Identification of a gene encoding a putative phosphotransferase system enzyme IIBC in Listeria welshimeri and its application for diagnostic PCR

Letters in Applied Microbiology

Volumn 38, Issue 2, 2004, Pages 151-157

  Liu, D ^a   Ainsworth, A J ^a   Austin, F W ^a   Lawrence, M L ^a  

^a MISSISSIPPI STATE UNIVERSITY   (United States)

Author keywords

Dot blot hybridization; Listeria welshimeri; PCR; Phosphotransferase; ScrA; Sucrose fermentation

Indexed keywords

CLONING; ENZYMES; GENE ENCODING; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION;

DOT-BLOT HYBRIDIZATION; GENES ENCODING; GENOMIC DNA; LISTERIA SPECIES; LISTERIA WELSHIMERI; PCR; PHOSPHOTRANSFERASE SYSTEM; PHOSPHOTRANSFERASES; SCRA; SUCROSE FERMENTATION;

DISEASES;

BACTERIAL ENZYME; GENOMIC DNA; PHOSPHOTRANSFERASE; PRIMER DNA; PROTEIN IIBC; UNCLASSIFIED DRUG;

BACTERIUM; GENE EXPRESSION; IDENTIFICATION METHOD; POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; COMPARATIVE STUDY; CONTROLLED STUDY; DOT HYBRIDIZATION; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC CODE; LISTERIA WELSHIMERI; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; RNA TRANSLATION; SEQUENCE ANALYSIS; SPECIES DIFFERENTIATION; TECHNIQUE;

BACTERIA (MICROORGANISMS); LISTERIA; LISTERIA WELSHIMERI; NEGIBACTERIA; POSIBACTERIA;

EID: 1242315413     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2003.01466.x     Document Type: Article

References (24)

1. Abrahim, A., Papa, A., Soultos, N., Ambrosiadis, I. and Antoniadis, A. (1998) Antibiotic resistance of Salmonella spp. and Listeria spp. isolates from traditionally made fresh sausages in Greece. Journal of Food Protection 61, 1378-1380.
2. Aznar, R. and Alarcon, B. (2002) On the specificity of PCR detection of Listeria monocytogenes in food: a comparison of published primers. Systematic and Applied Microbiology 25, 109-119.
3. Bassler, H.A., Flood, S.J., Livak, K.J., Marmaro, J., Knorr, R. and Batt, C.A. (1995) Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes. Applied and Environmental Microbiology 61, 3724-3728.
4. Batt, C.A. (2000) Listeria/Introduction. In Encyclopedia of Food Microbiology, Vol. 2 ed. Robinson, R.K., Batt, C.A. and Patel, P.D. pp. 1195-1198. London: Academic Press.
5. Biais, B.W., Turner, G., Sooknanan, R. and Malek, L.T. (1997) A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences. Applied and Environmental Microbiology 63, 310-313.
6. Bohnert, M., Dilasser, F., Dalet, C., Mengaud, J. and Cossart, P. (1992) Use of specific oligonucleotides for direct enumeration of Listeria monocytogenes in food samples by colony hybridization and rapid detection by PCR. Research in Microbiology 143, 271-280.
7. Bubert, A., Kohler, S. and Goebel, W. (1992) The homologous and heterologous regions within the iap gene allow genus-and species-specific identification of Listeria spp. by polymerase chain reaction. Applied and Environmental Microbiology 58, 2625-2632.
8. Bubert, A., Hein, I., Rauch, M., Lehner, A., Yoon, B., Goebel, W. and Wagner, M. (1999) Detection and differentiation of Listeria spp. by a single reaction based on multiplex PCR. Applied and Environmental Microbiology 65, 4688-4692.
9. Ebner, R. and Lengeler, J.W. (1988) DNA sequence of the gene scrA encoding the sucrose transport protein EnzymeII(Scr) of the phosphotransferase system from enteric bacteria: homology of the EnzymeII(Scr) and EnzymeII(Bgl) proteins. Molecular Microbiology 2, 9-17.
10. Encinas, J.P., Sanz, J.J., Garcia-Lopez, M.L. and Otero, A. (1999) Behaviour of Listeria spp. in naturally contaminated chorizo (Spanish fermented sausage). International Journal of Food Microbiology 46, 167-171.
11. Furrer, B., Candrian, U., Hoefelein, C. and Luethy, J. (1991) Detection and identification of Listeria monocytogenes in cooked sausage products and in milk by in vitro amplification of haemolysin gene fragments. Journal of Applied Bacteriology 70, 372-379.
12. Gilot, P. and Content, J. (2002) Specific identification of Listeria welshimeri and Listeria monocytogenes by PCR assays targeting a gene encoding a fibronectin-binding protein. Journal of Clinical Microbiology 40, 698-703.
13. Graham, T., Golsteyn-Thomas, E.J., Gannon, V.P. and Thomas, J.E. (1996) Genus-and species-specific detection of Listeria monocytogenes using polymerase chain reaction assays targeting the 16S/23S intergenic spacer region of the rRNA operon. Canadian Journal of Microbiology 42, 1155-1162.
14. Harvey, J. and Gilmour, A. (1993) Occurrence and characteristics of Listeria in foods produced in Northern Ireland. International Journal of Food Microbiology 19, 193-205.
15. Liu, D. (1994) Development of gene probes of Dichelobacter nodosus for differentiating strains causing virulent, intermediate or benign ovine foot rot. British Veterinary Journal 150, 451-462.
16. Liu, D. and Yong, W.K. (1993) Dichelobacter nodosus: differentiation of virulent and benign strains by gene probe based dot blot hybridisation. Veterinary Microbiology 38, 71-79.
17. Liu, D., Ainsworth, A.J., Austin, F.W. and Lawrence, M.L. (2003) Identification of Listeria innocua by PCR targeting a putative transcriptional regulator gene. FEMS Microbiology Letters 223, 205-210.
18. Rossen, L., Holmstrom, K., Olsen, J.E. and Rasmussen, O.F. (1991) A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples. International Journal of Food Microbiology 14, 145-151.
19. Sallen, B., Rajoharison, A., Desvarenne, S., Quinn, F. and Mabilat, C. (1996) Comparative analysis of 16S and 23S rRNA sequences of Listeria species. International Journal of Systematic Bacteriology 46, 669-674.
20. Seeliger, H.P. and Jones, D. (1984) Genus Listeria Pirie 1940, 383. In Bergey's Manual of Systematic Bacteriology ed. Holt, J.G. pp. 1235-1245. Baltimore, MD: Williams & Wilkins.
21. Senczek, D., Stephan, R. and Untermann, F. (2000) Pulsed-field gel electrophoresis (PFGE) typing of Listeria strains isolated from a meat processing plant over a 2-year period. International Journal of Food Microbiology 62, 155-159.
22. Starbuck, M.A., Hill, P.J. and Stewart, G.S. (1992) Ultra sensitive detection of Listeria monocytogenes in milk by the polymerase chain reaction (PCR). Letters in Applied Microbiology 15, 248-252.
23. Thomas, E.J., King, R.K., Burchak, J. and Gannon, V.P. (1991) Sensitive and specific detection of Listeria monocytogenes in milk and ground beef with the polymerase chain reaction. Applied and Environmental Microbiology 57, 2576-2580.
24. Vazquez-Boland, J.A., Kuhn, M., Berche, P., Chakraborty, T., Dominguez-Bernal, G., Goebel, W., Gonzalez-Zorn, B., Wehland, J. et al. (2001) Listeria pathogenesis and molecular virulence determinants. Clinical Microbiology Reviews 14, 584-640.