메뉴 건너뛰기




Volumn 39, Issue 4, 1996, Pages 249-258

Establishment of an in vitro Assay to Characterize Hepatitis C Virus NS3-4A Protease Trans-Processing Activity

Author keywords

Hepatitis C virus; In vitro; NS3 protease; NS4A; Trans cleavage assay

Indexed keywords

CYSTEINE; PROTEINASE; SERINE; VIRUS ENZYME;

EID: 12644261422     PISSN: 03005526     EISSN: 14230100     Source Type: Journal    
DOI: 10.1159/000150525     Document Type: Article
Times cited : (7)

References (43)
  • 3
    • 0000532607 scopus 로고
    • Epidemiology of viral hepatitis: An overview
    • Mast EE, Alter MJ: Epidemiology of viral hepatitis: An overview, Semin Virol 1993:4:273-283.
    • (1993) Semin Virol , vol.4 , pp. 273-283
    • Mast, E.E.1    Alter, M.J.2
  • 4
    • 0000361013 scopus 로고
    • Fields BN, Knipe DM, Howley PM (eds, Fields Virology, Philadelphia, Raven
    • Houghton M: Hepatitis C viruses; in Fields BN, Knipe DM, Howley PM (eds): Fields Virology, Philadelphia, Raven, 1995. vol I, pp 1035-1058.
    • (1995) Hepatitis C Viruses , vol.1 , pp. 1035-1058
    • Houghton, M.1
  • 5
    • 0025249362 scopus 로고
    • Hepatitis C virus shares amino acid sequence similarity with pes-tiviruses and flaviviruses as well as members of two plant virus supergroups
    • Miller RH, Purcell RH: Hepatitis C virus shares amino acid sequence similarity with pes-tiviruses and flaviviruses as well as members of two plant virus supergroups, Proc Natl Acad Sci USA 1990:87:2057-2061.
    • (1990) Proc Natl Acad Sci USA , vol.87 , pp. 2057-2061
    • Miller, R.H.1    Purcell, R.H.2
  • 6
    • 0001424128 scopus 로고
    • Flaviviridae: The viruses and their replication
    • Fields BN, Knipe DM, Howley PM (eds): Fields Virology, Philadelphia
    • Rice CM: Flaviviridae: The viruses and their replication: in Fields BN, Knipe DM, Howley PM (eds): Fields Virology, Philadelphia, Lip-pincott-Raven, 1995. vol I, pp931-960.
    • (1995) Lip-Pincott-Raven , vol.1 , pp. 931-960
    • Rice, C.M.1
  • 7
    • 0025991559 scopus 로고
    • Gene mapping of the putative structural region of the hepatitis C' virus genome by in vitro processing analysis
    • Hijikata M, Kato N, Ootsuyama Y, Nakagawa M, Shimotohno K: Gene mapping of the putative structural region of the hepatitis C' virus genome by in vitro processing analysis, Proc Natl Acad Sci USA 1991:88:5547-5551.
    • (1991) Proc Natl Acad Sci USA , vol.88 , pp. 5547-5551
    • Hijikata, M.1    Kato, N.2    Ootsuyama, Y.3    Nakagawa, M.4    Shimotohno, K.5
  • 9
    • 0028290389 scopus 로고
    • Processing of the hepatitis C virus E2-NS2 region: Identification of p7 and two distinct E2-specific products with different C termini
    • Lin C, Lindenbach BD, Pragai B, McC'ourt DW, Rice CM: Processing of the hepatitis C virus E2-NS2 region: Identification of p7 and two distinct E2-specific products with different C termini, J Virol 1994;68:5063-5073.
    • (1994) J Virol , vol.68 , pp. 5063-5073
    • Lin, C.1    Lindenbach, B.D.2    Pragai, B.3    McC'ourt, D.W.4    Rice, C.M.5
  • 11
    • 0027476809 scopus 로고
    • Expression and identification of hepatitis C virus polyprotcin cleavage products
    • Grakoui A, Wvchowski C, Lin C, Feinstone SM, Rice CM: Expression and identification of hepatitis C virus polyprotcin cleavage products, J Virol 1993;67:1385-1395.
    • (1993) J Virol , vol.67 , pp. 1385-1395
    • Grakoui, A.1    Wvchowski, C.2    Lin, C.3    Feinstone, S.M.4    Rice, C.M.5
  • 12
    • 0027414062 scopus 로고
    • Characterization of the hepatitis C virus-encoded serine proteinase: Determination of proteinase-dependent polv-protein cleavage sites
    • Grakoui A, McCourt DW, Wvchowski C, Feinstone SM, Rice CM: Characterization of the hepatitis C virus-encoded serine proteinase: Determination of proteinase-dependent polv-protein cleavage sites, J Virol 1993:67:2832-2843.
    • (1993) J Virol , vol.67 , pp. 2832-2843
    • Grakoui, A.1    McCourt, D.W.2    Wvchowski, C.3    Feinstone, S.M.4    Rice, C.M.5
  • 14
    • 0027163740 scopus 로고
    • Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus
    • Hijikata M, Mizushima H, Akagi T, Mori S, Kakiuchi N, Kato N, Tanaka T, Kimura K, Shimotohno K: Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus, J Virol 1993:67:4665-4675.
    • (1993) J Virol , vol.67 , pp. 4665-4675
    • Hijikata, M.1    Mizushima, H.2    Akagi, T.3    Mori, S.4    Kakiuchi, N.5    Kato, N.6    Tanaka, T.7    Kimura, K.8    Shimotohno, K.9
  • 16
    • 0027287798 scopus 로고
    • Nonstructural protein 3 of the hepatitis C virus encodes a serine-tvpc proteinase required for cleavage at the NS3/4 and NS4/5 junctions
    • Bartenschlager R, Ahlborn-Laakc L, Mous J, Jacobsen H: Nonstructural protein 3 of the hepatitis C virus encodes a serine-tvpc proteinase required for cleavage at the NS3/4 and NS4/5 junctions, J Virol 1993:67:3835-3844.
    • (1993) J Virol , vol.67 , pp. 3835-3844
    • Bartenschlager, R.1    Ahlborn-Laakc, L.2    Mous, J.3    Jacobsen, H.4
  • 17
    • 0028241489 scopus 로고
    • Kinetic and structural analyses of hepatitis C virus polyprotein processing
    • Bartenschlager R, Ahlborn-Laake L, Mous J, Jacobsen H: Kinetic and structural analyses of hepatitis C virus polyprotein processing, J Virol 1994:68:5045-5055.
    • (1994) J Virol , vol.68 , pp. 5045-5055
    • Bartenschlager, R.1    Ahlborn-Laake, L.2    Mous, J.3    Jacobsen, H.4
  • 19
    • 0027176287 scopus 로고
    • NS3 is a serine protease required for processing of hepatitis C virus polvprotcin
    • Tomei L, Failla C, Santolini E, DcFranccsco R, LaMonica N: NS3 is a serine protease required for processing of hepatitis C virus polvprotcin, J Virol 1993:67:4017-4026.
    • (1993) J Virol , vol.67 , pp. 4017-4026
    • Tomei, L.1    Failla, C.2    Santolini, E.3    Dcfranccsco, R.4    Lamonica, N.5
  • 21
    • 0028290579 scopus 로고
    • Both NS3 and NS4A arc required for proteolytic processing of hepatitis C virus nonstructural proteins
    • Failla C, Tomei L, De Francesco R: Both NS3 and NS4A arc required for proteolytic processing of hepatitis C virus nonstructural proteins, J Virol 1994:68:3753-3760.
    • (1994) J Virol , vol.68 , pp. 3753-3760
    • Failla, C.1    Tomei, L.2    De Francesco, R.3
  • 22
    • 0028089739 scopus 로고
    • Hepatitis C virus NS3 serine proteinase: Trans-clcavage requirements and processing kinetics
    • Lin C, Pragi BM, Grakoui A, Xu J, Rice CM: Hepatitis C virus NS3 serine proteinase: Trans-clcavage requirements and processing kinetics, J Virol 1994;68:8147-8157.
    • (1994) J Virol , vol.68 , pp. 8147-8157
    • Lin, C.1    Pragi, B.M.2    Grakoui, A.3    Xu, J.4    Rice, C.M.5
  • 24
    • 0029042438 scopus 로고
    • In vitro cleavage of hepatitis C virus polvprotein substrates by purified recombinant NS3 prolease
    • D'Souza EDA, Grace K, Sangar DV, Rowlands DJ, Clarke BE: In vitro cleavage of hepatitis C virus polvprotein substrates by purified recombinant NS3 prolease, J Gen Virol 1995:76: 1729-1736.
    • (1995) J Gen Virol , vol.76 , pp. 1729-1736
    • D'souza, E.1    Grace, K.2    Sangar, D.V.3    Rowlands, D.J.4    Clarke, B.E.5
  • 26
    • 0029094449 scopus 로고
    • The hepatitis C virus NS3 serine proteinase and NS4A cofactor: Establishment of a cell-free trans-processing assay
    • Lin C, Rice CM: The hepatitis C virus NS3 serine proteinase and NS4A cofactor: Establishment of a cell-free trans-processing assay, Proc Nall Acad Sci 1995;92:7622-7627.
    • (1995) Proc Nall Acad Sci , vol.92 , pp. 7622-7627
    • Lin, C.1    Rice, C.M.2
  • 27
    • 0029609892 scopus 로고
    • Recombinant baculovirus-expressed NS3 proteinase of hepatitis C virus shows activity in cell-based and in vitro assays
    • Overton H, McMillan D, Gillespie F, Mills J: Recombinant baculovirus-expressed NS3 proteinase of hepatitis C virus shows activity in cell-based and in vitro assays, J Gen Virol 1995;76:3009-3019.
    • (1995) J Gen Virol , vol.76 , pp. 3009-3019
    • Overton, H.1    McMillan, D.2    Gillespie, F.3    Mills, J.4
  • 29
    • 0026601458 scopus 로고
    • Sitc-dircctcd mutagenesis of virtually any plasmid by eliminating a unique site
    • Deng WP, Nickoloff JA: Sitc-dircctcd mutagenesis of virtually any plasmid by eliminating a unique site, Anal Biochem 1992;200:81-88.
    • (1992) Anal Biochem , vol.200 , pp. 81-88
    • Deng, W.P.1    Nickoloff, J.A.2
  • 31
    • 0025895589 scopus 로고
    • El A induces phosphorylation of the retinoblastoma protein independently of direct physical association between the El A and retinoblastoma products
    • Wang H-GH, Draetta G, Moran E: El A induces phosphorylation of the retinoblastoma protein independently of direct physical association between the El A and retinoblastoma products, Mol Cell Biol 1991; 11:4253-4265.
    • (1991) Mol Cell Biol , vol.11 , pp. 4253-4265
    • Wang, H.-G.1    Draetta, G.2    Moran, E.3
  • 32
    • 0028950450 scopus 로고
    • Sequence-dependent primer synthesis by the herpes simplex virus hclicase-primase complex
    • Tenney DJ, SheafTer AK, Hurlburt WW, Bifa-no M, Hamatake RK: Sequence-dependent primer synthesis by the herpes simplex virus hclicase-primase complex, J Biol Sci 1995:270: 9129-9136.
    • (1995) J Biol Sci , vol.270 , pp. 9129-9136
    • Tenney, D.J.1    Sheafter, A.K.2    Hurlburt, W.W.3    Bifa-No, M.4    Hamatake, R.K.5
  • 33
    • 0027512825 scopus 로고
    • Ribonuclcase S-peptide as a carrier in fusion proteins
    • Kim JS, Raines RT: Ribonuclcase S-peptide as a carrier in fusion proteins, Protein Sci 1993; 2/3:348-356.
    • (1993) Protein Sci , vol.2 , Issue.3 , pp. 348-356
    • Kim, J.S.1    Raines, R.T.2
  • 34
    • 0023664635 scopus 로고
    • Sequence from picomole quantities of proteins electroblottcd onto polyvinyli-dene difluoridc membranes
    • Matsudaira P: Sequence from picomole quantities of proteins electroblottcd onto polyvinyli-dene difluoridc membranes, J Biol Chem 1987; 262:10035-10038.
    • (1987) J Biol Chem , vol.262 , pp. 10035-10038
    • Matsudaira, P.1
  • 35
    • 0024590191 scopus 로고
    • Yellow fever virus proteins NS2A, NS2B, and NS4B: Identification and partial N-terminal amino acid sequence analysis
    • Chambers TJ, McCourt DW, Rice CM: Yellow fever virus proteins NS2A, NS2B, and NS4B: Identification and partial N-terminal amino acid sequence analysis, Virology 1989:169: 100-109.
    • (1989) Virology , vol.169 , pp. 100-109
    • Chambers, T.J.1    McCourt, D.W.2    Rice, C.M.3
  • 36
    • 0029074519 scopus 로고
    • A central region in the hepatitis C virus NS4A protein allows formation of an active NS3-NS4A serine proteinase complex in vivo and in vitro
    • Lin C, Thomson JA, Rice CM: A central region in the hepatitis C virus NS4A protein allows formation of an active NS3-NS4A serine proteinase complex in vivo and in vitro, J Virol 1995:69:4373-4380.
    • (1995) J Virol , vol.69 , pp. 4373-4380
    • Lin, C.1    Thomson, J.A.2    Rice, C.M.3
  • 37
    • 0028851296 scopus 로고
    • An amino-terminal domain of the hepatitis C virus NS3 proteasc is essential for interaction with NS4A
    • Failla C, Tomei L, DeFrancesco R: An amino-terminal domain of the hepatitis C virus NS3 proteasc is essential for interaction with NS4A, J Virol 1995:69:1769-1777.
    • (1995) J Virol , vol.69 , pp. 1769-1777
    • Failla, C.1    Tomei, L.2    Defrancesco, R.3
  • 38
    • 0029076837 scopus 로고
    • The N-terminal region of hepatitis C virus nonstructural protein 3 (NS3) is essential for stable complex formation with NS4A
    • Satoh S, Tanji Y, Hijikata M, Kimura K, Shi-motohno K: The N-terminal region of hepatitis C virus nonstructural protein 3 (NS3) is essential for stable complex formation with NS4A, J Virol 1995:69:4255-4260.
    • (1995) J Virol , vol.69 , pp. 4255-4260
    • Satoh, S.1    Tanji, Y.2    Hijikata, M.3    Kimura, K.4    Shi-Motohno, K.5
  • 39
    • 0028075902 scopus 로고
    • Substrate requirements of hepatitis C virus serine proteinase for intcrmolecular polypeptide cleavage in Escherichia coli
    • Komoda Y, Hijikata M, Sato S, Asabc S-I, Kimura K, Shimotohno K: Substrate requirements of hepatitis C virus serine proteinase for intcrmolecular polypeptide cleavage in Escherichia coli, J Virol 1994;68:7351-7357.
    • (1994) J Virol , vol.68 , pp. 7351-7357
    • Komoda, Y.1    Hijikata, M.2    Sato, S.3    Asabc, S.-I.4    Kimura, K.5    Shimotohno, K.6
  • 40
    • 0029655995 scopus 로고    scopus 로고
    • Identification of the sequence on NS4A required for enhanced cleavage of the NS5A/5B site by hepatitis C virus NS3 protease
    • Shimizu Y, Yamaji K, Masuho Y, Yokota T, Inouc H, Sudo K, Satoh S, Shimotohno K: Identification of the sequence on NS4A required for enhanced cleavage of the NS5A/5B site by hepatitis C virus NS3 protease, J Virol 1996:70/1:127-132.
    • (1996) J Virol , vol.70 , Issue.1 , pp. 127-132
    • Shimizu, Y.1    Yamaji, K.2    Masuho, Y.3    Yokota, T.4    Inouc, H.5    Sudo, K.6    Satoh, S.7    Shimotohno, K.8
  • 41
    • 0028820118 scopus 로고
    • Complex formation between the NS3 serine-type proteinase of the hepatitis C virus and NS4A and its importance for polvprotcin maturation
    • Bartenschlager R, Lohmann V, Wilkinson T, Koel JO: Complex formation between the NS3 serine-type proteinase of the hepatitis C virus and NS4A and its importance for polvprotcin maturation, J Virol 1995;69:7519-7528.
    • (1995) J Virol , vol.69 , pp. 7519-7528
    • Bartenschlager, R.1    Lohmann, V.2    Wilkinson, T.3    Koel, J.O.4
  • 42
    • 0028831056 scopus 로고
    • Hepatitis C virus-encoded nonstructural protein NS4A has versatile functions in viral protein processing
    • Tanji Y, Hijikata M, Satoh S, Kancko T, Shimotohno K: Hepatitis C virus-encoded nonstructural protein NS4A has versatile functions in viral protein processing, J Virol 1995:69: 1575-1581.
    • (1995) J Virol , vol.69 , pp. 1575-1581
    • Tanji, Y.1    Hijikata, M.2    Satoh, S.3    Kancko, T.4    Shimotohno, K.5
  • 43
    • 0029585447 scopus 로고
    • In vivo and in vitro trans-cleavage activity of hepatitis C virus serine proteinase expressed by recombinant baculoviruses
    • Suzuki T, Sato M, Chieda S, Shoji I, Harada T, Yamakawa Y, Watabe S, Matsuura Y, Miya-mura T: In vivo and in vitro trans-cleavage activity of hepatitis C virus serine proteinase expressed by recombinant baculoviruses, J Gen Virol 1995;76:3021-3029.
    • (1995) J Gen Virol , vol.76 , pp. 3021-3029
    • Suzuki, T.1    Sato, M.2    Chieda, S.3    Shoji, I.4    Harada, T.5    Yamakawa, Y.6    Watabe, S.7    Matsuura, Y.8    Miya-Mura, T.9


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.