, Holly RD, Kuijper JL, Lofton-Day CE, Oort PJ, Grant FJ, Heipel MD, Burkhead SK, Kramer JM, Bell LA, Sprecher CA, Blumberg H, Johnson R, Prunkard D, Ching AFT, Mathewes SL, Bailey MC, Forstrom JW, Buddle MM, Osbora SG, Evans SJ, Sheppard PO, Presnell SR, O'Hara PJ, Hagen FS, Roth GJ, Foster DC: Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo. Nature 369:565, 1994
13. Kaushansky K, Lok S, Holly RD, Broudy VC, Lin N, Bailey MC, Forstrom JW, Buddle MM, Oort PJ, Hagen FS, Roth GJ, Papanyannopoulou T, Foster DC: Promotion of megakaryocyte progenitor expansion and differentiation by the c-Mpl ligand thrombopoietin. Nature 369:568, 1994
14. Nichol JL, Hokom MM, Hornkohl A, Sheridan WP, Ohashi H, Kato T, Li YS, Bartley TD, Choi E, Bogenberger J, Skrine JD, Knudten A, Chen J, Trail G, Sleeman L, Cole S, Grampp G, Hunt P: Megakaryocyte growth and development factor. Analyses of in vitro effects on human megakaryopoiesis and endogenous serum levels during chemotherapy-induced thrombocytopenia. J Clin Invest 95: 2973, 1995
15. Faraday N, Goldschmidt-Clermont P, Dise K, Bray PF: Quantitation of soluble fibrinogen binding to platelets by fluorescence-activated flow cytometry. J Lab Clin Med 123:728, 1994
16. Qiao X, Loudovaris M, Unverzagt K, Walker DE, Smith SL, Martinson J, Schilling M, Lee W, Williams SF, Van Epps DE, Cohen I, Bender JG: Immunocytochemistry and flow cytometry evaluation of human megakaryocytes in fresh samples and cultures of CD34+ cells. Cytometry 23:250, 1996
17. Fattorossi A, Nisini R, Pizzolo JG, D'Amelio R: New, simple flow cytometric technique to discriminate between internalized and membrane-bound particles in phagocytosis. Cytometry 10:320, 1989
18. Nichols JE, Mock DJ, Roberts NJ: Use of FITC-labeled influenza virus and flow cytometry to assess binding and internalization of virus by monocytes-macrophages and lymphocytes. Arch Virol 130: 441, 1992
19. Johns DC, Nuss HB, Chiamvimonvat N, Ramza BM, Marban E, Lawrence JH: Adenovirus-mediated expression of a voltage-gated potassium channel in vitro (rat cardiac myocytes) and in vivo (rat liver). A novel strategy for modifying excitability. J Clin Invest 96:1152, 1995
20. Johns DC, Nuss HB, Marban E: Suppression of neuronal and cardiac transient outward currents by viral gene transfer of dominant-negative Kv4.2 constructs. J Biol Chem 272:31598, 1997
21. Hardy S, Kitamura M, Harris-Stansil T, Dai Y, Phipps ML: Construction of adenovirus vectors through Cre-lox recombination. J Virol 71:1842, 1997
22. Lee SW, Trapnell BC, Rade JJ, Virmani R, Dichek DA: In vivo adenoviral vector-mediated gene transfer into balloon-injured rat carotid arteries. Circ Res 73:797, 1993
23. Tcheng JE, Harrington RA, Kottke-Marchant K, Kleiman NS, Ellis SG, Kereiakes DJ, Mick MJ, Navetta FI, Smith JE, Worley SJ, Miller JA, Joseph DM, Sigmon KN, Kin MM, du Mée CP, Califf RM, Topol EJ, for the IMPACT Investigators: Multicenter, randomized, double-blind, placebo-controlled trial of the platelet integrin glycoprotein IIb/IIIa blocker Integrelin in elective coronary intervention. Circulation 91:2151, 1995
24. Plow EF, Pierschbacher MD, Ruoslahti E, Marguerie GA, Ginsberg MH: The effect of Arg-Gly-Asp-containing peptides on fibrinogen and von Willebrand factor binding to platelets. Proc Natl Acad Sci USA 82:8057, 1985
25. Coller BS, Peerschke EI, Scudder LE, Sullivan CA: A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and/or IIIa. J Clin Invest 72:325, 1983
26. Cheresh DA, Spiro RC: Biosynthetic and functional properties of an Arg-Gly-Asp-directed receptor involved in human melanoma cell attachment to vitronectin, fibrinogen, and von Willebrand factor. J Biol Chem 262:17703, 1987
27. Peerschke EI, Wainer JA: Examination of irreversible platelet-fibrinogen interactions. Am J Physiol 248:C466, 1985
28. Wencel-Drake JD, Boudignon-Proudhon C, Dieter MG, Criss AB, Parise LV: Internalization of bound fibrinogen modulates platelet aggregation. Blood 87:602, 1996
29. Handagama P, Scarborough RM, Shuman MA, Bainton DF: Endocytosis of fibrinogen into megakaryocyte and platelet alpha-granules is mediated by alpha IIb beta 3 (glycoprotein IIb-IIIa). Blood 82:135, 1993
30. Schick PK, Wojenski CM, He X, Walker J, Marcinkiewicz C, Niewiarowski S: Integrins involved in the adhesion of megakaryocytes to fibronectin and fibrinogen. Blood 92:2650, 1998
31. Vassallo R Jr, Kieber-Emmons T, Cichowski K, Brass LF: Structure-function relationships in the activation of platelet thrombin receptors by receptor-derived peptides. J Biol Chem 267:6081, 1992
32. Marguerie GA, Plow EF, Edgington TS: Human platelets possess an inducible and saturable receptor specific for fibrinogen. J Biol Chem 254:5357, 1979
33. Wencel-Drake JD, Frelinger AL, Dieter MG, Lam SC: Arg-Gly-Asp-dependent occupancy of GPIIb-IIIa by applaggin: Evidence for internalization and cycling of a platelet integrin. Blood 81:62, 1993
34. Huang MR, Olsson M, Kallin A, Pettersson U, Totterman TH: Efficient adenovirus-mediated gene transduction of normal and leukemic hematopoietic cells. Gene Ther 4:1093, 1997
35. Limon A, Briones J, Puig T, Carmona M, Fornas O, Cancelas JA, Nadal M, Garcia J, Rueda F, Barquinero J: High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells. Blood 90:3316, 1997
36. Bett AJ, Haddara W, Prevec L, Graham FL: An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3. Proc Natl Acad Sci USA 91:8802, 1994
37. Frey BM, Hackett NR, Bergelson JM, Finberg R, Crystal RG, Moore MAS, Rafii S: High-efficiency gene transfer into ex vivo expanded human hematopoietic progenitors and precursor cells by adenovirus vectors. Blood 91:2781, 1998