Expression of biologically active kringle 5 domain of human plasminogen in Escherichia coli

Preparative Biochemistry and Biotechnology

Volumn 35, Issue 1, 2005, Pages 17-27

  Zhang, Hong Xia ^a   Fang, Lei ^a   Cheng, Jian ^a   Wei, Zheng ^a   Hua, Zi Chun ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

Endothelial cell; Fusion expression; Plasminogen kringle 5; Purification

Indexed keywords

HYBRID PROTEIN; PEPTIDE FRAGMENT; PLASMINOGEN; PLASMINOGEN KRINGLE 5;

ANIMAL; ARTICLE; BIOSYNTHESIS; CATTLE; CELL CULTURE; CELL PROLIFERATION; CHEMISTRY; CYTOLOGY; DOSE RESPONSE; DRUG EFFECT; ENDOTHELIUM CELL; ESCHERICHIA COLI; GENETICS; HUMAN; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING;

ANIMALS; CATTLE; CELL PROLIFERATION; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOTHELIAL CELLS; ESCHERICHIA COLI; HUMANS; PEPTIDE FRAGMENTS; PLASMINOGEN; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

BOVINAE; ESCHERICHIA COLI;

EID: 12444336960     PISSN: 10826068     EISSN: None     Source Type: Journal    
DOI: 10.1081/PB-200041433     Document Type: Article

References (18)

1. Claeys, H.; Sottrup-Jensen, L.; Zajdel, M.; Petersen, T.E.; Magnusson, S. Mutiple Gene duplication in the evolution of plasminogen: five regions of sequence homology with the two internally homologous structures in prothrombin. FEBS Lett. 1976, 61, 20-24.
2. Mulichak, A.M.; Tulinsky, A.; Ravichandran, K.G. Crystal and molecular structure of human plasminogen kringle 4 refined at 1.9-Å resolution. Biochemistry 1991, 30 (10), 576-588.
3. Angles-Cano, E.; Rojas, G. Apolipoprotein(a): structure-function relationship at the lysine-binding site and plasminogen activator cleavage site. Biol. Chem. 2002, 383, 93-99.
4. Ji, W.D.R.; Barrientos, L.G.; Llinas, M.; Gray, H.; Villarreal, X.; DeFord, M.E.; Castellino, F.J.; Kramer, R.A.; Trail, P.A. Selective inhibition by kringle 5 of human plasminogen on endothelial cell migration, an important process in angiogenesis. Biochem. Biophys. Res. Comm. 1998, 247, 414-419.
5. O'Reilly, M.S.; Holmgren, L.; Shing, Y.; Chen, C.; Rosenthal, R.A.; Moses, M.; Lane, W.S.; Cao, Y.; Sage, E.H.; Folkman, J. Angiostatin: a novel angiogenesis inhibitor that mediates the suppression of metastases by a lewis lung carcinoma. Cell 1994, 79, 315-328.
6. Cao, Y.H.; Ji, R.W.; Davidson, D.; Schaller, J.; Marti, D.; Sohndel, S.; McCance, S.G.; O'Reilly, M.S.; Llinas, M.; Folkman, J. Kringle domains of human angiostatin: characterization of the anti-proliferative activity on endothelial cells. J. Biol. Chem. 1996, 271 (29), 461-467.
7. Cao, Y.H.; Chen, A.; An, S.S.A.; Ji, R.W.; Davidson, D.; Cao, Y.M.; Llinas, M. Kringle 5 of plasminogen is a novel inhibitor of endothelial cell growth. J. Biol. Chem. 1997, 272 (22), 924-928.
8. Cao, R.H.; Wu, H.L.; Veitonmaki, N.; Linden, P.; Farnebo, J.; Shi, G.Y.; Cao, Y.H. Suppression of angiogenesis and tumor growth by the inhibitor K1-5 generated by plasmin-mediated proteolysis. Proc. Natl. Acad. Sci. USA 1999, 96 (5), 728-733.
9. Lu, H.; Dhanabal, M.; Volk, R.; Waterman, M.J.F.; Ramchandran, R.; Knebelmann, B.; Segal, M.; Sukhatme, V.P. Kringle 5 causes cell cycle arrest and apoptosis of endothelial cells. Biochem. Biophys. Res. Comm. 1999, 258, 668-673.
10. Stathakis, P.; Lay, A.J.; Fitzgerald, M.; Schlieker, C.; Matthias, L.J.; Hogg, P.J. Angiostatin formation involves disulfide bond reduction and proteolysis in kringle 5 of plasmin. J. Biol. Chem. 1999, 274 (8), 910-916.
11. Zhang, D.; Kaufman, P.L.; Gao, G.; Saunders, R.A.; Ma, J.X. Intravitreal injection of plasminogen kringle 5, an endogenous angiogenic inhibitor, arrests retinal neovascularization in rats. Diabetologia 2001, 44, 757-765.
12. Lu, H.; Zhang, H.; Wang, Q.; Yuan, H.; He, W.; Zhao, Z.; Li, Y. Purification, refolding of hybrid hIFNgamma-kringle 5 expressed in Escherichia coli. Curr. Microbiol. 2001, 42, 211-216.
13. Lu, H.; Yuan, H.; Li, Y. Expression of human plasminogen kringle 5 as fusion protein with truncated hIFNgamma gene in Escherichia coli. J. Biotechnol. 2002, 94, 277-285.
14. Hua, Z.C.; Fu, H.L.; Chen, Y.H.; Yu, R.R.; Wang, J.; Zhu, D.X. Synthesis and expression of a gene for kringle-2 domain of tissue plasminogen activator in E. coli. Sci. China (B) 1994, 37, 667-676.
15. Cleary, S.; Mulkerrin, M.G.; Kelley, R.F. Purification and characterization of tissue plasminogen activator kringle-2 domain expressed in Escherichia coli. Biochemistry 1989, 28, 1884-1891.
16. Xu, Z.; Yuan, L.D.; Zhu, N.; Hua, Z.C.; Wang, X.C. Cloning, expression and purification of kringle 1 domain of human plasminogen in Escherichia coli. J. Nanjing Univ. (Natl Sci.) 2000, 36, 508-510.
17. Hrzenjak, A.; Frank, S.; Maderegger, B.; Sterk, H.; Kostner, G.M. Apo(a)-kringle IV-type 6: expression in Escherichia coli, purification and in vitro refolding. Protein Eng. 2000, 13, 661-666.
18. Zhu, M.; Cheng, J.; Hua, Z.C. Expression of kringle 5 domain of human plasminogen in Pichia postoris. Prep. Biochem. Biotechnol. 2003, 33, 269-281.