Substituted 4-[4-(dimethylamino)styryl]pyridinium salt as a fluorescent probe for cell microviscosity

Biosensors and Bioelectronics

Volumn 18, Issue 4, 2003, Pages 465-471

  Wandelt, Barbara ^a   Mielniczak, Alina ^a   Turkewitsch, Petra ^b   Darling, Graham D ^c   Stranix, Brent R ^d  

^a LODZ UNIVERSITY OF TECHNOLOGY   (Poland)

^b MCGILL UNIVERSITY   (Canada)

^c Active Materials Inc   (Canada)

^d Pharmacor Inc   (Canada)

Author keywords

Environmental sensitivity; Fluorescence; Intracellular viscosity; Microviscosity measurement

Indexed keywords

CELLS; COMPLEXATION; ESTERS; FLUORESCENCE; HYDROLYSIS; MIXTURES; MUSCLE; SALTS; SPECTROSCOPIC ANALYSIS; SUBSTITUTION REACTIONS; VISCOSITY;

DUAL FLUORESCENCE; EMISSION BANDS; FLUORESCENCE EMISSION; VISIBLE FLUORESCENCE SPECTROSCOPY;

NITROGEN COMPOUNDS;

4 [4 (DIMETHYLAMINO)STYRYL]PYRIDINIUM; ESTERASE; FLUORESCENT DYE; PHENAZOPYRIDINE; UNCLASSIFIED DRUG; 1 METHYL 4 (4 AMINOSTYRYL)PYRIDINIUM; 1-METHYL-4-(4-AMINOSTYRYL)PYRIDINIUM; PYRIDINIUM DERIVATIVE;

ANIMAL CELL; ARTICLE; CELL ACTIVITY; CELL CULTURE; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVITY; FLOW CYTOMETRY; FLUORESCENCE; HYDRODYNAMICS; HYDROLYSIS; IONIC STRENGTH; MEMBRANE PERMEABILITY; NONHUMAN; PH; RAT; SMOOTH MUSCLE FIBER; SPECTROFLUOROMETRY; VISCOMETRY; VISCOSITY; ANIMAL; CHEMISTRY; CULTURE TECHNIQUE; EVALUATION; INSTRUMENTATION; METABOLISM; METHODOLOGY; PHYSIOLOGY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY;

ANIMALS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CYTOPLASM; FLOW CYTOMETRY; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; MYOCYTES, SMOOTH MUSCLE; PYRIDINIUM COMPOUNDS; RATS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; VISCOSITY;

EID: 0642279486     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0956-5663(02)00156-2     Document Type: Article

References (25)

1. Abdel-Mottaleb, M.S.A., 1984. Fluorescence quantum yield and free rotor effect. Laser Chem. 4, 305-310.
2. Abdel-Mottaleb, M.S.A., Sherief, A.M.K., Ismaiel, L.F.M., De Schryver, F.C., Vanderauweraer, M.A., 1989. Photophysics of molecular rotors of functinalized surface-active styrylcyanine dyes. J. Chem. Soc. Faraday Trans. 2 (85), 1779-1788.
3. Bessire, D.R., Quitevis, E.L., 1994. Effect of temperature and viscosity on rotational diffusion of merocyanine 540 in polar solvents. J. Phys. Chem. 98, 13083-13092.
4. Burns, V. W., 1969. Measurement of viscosity in living cells. Biochem. Biophys. Res. Commun. 37 (6), 1008-1014.
5. Burns, V.W., 1971. Microviscosity and calcium exchange in yeast cells and effects of phenyl alcohol. Exp. Cell Res. 64, 35-40.
6. Chan, E.C.S., Stranix, B.R., Darling, G.D., Noble, P.E., 1996. A novel fluorochrome for the microscopic observation of microbial morphology in wet mounts. Can. J. Microbiol. 42, 875-879.
7. Darling, G.D., Heisler, S., Stranix, B.R., Turkewitsch, P., Wandelt, B., 1998. Viscosity probe. US Patent No 5 834-230.
8. Dix, J. A., Verkman, A.S., 1990. Mapping of fluorescence anisotropy in living cells by ratio imaging. Biophys. J. 57, 231-240.
9. Grabowski, Z.R., 1992. Electron transfer and the structural changes in the excited state. Pure Appl. Chem. 64 (9), 1249-1255.
10. Kiyohide, F., Verkman, A.S., 1991. Low viscosity in the aqueous domain of cell cytoplasm measured by picosecond polarization microfluorimetry. J. Cell Biol. 112, 719-725.
11. Lapouyade, K., Czeschka, W., Majenz, W., R3ettig, E., Gilabert, C., Rulliere, J., 1992. Photophysics of donor-acceptor substituted stilbenes. A time-resolved fluorescence study using selectively bridged dimethylamino cyano model compounds. J. Phys. Chem. 96, 9643-9650.
12. Loew, L.M., Cohen, L.B., Dix, J., Fluhler, E.N., Montana, V., Salama, G., Wu, J.-Y., 1992. A naphthyl analog of the aminostyryl pyridinium class of potentiometric membreane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations. J. Membr. Biol. 130, 1-103.
13. Loutfy, R.O., Arnold, B.A., 1982. Effect of viscosity and temperature on torsional relaxation of molecular rotors. J. Phys. Chem. 86, 4205-4211.
14. Luby-Phelps, K., 1993. A novel fluorescence ratiometric method confirms the low solvent viscosity of the cytoplasm. Biophys. J. 65, 236-242.
15. Luby-Phelps, K., 1994. Physical properties of cytoplasm. Cell Biol. 6, 3-9.
16. Mastro, A.M., Keith, A.D., 1984. Diffusion in the aqueous compartment. J. Cell Biol. 99, 180s-187s.
17. Parker, C.A., Rees, W.T., 1960. Correction of fluorescence spectra and measurement of fluorescence quantum efficiency. Analyst (London) 85, 587-600.
18. Pin Kao, H., Abney, J.R., Verkman, A.S., 1993. Determinants of the translational mobility of a small solute in cell cytoplasm. J. Cell Biol. 120(1), 175-194.
19. Stranix, B.R., 1997. Ph.D. thesis, McGill University.
20. Turkewitsch, P., Wandelt, B., Ganju, R.R., Darling, G.D., Powel, W.S., 1996. Effect of nucleotides on the charge-transfer fluorescence of trans-4-(p-N,N-dimethylaminostyryl)-N-vinylbenzylpyridinium chloride. Chem. Phys. Lett. 260, 142-146.
21. Turkewitsch, P., Wandelt, B., Darling, G.D., Powel, W.S., 1998. Nucleotides enhance the fluorescence of trans-4-(p-N,N-dimethylaminostyryl)-N- vinylbenzylpyridinium chloride. J. Photochem. Photobiol. A Chem. 117, 199-207.
22. Valberg, P.A., Feldman, H.A., 1987. Magnetic particle motion within living cells. Biophys. J. 52, 551-561.
23. Valberg, P.A., Butler, J.P., 1990. Intracellular movement and intracellular viscosity. Comm. Theor. Biol. 2 (2), 75-97.
24. Wandelt, B., Turkewitsch, P., Stranix, B.R., Darling, G.D., 1995. Effet of temperature and viscosity on intramolecular charge-transfer fluorescence of a 4-p-dimethyaminostyrylpyridinium salt in protic solvents. J. Chem. Soc. Faraday Trans. I 91, 4199-4205.
25. Weast, R.C. (Ed.), CRC Handbook of Chemistry and Physics, 68th ed., CRC Press, Boca Rator, FL, USA 1987, pp. D221-D269.