Immunocytochemical Localization of Histatins in Human Salivary Glands

Journal of Histochemistry and Cytochemistry

Volumn 52, Issue 3, 2004, Pages 361-370

  Ahmad, Mansur ^a   Piludu, Marco ^b   Oppenheim, Frank G ^c   Helmerhorst, Eva J ^c   Hand, Arthur R ^d,e  

^a UNIVERSITY OF MINNESOTA   (United States)

^b UNIVERSITY OF CAGLIARI   (Italy)

^c BOSTON UNIVERSITY   (United States)

^d UNIVERSITY OF CONNECTICUT   (United States)

^e UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

Author keywords

Antimicrobial proteins; Electron microscopy; Intercalated ducts; Parotid gland; Secretory granules; Serous cells; Sublingual gland; Submandibular gland

Indexed keywords

ANTIBODY; HISTATIN; HISTATIN ANTIBODY; HISTIDINE RICH PROTEINS; HISTIDINE-RICH PROTEINS; PROTEIN; SALIVA PROTEIN; UNCLASSIFIED DRUG;

ACINAR CELL; ADULT; ARTICLE; CELL GRANULE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ELECTRON MICROSCOPY; FEMALE; GOLGI COMPLEX; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOGOLD STAINING; IMMUNOHISTOCHEMISTRY; INTERCALATION COMPLEX; MALE; METABOLISM; METHODOLOGY; MICROSCOPY; MUCOUS CELL; PAROTID GLAND; PRIORITY JOURNAL; PROTEIN LOCALIZATION; ROUGH ENDOPLASMIC RETICULUM; SALIVA; SALIVARY GLAND; SECRETORY GRANULE; SILVER STAINING; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND; TISSUE SECTION; ULTRASTRUCTURE;

EID: 1242273841     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540405200307     Document Type: Article

References (48)

1. Atkinson JC, Yeh C-K, Oppenheim FG, Bermudez D, Baum BJ, Fox PC (1990) Elevation of salivary antimicrobial proteins following HIV-1 infection. J AIDS 3:41-48
2. Azen EA, Hellekant G, Sabatini LM, Warner TF (1990) mRNAs for PRPs, statherin, and histatins in von Ebner's gland tissues. J Dent Res 69:1724-1730
3. Baev D, Li XS, Dong J, Keng P, Edgerton M (2002) Human salivary histatin 5 causes disordered volume regulation and cell cycle arrest in Candida albicans. Infect Immun 70:4777-4784
4. Bercier JG, Al-Hashimi I, Haghighat N, Rees TD, Oppenheim FG (1999) Salivary histatins in patients with recurrent oral candidiasis. J Oral Pathol Med 28:26-29
5. Denny PC, Ball WD, Redman RS (1997) Salivary glands: a paradigm for diversity of gland development. Crit Rev Oral Biol Med 8:51-75
6. Fox PC, Heft MW, Herrera M, Bowers MR, Mandel ID, Baum BJ (1987) Secretion of antimicrobial proteins from the parotid glands of different aged healthy persons. J Gerontol 42:466-469
7. Gusman H, Travis J, Helmerhorst EJ, Potempa J, Troxler RF, Oppenheim FG (2001) Salivary histatin 5 is an inhibitor of both host and bacterial enzymes implicated in periodontal disease. Infect Immun 69:1402-1408
8. Hand AR (1995) Electron microscopy. In Glasel JA, Deutscher M, eds. Introduction to Biophysical Methods for Protein and Nucleic Acid Research. New York, Academic Press, 205-260
9. Hand AR, Ho B (1985) Mitosis and hypertrophy of intercalated duct cells and endothelial cells in the isoproterenol-treated rat parotid gland. J Dent Res 64:1031-1038
10. Helmerhorst EJ, Breeuwer P, van't Hof W, Walgreen-Weterings E, Oomen LC, Veerman EC, Amerongen AV, et al. (1999) The cellular target of histatin 5 on Candida albicans is the energized mitochondrion. J Biol Chem 274:7286-7291
11. Helmerhorst EJ, Troxler RF, Oppenheim FG (2001) The human salivary peptide histatin 5 exerts its antifungal activity through the formation of reactive oxygen species. Proc Natl Acad Sci USA 98:14637-14642
12. Ichikawa M, Ichikawa A (1987) The fine structure of sublingual gland acinar cells of the Mongolian gerbil, Meriones unguiculatus, processed by rapid freezing followed by freeze-substitution fixation. Cell Tissue Res 250:305-314
13. Ichikawa A, Ichikawa M, Hirokawa N (1980) The ultrastructure of rapid-frozen, substitution fixed parotid gland acinar cells of the Mongolian gerbil (Meriones meridianus). Am J Anat 157:107-110
14. Ionrcheva I, Oppenheim FG, Troxler RF (1997) Human salivary mucin MG1 selectively forms heterotypic complexes with amylase, proline-rich proteins, statherin, and histatins. J Dent Res 76:734-743
15. Jensen JL, Xu T, Lamkin MS, Brodin P, Aars H, Berg T, Oppenheim FG (1994) Physiological regulation of the secretion of histatins and statherins in human parotid saliva. J Dent Res 73:1811-1817
16. Johnson DA, Yeh CK, Dodds MW (2000) Effect of donor age on the concentrations of histatins in human parotid and submandibular/sublingual saliva. Arch Oral Biol 45:731-740
17. Kimura I, Sasamura T, Tsuneki H, Iwai M, Furuta I, Sasamoto H, Furusawa Y (1998) Immunocytochemical localization of salivary peptide P-C in human submandibular gland. Biol Pharm Bull 21:506-512
18. Korsrud FR, Brandtzaeg P (1982) Characterization of epithelial elements in human major salivary glands by functional markers: localization of amylase, lactoferrin, lysozyme, secretory component, and secretory immunoglobulins by paired immunofluorescence staining. J Histochem Cytochem 30:657-666
19. Koshlukova SE, Lloyd TL, Araujo MW, Edgerton M (1999) Salivary histatin 5 induces non-lytic release of ATP from Candida albicans leading to cell death. J Biol Chem 274:18872-18879
20. Lal K, Pollock JJ, Santarpia RP III, Heller HM, Kaufman HW, Fuhrer J, Steigbigel RT (1992) Pilot study comparing the salivary cationic protein concentrations in healthy adults and AIDS patients: correlation with antifungal activity. J Acquir Immune Defic Syndr 5:904-914
21. Lantini MS, Valentino L, Riva A (1988) A granular cell in the proximal intercalated duct of human parotid and submandibular glands. J Submicrosc Cytol Pathol 20:147-152
22. Lin AL, Johnson DA, Patterson TF, Wu Y, Lu DL, Shi Q, Yeh C-K (2001) Salivary anticandidal activity and saliva composition in an HIV-infected cohort. Oral Microbiol Immunol 16:270-278
23. Machino M, Morioka H, Tachibana M (1986) Amylase and lysozyme differentiate their localization within the serous secretory granule of the human salivary gland. Acta Histochem Cytochem 19:329-332
24. Mandel ID, Barr CE, Turgeon L (1992) Longitudinal study of parotid saliva in HIV-1 infection. J Oral Pathol Med 21:209-213
25. Miyazaki T, Inoue Y, Takano K (2001) Seromucous cells in human sublingual glands: examination by immunocytochemistry of lysozyme. Arch Histol Cytol 64:305-312
26. Oppenheim FG, Xu T, McMillan FM, Levitz SM, Diamond RD, Offner GD, Troxler RF (1988) Histatins, a novel family of histidine-rich proteins in human parotid secretion. Isolation, characterization, primary structure, and fungistatic effects on Candida albicans. J Biol Chem 263:7472-7477
27. Oppenheim FG, Yang Y-C, Diamond RD, Hylsop D, Offner GD, Troxler RF (1986) The primary structure and functional characterization of the neutral histidine-rich polypeptide from human parotid secretion. J Biol Chem 261:1177-1182
28. Piludu M, Mednieks MI, Hand AR (2002) Cyclic AMP-receptor proteins in human salivary glands. Eur J Morphol 40:219-225
29. Piludu M, Rayment SA, Liu B, Offner GD, Oppenheim FG, Troxler RF, Hand AR (2003) Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands. J Histochem Cytochem 51:69-79
30. Perinpanayagam HER, Van Wuyckhuyse BC, Ji ZS, Tabak LA (1995) Characterization of low-molecular weight peptides in human parotid saliva. J Dent Res 74:345-350
31. Prakobphol A, Xu F, Hoang VM, Larsson T, Bergstrom J, Johansson I, Frangsmyr L, et al. (2000) Salivary agglutinin, which binds Streptococcus mutans and Helicobacter pylori, is the lung scavenger receptor cysteine-rich protein gp-340. J Biol Chem 275:39860-39866
32. Redman RS, Field RB (1993) Chronology of peroxidase activity in the developing rat parotid gland. Anat Rec 235:611-621
33. Rijnkels M, Elnitski L, Miller W, Rosen JM (2003) Multispecies comparative analysis of a mammalian-specific genomic domain encoding secretory proteins. Genomics 82:417-432
34. Riva A, Motta G, Riva-Testa F (1974) Ultrastructural diversity in secretory granules of human major salivary glands. Am J Anat 139:293-298
35. Riva A, Tandler B, Testa Riva F (1988) Ultrastructural observations on human sublingual gland. Am J Anat 181:385-392
36. Sabatini LM, Azen EA (1989) Histatins, a family of salivary histidine-rich proteins, are encoded by at least two loci (HIS1 and HIS2). Biochem Biophys Res Commun 160:495-502
37. Sabatini LM, Ota T, Azen EA (1993) Nucleotide sequence analysis of the human salivary protein genes HIS1 and HIS2 and evolution of the STATH/HIS gene family. Mol Biol Evol 10:497-511
38. Schenkels LCPM, Veerman ECI, Nieuw Amerongen AV (1995) Biochemical composition of human saliva in relation to other mucosal fluids. J Dent Res 6:161-175
39. Sivakumar S, Mirels L, Miranda AJ, Hand AR (1998) Secretory protein expression patterns during rat parotid gland development. Anat Rec 252:485-497
40. Takano K, Bogert M, Malamud D, Lally E, Hand AR (1991) Differential distribution of salivary agglutinin and amylase in the Golgi apparatus and secretory granules of human salivary gland acinar cells. Anat Rec 230:307-318
41. Takano K, Malamud D, Bennick A, Oppenheim F, Hand AR (1993) Localization of salivary proteins in granules of human parotid and submandibular acinar cells. Crit Rev Oral Biol Med 4:399-405
42. Tandler B, Erlandson RA (1972) Ultrastructure of the human submaxillary gland. IV. Serous granules. Am J Anat 135:419-433
43. Tandler B, Phillips CJ (1993) Structure of serous cells in salivary glands. Microsc Res Tech 26:32-48
44. Troxler RF, Offner GD, Xu T, Vanderspek JC, Oppenheim FG (1990) Structural relationship between human salivary histatins. J Dent Res 69:2-6
45. Vugman I, Hand AR (1995) Quantitative immunocytochemical study of secretory protein expression in parotid glands of rats chronically treated with isoproterenol. Microsc Res Tech 31:106-117
46. Wu AJ, Atkinson JC, Fox PC, Baum BJ, Ship JA (1993) Cross-sectional and longitudinal analyses of stimulated parotid salivary constituents in healthy, different-aged subjects. J Gerontol 48:M219-224
47. Xu Y, Ambudkar I, Yamagishi H, Swaim W, Walsh TJ, O'Connell BC (1999) Histatin 3-mediated killing of Candida albicans: effect of extracellular salt concentration on binding and internalization. Antimicrob Agents Chemother 43:2256-2262
48. Xu T, Telser E, Troxler RF, Oppenheim FG (1990) Primary structure and anticandidal activity of the major histatin from parotid secretion of the subhuman primate, Macaca fascicularis. J Dent Res 69:1717-1723