Monitoring the microbial population dynamics at the start-up stage of wastewater treatment reactor by terminal restriction fragment length polymorphism analysis based on 16s rDNA and rRNA gene sequences

Journal of Bioscience and Bioengineering

Volumn 98, Issue 6, 2004, Pages 425-428

  Terahara, Takeshi ^a   Hoshino, Tatsuhiko ^a   Tsuneda, Satoshi ^a   Hirata, Akira ^a   Inamori, Yuhei ^b  

^a WASEDA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES   (Japan)

Author keywords

Microbial population dynamics; Multidimensional scaling (MDS); Start up stage; Terminal restriction fragment length polymorphism (T RFLP); Wastewater treatment reactor

Indexed keywords

BACTERIA; CONDITION MONITORING; DNA SEQUENCES; ENVIRONMENTAL ENGINEERING; GENES; NITRIFICATION; RNA;

MULTIDIMENSIONAL SCALING (MDS) ANALYSIS; NITRIFYING BACTERIA; POLYMORPHISM; WASTEWATER TREATMENT REACTOR;

WASTEWATER TREATMENT;

RIBOSOME DNA; RIBOSOME RNA;

ARTICLE; BACTERIAL COUNT; DISPERSION; ENVIRONMENTAL FACTOR; GENE SEQUENCE; GENETIC VARIABILITY; MICROBIAL POPULATION DYNAMICS; MICROFLORA; NITRIFICATION; NONHUMAN; REACTOR; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; WASTE WATER MANAGEMENT; WASTEWATER TREATMENT REACTOR;

BACTERIA (MICROORGANISMS);

EID: 12444293836     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(05)00307-5     Document Type: Article

References (12)

1. Amann, R., Lemmer, H., and Wagner, M.: Monitoring the community structure of wastewater treatment plants: a comparison of old and new techniques. FEMS Microbiol. Ecol., 25, 205-215 (1998).
2. Blackall, L. L., Burrell, P. C., Gwilliam, H., Bradford, D., Bond, P. C., and Hugenholz, P.: The use of 16S rDNA clone libraries to describe the microbial diversity of activated sludge communities. Water Sci. Technol., 37, 451-454 (1998).
3. Godon, J. J., Zumstein, E., Dabert, P., Habouzit, F., and Moletta, R.: Molecular microbial diversity of an anaerobic digestor as determined by small-subunit rDNA sequence analysis. Appl. Environ. Microbiol., 63, 2802-2813 (1997).
4. Snaidr, J., Amann, R., Huber, I., Ludwig, W., and Schleifer, K. H.: Phylogenetic analysis and in situ identification of bacteria in activated sludge. Appl. Environ. Microbiol., 63, 2884-2896 (1997).
5. Liu, W., Marsh, T. L., Cheng, H., and Forney, L. J.: Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl. Environ. Microbiol., 63, 4516-4522 (1997).
6. Osborn, A. M., Moore, E. R., and Timmis, K. N.: An evaluation of terminal-restriction fragment length polymorphism (T-RPLP) analysis for the study of microbial community structure and dynamics. Environ. Microbiol., 2, 39-50 (2000).
7. Scab, D. J. and Kerkhof, L. J.: Horizontal heterogeneity of denitrifying bacterial communities in marine sediments by terminal restriction fragment length polymorphism analysis. Appl. Environ. Microbiol., 66, 1980-1986 (2000).
8. Dunbar, J., Ticknor, L. O., and Kuske, C. R.: Assessment of microbial diversity in four southwestern United States soils by 16S rRNA gene terminal restriction fragment analysis. Appl. Environ. Microbiol., 66, 2943-2950 (2000).
9. Lukow, T., Dunfield, P. F., and Liesack, W.: Use of the T-RFLP technique to assess spatial and temporal changes in the bacterial community structure within an agricultural soil planted with transgenic and non-transgenic potato plants. FEMS Microbiol. Ecol., 32, 241-247 (2000).
10. Dollhopf, S. L., Hashsham, S. A., and Tiedje, J.: Interpreting 16S rDNA T-RFLP data: application of self-organizing maps and principal component analysis to describe community dynamics and convergence. Microb. Ecol., 42, 495-505 (2001).
11. Poly, F., Ranjard, L., Nazaret, S., Gourbiere, F., and Monrozier, L. J.: Comparison of nifH gene pools in soils and soil microenvironments with contrasting properties. Appl. Environ. Microbiol., 67, 2255-2262 (2001).
12. Marsh, T. L., Saxman, P., Cole, J., and Tiedje, J.: Terminal restriction fragment length polymorphism analysis program, a web-based research tool for microbial community analysis. Appl. Environ. Microbiol., 66, 3616-3620 (2000).