Processive action of cellobiohydrolase Cel7A from Trichoderma reesei is revealed as 'burst' kinetics on fluorescent polymeric model substrates

Biochemical Journal

Volumn 385, Issue 2, 2005, Pages 527-535

  Kipper, Kalle ^a   Väljamäe, Priit ^a   Johansson, Gunnar ^b  

^a UNIVERSITY OF TARTU   (Estonia)

^b UPPSALA UNIVERSITY   (Sweden)

Author keywords

Burst kinetics; Cellobiohydrolase; Cellulase; Degree of polymerization; Fluorescent polymeric model substrate; Processivity

Indexed keywords

ENZYME KINETICS; FLUORESCENCE; HYDROLYSIS; POLYMERIZATION; TITRATION;

BACTERIAL MICROCRYSTALLINE CELLULOSE; CELLOBIOHYDROLASE CEL7A; ENDOGLUCANASE CE15A; TRICHODERMA REESEI;

CELLULOSE;

ANTHRANILIC ACID DERIVATIVE; CELLULOSE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; CELLULOSE 1,4 BETA CELLOBIOSIDASE CEL6A; CELLULOSE 1,4 BETA CELLOBIOSIDASE CEL7A; GLUCAN SYNTHASE; GLUCAN SYNTHASE CEL5A; POLYMER; UNCLASSIFIED DRUG;

ARTICLE; DERIVATIZATION; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME SUBSTRATE; FLUORESCENCE; HYDROLYSIS; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; REACTION ANALYSIS; REDUCTION; TITRIMETRY; TRICHODERMA REESEI;

BINDING SITES; CARBOHYDRATE SEQUENCE; CELLULOSE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; FLUORESCENT DYES; FLUOROCARBON POLYMERS; FUNGAL PROTEINS; MODELS, THEORETICAL; POLYMERS; TITRIMETRY; TRICHODERMA;

ANTHRANILIC ACID; CELLULOSE; DEGREE OF POLYMERIZATION; ENZYMES; FLUORESCENCE; HYDROLYSIS; POLYMERIZATION; VOLUMETRY;

BACTERIA (MICROORGANISMS); HYPOCREA JECORINA; TRICHODERMA;

EID: 12844278017     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20041144     Document Type: Article

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