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Bioorganic and Medicinal Chemistry Letters
Volumn 13, Issue 9, 2003, Pages 1565-1570
Controlling the intracellular localization of fluorescent polyamide analogues in cultured cells
Author keywords

[No Author keywords available]
Indexed keywords

FLUORESCEIN; POLYAMIDE; VERAPAMIL;
EID: 12444266726     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(03)00152-5     Document Type: Article
Times cited : (47)
References (18)
  • 6
    • 0030059085 scopus 로고    scopus 로고
    • The polyamides were labeled with BODIPY-FL-X, SE and FITC respectively using standard labeling conditions
    • Baird E., Dervan P. J. Am. Chem. Soc. 118:1996;6141. The polyamides were labeled with BODIPY-FL-X, SE and FITC respectively using standard labeling conditions.
    • (1996) J. Am. Chem. Soc. , vol.118 , pp. 6141
    • Baird, E.1    Dervan, P.2
  • 7
    • 84994433459 scopus 로고    scopus 로고
    • 5) were plated on 25-mm round glass coverslips in 30-mm wells and incubated for 24 h to allow cells to adhere. Fluorescent polyamides were freshly prepared in DMSO to 10 mM, diluted to 1 mM with distilled water, added pre-warmed cell culture media to a final concentration 10 μM polyamide, 0.1% DMSO, and cells were incubated in this media for an additional 16 h as before. Where applicable, cells were also pretreated with one of the MDR inhibitors, verapamil, bepridil, cyclosporin A, or ketoconazole, at a concentration of 5-100 μM for 30 min before the addition of the fluorescent polyamides
    • 5) were plated on 25-mm round glass coverslips in 30-mm wells and incubated for 24 h to allow cells to adhere. Fluorescent polyamides were freshly prepared in DMSO to 10 mM, diluted to 1 mM with distilled water, added pre-warmed cell culture media to a final concentration 10 μM polyamide, 0.1% DMSO, and cells were incubated in this media for an additional 16 h as before. Where applicable, cells were also pretreated with one of the MDR inhibitors, verapamil, bepridil, cyclosporin A, or ketoconazole, at a concentration of 5-100 μM for 30 min before the addition of the fluorescent polyamides.
  • 8
    • 84994426514 scopus 로고    scopus 로고
    • Live wet mounted cells were examined and photographed using an Olympus AX70 microscope equipped with fluorescence optics and a Sony 3CCD color video camera. DAPI was detected using a bandpass 405±20 nm excitation filter, a 420 nm dichroic beam splitter, and a ≥450 emission filter (DAPI filter set). BODIPY and Fluorescein conjugated polyamides were selectively detected using a bandpass 485±11 nm excitation filter, a 505 nm dichroic beam splitter, and a 530±15 nm emission filter (fluorescein filter set). Organelle-specific probes were detected using a 546±5 nm excitation filter, a 570 nm dichroic beam splitter and a 590 nm longpass emission filter (rhodamine filter set)
    • Live wet mounted cells were examined and photographed using an Olympus AX70 microscope equipped with fluorescence optics and a Sony 3CCD color video camera. DAPI was detected using a bandpass 405±20 nm excitation filter, a 420 nm dichroic beam splitter, and a ≥450 emission filter (DAPI filter set). BODIPY and Fluorescein conjugated polyamides were selectively detected using a bandpass 485±11 nm excitation filter, a 505 nm dichroic beam splitter, and a 530±15 nm emission filter (fluorescein filter set). Organelle-specific probes were detected using a 546±5 nm excitation filter, a 570 nm dichroic beam splitter and a 590 nm longpass emission filter (rhodamine filter set).
  • 10
    • 84994441330 scopus 로고    scopus 로고
    • 2XRos, LysToracker® Red DND-99, or BODIPY® TR ceramide was added directly to the cell culture for 15 min to 1 h as recommended by the supplier (Molecular Probes). Just prior to examination 4,6-diamidino-2-phenylindole, dihydrochloride (DAPI) was added to cell cultures to 300 nM and samples were incubated for at least 5 min at room temperature. The coverslips were rinsed several times in PBS and wet mounted
    • 2XRos, LysToracker® Red DND-99, or BODIPY® TR ceramide was added directly to the cell culture for 15 min to 1 h as recommended by the supplier (Molecular Probes). Just prior to examination 4,6-diamidino-2-phenylindole, dihydrochloride (DAPI) was added to cell cultures to 300 nM and samples were incubated for at least 5 min at room temperature. The coverslips were rinsed several times in PBS and wet mounted.

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.