Single cell measurement of micro-viscosity by ratio imaging of fluorescence of styrylpyridinium probe

Biosensors and Bioelectronics

Volumn 20, Issue 9, 2005, Pages 1728-1736

  Wandelt, Barbara ^a   Cywinski, Piotr ^a   Darling, Graham D ^a   Stranix, Brent R ^a  

^a LODZ UNIVERSITY OF TECHNOLOGY   (Poland)

Author keywords

Cytoplasmic viscosity; Fluorescence; Molecular sensor; Single cell imaging

Indexed keywords

ESTERS; FLUORESCENCE; HYDROLYSIS; SPECTROSCOPIC ANALYSIS; TISSUE; VISCOSITY;

DUAL FLUORESCENCE; EMISSION BAND; FLUORESCENCE IMAGING MICROSCOPY; SINGLE CELL MEASUREMENT;

CELLS;

PYRIDINIUM DERIVATIVE; STYRYLPYRIDINIUM; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CALIBRATION; CONTROLLED STUDY; CYTOMETRY; EXCITABILITY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; HYDROLYSIS; MOLECULAR PROBE; NONHUMAN; RAT; SMOOTH MUSCLE FIBER; THORACIC AORTA; VISCOSITY;

ANIMALS; CELLS, CULTURED; FLOW CYTOMETRY; FLUORESCENT DYES; MICROFLUIDICS; MICROSCOPY, FLUORESCENCE; MOLECULAR PROBE TECHNIQUES; MUSCLE, SMOOTH, VASCULAR; PYRIDINIUM COMPOUNDS; RATS; SPECTROMETRY, FLUORESCENCE; VISCOSITY;

EID: 12844262985     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bios.2004.06.045     Document Type: Article

References (25)

1. D.R. Bessire, and E.L. Quitevis Effect of temperature and viscosity on rotational diffusion of Merocyanine 540 in polar solvents J. Phys. Chem. 98 1994 13083 13092
2. V.W. Burns Measurement of viscosity in living cells Biochem. Biophys. Res. Commun. 37 6 1969 1008 1014
3. V.W. Burns Microviscosity and calcium exchange in yeast cells and effects of phenyl alcohol Exp. Cell Res. 64 1971 35 40
4. J.H. Chamley-Campbell, G.R. Campbell, and R. Ross Phenotype-dependent response of cultured aortic smooth muscle to serum mitogens J. Cell Biol. 8 1981 379 383
5. Darling, G.D., Heisler, S., Stranix, B.R., Turkewitsch, P., Wandelt, B., 1998. Viscosity probe. US Patent 5 834 230.
6. J.A. Dix, and A.S. Verkman Mapping of fluorescence anisotropy in living cells by ratio imaging Biophys. J. 57 1990 231 240
7. K. Fushimi, and A.S. Verkman Low viscosity in the aqueous domain of cell cytoplasm measured by picosecond polarization microfluorimetry J. Cell Biol. 112 1991 719 725
8. J. Grabowski, K.-C. Hsiao, P.R. Baker, and C.H. Bornman Fluorogenic compound hydrolysis as a measure of toxicity-induced cytoplasmic viscosity and pH changes Environ. Pollut. 98 1 1998 1 5
9. K.Y. Law Fluorescence probe for microenvironments Chem. Phys. Lett. 75 1980 545 549
10. L.M. Loew, L.B. Cohen, J. Dix, E.N. Fluhler, V. Montana, G. Salama, and J.-Y. Wu A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations J. Membr. Biol. 130 1992 1 10
11. R.O. Loutfy, and B.A. Arnold Effect of viscosity and temperature on torsional relaxation of molecular rotors J. Phys. Chem. 86 1982 4205 4211
12. K. Luby-Phelps A novel fluorescence ratiometric method confirms the low solvent viscosity of the cytoplasm Biophys. J. 65 1993 236 242
13. K. Luby-Phelps, and R.A. Weisiger Role of cytoarchitecture in cytoplasmic transport Comp. Biochem. Physiol. 115B 3 1996 295 306
14. K. Luby-Phelps, D.L. Taylor, and F. Lanni Probing the structure of cytoplasm J. Cell Biol. 102 1986 2015 2022
15. A.M. Mastro, and A.D. Keith Diffusion in the aqueous compartment J. Cell Biol. 99 1984 180s 187s
16. H. Pin Kao, J.R. Abney, and A.S. Verkman Determinants of the translational mobility of a small solute in cell cytoplasm J. Cell Biol. 120 1 1993 175 194
17. N. Sakata, K. Kawamura, K. Fujimitsu, Y.Y. Chiang, and S. Takebayashi Immunocytochemistry of intermediate filaments in cultured arterial smooth muscle cells: differences in desmin and vimentin expression related to cell of origin and/or plating time Exp. Mol. Pathol. 53 1990 126 139
18. O. Skalli, W.S. Bloom, P. Ropraz, B. Azzarone, and G. Gabbiani Cytoskeletal remodeling of rat aortic smooth muscle cells in vitro: relationships to culture conditions and analogies to in vivo situations J. Submicrosc. Cytol. 18 1986 481 493
19. Stranix, B.R., 1997. Ph.D. Thesis, McGill University.
20. P. Turkewitsch, B. Wandelt, R.R. Ganju, G.D. Darling, and W.S. Powel Effect of nucleotides on the charge-transfer fluorescence of trans-4-(p-N,N-dimethylaminostyryl)-N-vinylbenzylpyridinium chloride Chem. Phys. Lett. 260 1996 142 146
21. P.A. Valberg, and J.P. Butler Intracellular movement and intracellular viscosity Comm. Theor. Biol. 2 2 1990 75 97
22. P.A. Valberg, and H.A. Feldman Magnetic particle motion within living cells Biophys. J. 52 1987 551 561
23. B. Wandelt, P. Turkewitsch, B.R. Stranix, and G.D. Darling Effect of temperature and viscosity on intramolecular charge-transfer fluorescence of a 4-p-dimethyaminostyrylpyridinium salt in protic solvents J. Chem. Soc., Faraday Trans. 1 91 1995 4199 4205
24. B. Wandelt, A. Mielniczak, P. Turkewitsch, B.R. Stranix, and G.D. Darling Substituted 4-[4-(dimethylamino)styryl]pyridinium salt as a fluorescent probe for cell microviscosity Biosens. Bioelectron. 18 2003 465 471
25. Weast, R.C. (Ed.), 1987. CRC Handbook of Chemistry and Physics, 68th ed. CRC Press Inc., Boca Raton, FL, USA, pp. D-221-D-269.