In vivo confocal laser scanning microscopy and micropuncture in intact rat

Nephron - Experimental Nephrology

Volumn 99, Issue 1, 2005, Pages

  Ohno, Yoshio ^a   Birn, Henrik ^a   Christensen, Erik I ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

Confocal laser scanning microscopy; Endocytosis; Intravital microscopy; Kidney proximal tubules; Protein reabsorption

Indexed keywords

ALBUMIN; TRANSFERRIN;

ARTICLE; BIOACCUMULATION; CELL VACUOLE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ENDOCYTOSIS; FLUORESCENCE ANALYSIS; IMAGE ANALYSIS; IN VIVO STUDY; INFUSION; KIDNEY PROXIMAL TUBULE; LYSOSOME; MALE; NEPHROLOGY; NONHUMAN; PRIORITY JOURNAL; PUNCH BIOPSY; RAT; THERMOSTABILITY;

ALBUMINS; ANIMALS; ENDOCYTOSIS; KIDNEY TUBULES, PROXIMAL; LIGANDS; MALE; MICROSCOPY, CONFOCAL; PUNCTURES; RATS; RATS, WISTAR; TRANSFERRIN;

EID: 12444330895     PISSN: 16602129     EISSN: None     Source Type: Journal    
DOI: 10.1159/000081794     Document Type: Article

References (19)

1. Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T: Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr Biol 1995;5:635-642.
2. Baumann CT, Lim CS, Hager GL: Simultaneous visualization of the yellow and green forms of the green fluorescent protein in living cells. J Histochm Cytochem 1998;46:1073-1076.
3. Messerli JM, Perriard JC: Three-dimensional analysis and visualization of myofibrillogenesis in adult cardiomyocytes by confocal microscopy. Microsc Res Tech 1995;30:521-530.
4. Sönnichsen B, Renzis SD, Nielsen E, Rietdorf J, Zerial M: Distinct membrane domains on endosomes in the recycling pathway visualized by multicolour imaging of Rab4, Rab5, and Rab 11. J Cell Biol 2000;149:902-913.
5. Roberts RL, Barbieri MA, Pryse KM, Chua M, Morisaki JH, Stahl PD: Endosome fusion in living cells overexpressing GFP-rab5. J Cell Sci 1999;112:3667-3675.
6. Huang F, Nesterov A, Carter RE, Sorkin A: Trafficking of yellow-fluorescent-protein-tagged μ1 subunit of clathrin adaptor AP-1 complex in living cells. Traffic 2001;2:345-357.
7. Sako Y, Minoguchi S, Yanagida T: Single-molecule imaging of EGFR signalling on the surface of living cells. Nat Cell Biol 2000;2:168-172.
8. Kleinfeld D, Mitra PP, Helmchen F, Denk W: Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex. Proc Natl Acad Sci USA 1998;95:15741-15746.
9. Grayson MH, Chaplin DD, Karl IE, Hotchkiss RS: Confocal fluorescent intravital microscopy of the murine spleen. J Immunol Methods 2001;256:55-63.
10. Huzaira M, Rius F, Rajadhyaksha M, Anderson RR, Gonzalez S: Topographic variations in normal skin, as viewed by in vivo reflectance confocal microscopy. J Invest Dermatol 2001;116:846-852.
11. Jester JV, Lee YG, Li J, Chakravarti S, Paul J, Petroll WM, Cavanagh HD: Measurement of corneal sublayer thickness and transparency in transgenic mice with altered corneal clarity using in vivo confocal microscopy. Vision Res 2001;41:1283-1290.
12. Schneider P, Foitzik T, Kahrau S, Podufal A, Buhr H-J: An experimental rat model for studying pulmonary microcirculation by in vivo videomicroscopy. Microvasc Res 2001;62:421-434.
13. Boyde A, Capasso G, Unwin RJ: Conventional and confocal epi-reflection and fluorescence microscopy of the rat kidney in vivo. Exp Nephrol 1998;6:398-408.
14. Dunn KW, Sandoval RM, Kelly KJ, Dagher PC, Tanner GA, Atkinson SJ, Bacallao RL, Molitoris BA: Functional studies of the kidney of living animals using multicolour two-photon microscopy. Am J Physiol Cell Physiol 2002;283:C905-C916.
15. Christensen EI, Rennke HG, Carone FA: Renal tubular uptake of protein: Effect of molecular charge. Am J Physiol 1983;244:F436-F441.
16. Birn H, Nielsen S, Christensen EI: Internalization and apical-to-basolateral transport of folate in rat kidney proximal tubule. Am J Physiol 1997;272:F70-F78.
17. Christensen EI, Birn H: Megalin and cubilin: Multifunctional endocytic receptors. Nat Rev Mol Cell Biol 2002;3:256-266.
18. Christensen EI: Rapid protein uptake and digestion in proximal tubule lysosomes. Kidney Int 1976;10:301-310.
19. 125I-labeled homologous albumin by rat kidney proximal tubule cells: A study of microperfused single proximal tubules by electron microscopic autoradiography and histochemistry. J Ultrastruct Res 1966;15:197-241.