Patterns of K-ras codon 12 and 13 mutations found in pancreatic adenocarcinoma of 30 Chinese patients by microdissection, PCR and direct sequencing

Journal of Gastroenterology and Hepatology (Australia)

Volumn 20, Issue 1, 2005, Pages 67-72

  Wei, Shuanzeng ^a   Liang, Zhiyong ^a   Gao, Jie ^a   Wu, Shafei ^a   Zhu, Hong ^a   Liu, Hongrui ^a   Liu, Tonghua ^a  

^a PEKING UNION MEDICAL COLLEGE HOSPITAL   (China)

Author keywords

K ras; Mutation; Pancreatic adenocarcinoma; Polymerase chain reaction; Sequence

Indexed keywords

ADULT; AGED; ARTICLE; CHINESE; CLINICAL ARTICLE; CODON; CONTROLLED STUDY; FEMALE; GENE MUTATION; GENE SEQUENCE; GENETIC ANALYSIS; HUMAN; HUMAN TISSUE; MALE; MICRODISSECTION; ONCOGENE K RAS; PANCREAS ADENOCARCINOMA; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS;

EID: 12444297635     PISSN: 08159319     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1440-1746.2004.03542.x     Document Type: Article

References (16)

1. Berndt C, Haubold K, Wenger F et al. K-ras mutations in stools and tissue samples from patients with malignant and nonmalignant pancreatic diseases. Clin. Chem. 1998; 44: 2103-7.
2. Slebos RJC, Hoppin JA, Tolbert PE et al. K-ras and p53 in pancreatic cancer: association with medical history, histopathology, and environmental exposures in a population-based study. Cancer Epidemiol. Biomarkers Prev. 2000; 9: 1223-32.
3. Porta M, Malats N, Guarner L et al. Association between coffee drinking and K-ras mutations in exocrine pancreatic cancer. J. Epidemiol. Community Health 1999; 53: 702-9.
4. Wang JY, Lian ST, Chen YF et al. Unique K-ras mutational pattern in pancreatic adenocarcinoma from Taiwanese patients. Cancer Lett. 2002; 180: 153-8.
5. Scarpa A, Capelli P, Villanueva A et al. Pancreatic cancer in Europe: K-ras gene mutation pattern shows geographical differences. Int. J. Cancer 1994; 7: 176-1.
6. Moor PS, Sipos B, Orlandini S. Genetic profile of 22 pancreatic carcinoma cell lines. Virchows Arch. 2001; 9: 798-802.
7. Aoki Y, Hosaka S, Tachibana N, Karasawa Y, Kawa S, Kiyosawa K. Reassessment of K-ras mutations at codon 12 by direct PCR and sequencing from tissue microdissection in human pancreatic adenocarcinomas. Pancreas 2000; 21: 152-7.
8. Chang MC, Chang YT, Wu MS, Shun CT, Tien YW, Lin JT. K-ras mutation at codon 12 in stage I pancreatic adenocarcinoma: analysis by laser capture microdissection and direct sequencing. J. Formos. Med. Assoc. 2001; 100: 352-4.
9. Song MM, Nio Y, Dong M et al. Comparison of K-ras point mutations at codon 12 and p21 expression in pancreatic cancer between Japanese and Chinese patients. J. Surg. Oncol. 2000; 75: 176-85.
10. Motojima K, Urano T, Nagata Y, Shiku H, Tsunoda T, Kanematsu T. Mutations in the Kirsten-ras oncogene are common but lack correlation with prognosis and tumor stage in human pancreatic carcinoma. Am. J. Gastroenterol. 1991; 86: 1784-8.
11. Nagata Y, Abe M, Motoshima K, Nakayama E, Shiku H. Frequent glycine-to-aspartic acid mutations at codon 12 of c-Ki-ras gene in human pancreatic cancer in Japanese. Jpn J. Cancer Res. 1990; 81: 135-40.
12. Mariyama M, Kishi K, Nakamura K, Obata H, Nishimura S. Frequency and types of point mutation at the 12th codon of the c-Ki-ras gene found in pancreatic cancers from Japanese patients. Jpn J. Cancer Res. 1989; 80: 622-6.
13. Smit VT, Boot AJ, Smits AM, Fleuren GJ, Cornelisse CJ, Bos JL. KRAS codon 12 mutations occur very frequently in pancreatic adenocarcinomas. Nucleic Acids Res. 1988; 16: 7773-82.
14. Grunewald K, Lyons J, Frohlich A et al. High frequency of Ki-ras codon 12 mutations in pancreatic adenocarcinomas. Int. J. Cancer 1989; 43: 1037-41.
15. Lemoine NR, Jain S, Hughes CM et al. Ki-ras oncogene activation in preinvasive pancreatic cancer. Gastroenterology 1992; 102: 230-6.
16. Li DH, Firozi PF, Zhang WQ et al. DNA adducts, genetic polymorphisms, and K-ras mutation in human pancreatic cancer. Mutat. Res. 2002; 513: 37-48.