Gene disruption in Trichoderma atroviride via Agrobacterium-mediated transformation

Current Genetics

Volumn 45, Issue 1, 2004, Pages 54-60

  Zeilinger, Susanne ^a  

^a VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Agrobacterium mediated transformation; Gene disruption; Mycoparasitism; Trichoderma atroviride

Indexed keywords

ESCHERICHIA COLI PROTEIN; HETEROTRIMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; HYGROMYCIN; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN HPH; PROTEIN SUBUNIT; UNCLASSIFIED DRUG;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ALPHA CHAIN; ARTICLE; COLONY FORMATION; CONIDIUM; CONTROLLED STUDY; CULTURE MEDIUM; DNA FLANKING REGION; FUNGAL STRAIN; GENE CONSTRUCT; GENE DISRUPTION; GENE FREQUENCY; GENE LOCUS; GENE LOSS; GENE TARGETING; GENE VECTOR; GENETIC CODE; GENETIC MARKER; GENETIC SELECTION; GENETIC TRANSCRIPTION; GENETIC TRANSFORMATION; HOMOLOGOUS RECOMBINATION; MOLECULAR CLONING; MYCOPARASITISM; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHIZOBIUM; SIGNAL TRANSDUCTION; TRICHODERMA;

GENE TARGETING; GENES, FUNGAL; GENETIC VECTORS; MUTAGENESIS, INSERTIONAL; MUTAGENESIS, SITE-DIRECTED; RECOMBINATION, GENETIC; RHIZOBIUM; RNA, FUNGAL; RNA, MESSENGER; TRANSFORMATION, GENETIC; TRICHODERMA;

AGROBACTERIUM; ESCHERICHIA COLI; FUNGI; NEGIBACTERIA; TRICHODERMA; TRICHODERMA ATROVIRIDE; VECTORS;

EID: 1242319453     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-003-0454-8     Document Type: Article

References (24)

1. Bird D, Bradshaw R (1997) Gene targeting is locus dependent in the filamentous fungus Aspergillus nidulans. Mol Gen Genet 255:219-225
2. Bundock P, Den Dulk-Ras A, Beijersbergen AGM, Hooykaas PJJ (1995) Trans-kingdom transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J 14:3206-3214
3. Brunner K, Peterbauer C, Mach RL, Lorito M, Zeilinger S, Kubicek CP (2003) The Nag1 N-acetylglucosaminidase of Trichoderma atroviride is essential for chitinase induction by chitin and of major relevance to biocontrol. Curr Genet 43:289-295
4. Carsolio C, Benhamou N, Haran S, Cortes C, Gutierrez A, Chet I, Herrera-Estrella A (1999) Role of the Trichoderma harzianum endochitinase gene, ech42, in mycoparasitism. Appl Environ Microbiol 65:929-935
5. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159
6. Covert SF, Kapoor P, Lee M, Briley A, Nairn CJ (2001) Agrobacterium tumefaciens-mediated transformation of Fusarium circinatum. Mycol Res 105:259-264
7. De Groot MJA, Bundock P, Hooykaas PJJ, Beijersbergen AGM (1998) Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol 16:839-842
8. Gouka, RJ, Gerk C, Hooykaas PJJ, Bundock P, Musters W, Verrips CT, De Groot MJA (1999) Transformation of Aspergillus awamori by Agrobacterium tumefaciens-mediated homologous recombination. Nat Biotechnol 17:598-601
9. Gruber F, Visser J, Kubicek CP, De Graaff LH (1990) The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain. Curr Genet 18:71-76
10. Harman GE, Björkman T (1998) Potential and existing uses of Trichoderma and Gliocladium for plant disease control and plant growth enhancement. In: Harman GE, Kubicek CP (eds) Trichoderma and Gliocladium, vol 2. Taylor and Francis, London, pp 229-265
11. Kubicek CP, Mach RL, Peterbauer CK, Lorito M (2001) Trichoderma: from genes to biocontrol. J Plant Pathol 83:11-23
12. Kullnig CM, Krupica T, Woo SL, Mach RL, Rey M, Benitez T, Lorito M, Kubicek CP (2001) Confusion abounds over identity of Trichoderma biocontrol isolates. Mycol Res 105:70-72
13. Malonek S, Meinhardt F (2001) Agrobacterium tumefaciens-mediated genetic transformation of the phytopathogenic ascomycete Calonectria morganii. Curr Genet 40:152-155
14. Mozo T, Hooykaas PJ (1991) Electroporation of megaplasmids into Agrobacterium. Plant Mol Biol 16:917-918
15. Mukherjee PK, Latha J, Hadar R, Horwitz B. (2003) TmkA, a mitogen-activated protein kinase of Trichoderma virens, is involved in biocontrol properties and repression of conidiation in the dark. Eukaryot Cell 2:446-455
16. Mullins ED, Chen X, Romaine P, Raina R, Geiser DM, Kang S (2001) Agrobacterium-mediated transformation of Fusarium oxysporum: an efficient tool for insertional mutagenesis and gene transfer. Phytopathology 91:173-180
17. Peterbauer C, Litscher D, Kubicek CP (2002) The Trichoderma atroviride seb1 (stress response element binding) gene encodes an AGGGG-binding protein which is involved in the response to high osmolarity stress. Mol Gen Genomics 268:223-231
18. Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, Hondel CAMJJ van den (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56:117-124
19. Rocha-Ramirez V, Omero C, Chet I, Horwitz BA, Herrera-Estrella A (2002) Trichoderma atroviride G-protein α-subunit gene tga1 is involved in mycoparasitic coiling and conidiation. Eukaryot Cell 1:594-605
20. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
21. Suominen PL, Mäntylä AL, Karhunen T, Hakola S, Nevalainen H (1993) High frequency one-step gene replacement in Trichoderma reesei. II. Effects of deletions of individual cellulase genes. Mol Gen Genet 241:523-530
22. Woo S, Donzelli B, Scala F, Mach RL, Harman GE, Kubicek CP, Del Sorbo G, Lorito M (1999) Disruption of the ech42 (endochitinase-encoding) gene affects biocontrol activity in Trichoderma harzianum P1. Mol Plant-Microbe Interact 12:419-429
23. Zhang A, Lu P, Dahl-Roshak AM, Paress PS, Kennedy S, Tkacz JS, An Z (2003) Efficient disruption of a polyketide synthase gene (pks1) required for melanin synthesis through Agrobacterium-mediated transformation of Glarea lozoyensis. Mol Gen Genomics 268:645-655
24. Zwiers L-H, De Waard MA (2001) Efficient Agrobacterium tumefaciens-mediated gene disruption in the phytopathogen Mycosphaerella graminicola. Curr Genet 39:388-393