Human salivary mucin MG1 selectively forms heterotypic complexes with amylase, proline-rich proteins, statherin, and histatins

Journal of Dental Research

Volumn 76, Issue 3, 1997, Pages 734-743

  Iontcheva, I ^a   Oppenheim, F G ^a,b   Troxler, R F ^a,b  

^a BOSTON UNIVERSITY MEDICAL CENTER   (United States)

^b BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Covalent non covalent complex; ELISA; Gel filtration; Submandibular sublingual secretion; Western blotting

Indexed keywords

AMINO ACID; AMYLASE; HISTIDINE RICH PROTEINS; HISTIDINE-RICH PROTEINS; MUCIN; PROLINE; PROTEIN; SALIVA PROTEIN; STATHERIN;

ARTICLE; CHEMISTRY; DENTAL PELLICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DENATURATION; SALIVA; WESTERN BLOTTING;

AMINO ACIDS; AMYLASES; BLOTTING, WESTERN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DENTAL PELLICLE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; MOLECULAR WEIGHT; MUCINS; PROLINE; PROTEIN DENATURATION; PROTEINS; SALIVA; SALIVARY PROTEINS;

EID: 0031089153     PISSN: 00220345     EISSN: None     Source Type: Journal    
DOI: 10.1177/00220345970760030501     Document Type: Article

References (43)

1. Aguirre A, Testa-Weintraub LA, Banderas AJ, Haraszthy GG, Reddy MS, Levine MJ (1993). Sialochemistry: A diagnostic tool? Crit Rev Oral Biol Med 4:343-350.
2. Al-Hashimi I, Levine MJ (1989). Characterization of in vivo salivary-derived enamel pellicle. Arch Oral Biol 34:289-295.
3. Belford HS, Triffleman EG, Offner GD, Troxler RF, Oppenheim FG (1984). Biosynthesis of salivary proteins in the parotid gland of the subhuman primate, Macaca fascicularis. Cell-free translation of the mRNA for a proline-rich glycoprotein and partial amino acid sequence and processing of its signal peptide. J Biol Chem 259:3977-3984.
4. Bella A Jr, Kim YS (1973). Iron binding of gastric mucin. Biochim Biophys Acta 304:580-585.
5. Biesbrock AR, Reddy MS, Levine MJ (1991). Interaction of a salivary mucin-secretory immunoglobulin A complex with mucosal pathogens. Infect Immun 59:3492-3497.
6. Bradway SD, Bergey EJ, Scannapieco FA, Ramasubbu N, Zawacki S, Levine MJ (1992). Formation of salivary-mucosal pellicle: the role of transglutaminase. Biochem J 284:557-564.
7. Cohen RE, Levine MJ (1989). Salivary glycoproteins. In: Human saliva: Clinical chemistry and microbiology. Tenuovo J, editor. Boca Raton, FL: CRC Press, pp. 101-130.
8. Douglas CW (1990). Characterization of the alpha-amylase receptor of Streptococcus gordonii NCTC 7868. J Dent Res 69:1746-1752.
9. Douglas WH, Reeh ES, Ramasubbu N, Raj PA, Bhandary KK, Levine MJ (1991). Statherin: a major boundary lubricant in human saliva. Biochem Biophys Res Commun 180:91-97.
10. Forstner JF, Forstner GG (1975). Calcium binding to intestinal goblet cell mucin. Biochem Biophys Acta 386:283-292.
11. Gupta R, Jentoft N (1989). Subunit structure of porcine submaxillary mucin. Biochemistry 28:6114-6121.
12. Hatton MN, Loomis RE, Levine MJ, Tabak LA (1985). Masticatory lubrication. The role of carbohydrate in the lubricating property of a salivary glycoprotein-albumin complex. Biochem J 230:817-820.
13. Hay DI, Bennick A, Schlesinger DH, Minaguchi K, Madapallimattam G, Schluckebier SK (1988). The primary structures of six human salivary acidic proline-rich proteins (PRP-1, PRP-2, PRP-3, PRP-4, PIF-s and PIF-f). Biochem J 255:15-21.
14. Jensen JL, Lamkin MS, Oppenheim FG (1992). Adsorption of human salivary proteins to hydroxyapatite: A comparison between whole saliva and glandular salivary secretions. J Dent Res 71:1569-1576.
15. Jensen JL, Xu T, Lamkin MS, Brodin P, Aars HJ, Berg T, et al. (1994). Physiological regulation of the secretion of histatins and statherins in human parotid saliva. J Dent Res 73:1811-1817.
16. Kawagishi S, Fahim REF, Wong KH, Bennick A (1990). Purification and characterization of subunits of a high molecular weight human salivary mucin. Arch Oral Biol 35:265-272.
17. Kilian M, Nyvad B (1990). Ability to bind salivary alpha amylase discriminates certain viridans streptococcal species. J Clin Microbiol 28:2576-2577.
18. Kousvelari EE, Baratz RS, Burkei B, Oppenheim FG (1980). Immunochemical identification and determination of proline-rich proteins in salivary secretions, enamel pellicle and glandular tissue specimens. J Dent Res 59:1430-1438.
19. Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
20. Lamkin MS, Migliari D, Troxler RF, Oppenheim FG (1995). Novel post-translational processing of proteins in whole saliva (abstract). J Dent Res 74:85.
21. Levine MJ (1993). Development of artificial salivas. Crit Rev Oral Biol Med 4:279-286.
22. Levine MJ, Reddy MS, Tabak LA, Loomis RE, Bergey EJ, Jones PC, et al. (1987). Structural aspects of salivary glycoproteins. J Dent Res 66:436-441.
23. Loomis RE, Prakobphol A, Levine MJ, Reddy MS, Jones PC (1987). Biochemical and biophysical comparison of two mucins from human submandibular-sublingual saliva. Arch Biochem Biophys 258:452-464.
24. Luyten FP, Cunningham NS, Ma S, Muthukumaran N, Hammonds RG, Nevins WB, et al. (1989). Purification and partial amino acid sequence of osteogenin, a protein initiating bone differentiation. J Biol Chem 264:13377-13380.
25. Mantle M, Allen A (1978). A colorimetric assay for glycoproteins based on the periodic/Schiff stain. Biochem Soc Trans 6:607-609.
26. Mayo GW, Carlson DM (1974). Isolation and properties of four alpha-amylase isoenzymes from human submandibular saliva. Arch Biochem Biophys 163:498-506.
27. Oppenheim FG, Xu T, McMillian FM, Levitz SM, Diamond RD, Offner GD, et al. (1988). Histatins, a novel family of histidine-rich proteins in human parotid secretion. Isolation, characterization, primary structure, and fungistatic effects on Candida albicans. J Biol Chem 263:7472-7477.
28. Prakobphol A, Levine MJ, Tabak LA, Reddy MS (1982). Purification of a low molecular weight mucin-type glycoprotein from human submandibular-sublingual saliva. Carbohydr Res 108:111-122.
29. Ramasubbu N, Reddy MS, Bergey EJ, Haraszthy GG, Soni SD, Levine MJ (1991). Large-scale purification and characterization of the major phosphoproteins and mucins of human submandibular-sublingual saliva. Biochem J 280:341-352.
30. Scannapieco FA (1993). Saliva-bacterium interactions in oral microbial ecology. Crit Rev Oral Biol Med 5:203-248.
31. Scannapieco FA, Levine MJ (1993). Salivary mucins and dental plaque formation. In: Cariology for the nineties. Bowen WH, Tabak LA, editors. Rochester, NY: University of Rochester Press, pp. 87-105.
32. Scannapieco FA, Bergey EJ, Reddy MS, Levine MJ (1989). Characterization of salivary alpha amylase binding to Streptococcus sanguis. Infect Immun 57:2853-2863.
33. Scannapieco FA, Torres G, Levine MJ (1993). Salivary α-amylase: Role in dental plaque and caries formation. Crit Rev Oral Biol Med 4:301-307.
34. Schlesinger DH, Hay DI (1977). Complete covalent structure of statherin, a tyrosine-rich acidic peptide which inhibits calcium phosphate precipitation from human parotid saliva. J Biol Chem 252:1689-1695.
35. Seagrest JP, Jackson RL (1972). Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis in sodium dodecylsulfate. Meth Enzymol 28:54-63.
36. Shomers JP, Tabak LA, Levine MJ, Mandel ID, Ellison SA (1982). The isolation of a family of cysteine-containing phosphoproteins from human submandibular-sublingual saliva. J Dent Res 61:973-977.
37. Snary D, Allen A, Pain RH (1974). Conformation changes in gastric mucoproteins induced by caesium chloride and guanidinium chloride. Biochem J 141:641-646.
38. Strous GJ, Dekker J (1992). Mucin-type glycoproteins. Crit Rev Biochem Molec Biol 27:57-92.
39. Tabak LA (1995). In defense of the oral cavity: Structure, biosynthesis and function of salivary mucins. Ann Rev Physiol 57:547-564.
40. Towbin H, Staehelin T, Gordon G (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci (USA) 76:4350-4354.
41. Troxler RF, Offner GD, Zhang F, Iontcheva I, Oppenheim FG (1995). Molecular cloning of a novel high molecular weight mucin (MG1) from human sublingual gland. Biochem Biophys Res Commun 217:1112-1119.
42. Troxler RF, Offner GD, Zhang R, Iontcheva I, Oppenheim FG (1996). Molecular cloning of a human sublingual gland mucin (MG1) (abstract). J Dent Res 75:357.
43. Wu AM, Csako G, Herp A (1994). Structure, biosynthesis and function of salivary mucins. Molec Cell Biochem 137:39-55.