A practical method for enumerating cysts of ciliates in natural marine sediments

Aquatic Microbial Ecology

Volumn 37, Issue 3, 2004, Pages 305-310

  Ichinomiya, Mutsuo ^a   Nakamachi, Miwa ^a   Taniguchi, Akira ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

Cysts; Density gradient centrifugation; Enumeration; Glutaraldehyde; Natural sediments; Planktonic ciliates

Indexed keywords

CILIOPHORA;

EID: 12344313426     PISSN: 09483055     EISSN: None     Source Type: Journal    
DOI: 10.3354/ame037305     Document Type: Article

References (24)

1. Blanco J (1986) Separacion de quistes de dinoflagelados en gradiente de densidad. Bol Inst Esp Oceanogr 3:81-84
2. Bolch CJS (1997) The use of sodium polytugstate for the separation and concentration of living dinoflagellate cysts from marine sediments. Phycologia 36:472-478
3. De Jonge VN (1979) Quantitative separation of benthic diatoms from sediments using density gradient centrifugation in the colloidal silica Ludox-TM. Mar Biol 51:267-278
4. Epstein SS (1995) Simultaneous enumeration of protozoa and micrometazoa from marine sandy sediments. Aquat Microb Ecol 9:219-227
5. Haraguchi CM, Yokota S (2002) Immunofluorescence technique for 100-nm-thick semithin sections of Epon-embedded tissues. Histochem Cell Biol 117:81-85
6. Imai I, Itoh K, Anraku M (1984) Extinction dilution method for enumeration of dormant cells of red tide organisms in marine sediments. Bull Plankton Soc Jpn 31:123-124
7. Johnson MD, Rome M, Stoecker DK (2003) Microzooplankton grazing on Prorocentrum minimum and Karlodinium micrum in Chesapeake Bay. Limnol Oceanogr 48:238-248
8. Kamiyama T (1996) Determination of the abundance of viable tintinnid cysts in marine sediments in Hiroshima Bay, the Seto Inland Sea of Japan, using a modified MPN method. J Plankton Res 18:1253-1259
9. Kamiyama T, Anzai Y (1990) Excystment of tintinnid ciliates from marine sediment. Bull Plankton Soc Jpn 36:138-139
10. Kim YO (1995) Life cycle strategies of choreorich ciliate plankton in Onagawa Bay. PhD thesis, Tohoku University, Sendai
11. Kim YO, Taniguchi A (1995) Excystment of the oligotrich ciliate Strombidium conicum. Aquat Microb Ecol 9:149-156
12. Kim YO, Taniguchi A (1997) Seasonal variation of excystment pattern of the planktonic oligotrich ciliate Strombidium conicum. Mar Biol 128:207-212
13. Kim YO, Suzuki T, Taniguchi A (2002) A new species in the Genus Cyrtostrombidium (Cliliophora, Oligotrichia, Oligotrichida): its morphology, seasonal cycle and resting stage. J Eukaryot Microbiol 49:338-343
14. Matsuoka K, Fukuyo Y, Anderson DM (1989) Method for modern dinoflagellate cyst studies. In: Okaichi T, Anderson DM, Nemoto T (eds) Red tides; biology, environmental science, and toxicology. Elsevier, New York, p 461-479
15. Müller H (1996) Encystment of the fresh water ciliate Pelagostrombidium fallax (Ciliophora, Oligotrichida) in laboratory culture. Aquat Microb Ecol 11:289-295
16. Müller H, Wünsch C (1999) Seasonal dynamics of cyst formation of pelagic strombidiid ciliates in a deep pre-alpine lake. Aquat Microb Ecol 17:37-47
17. Müller H, Stadler P, Welsse T (2002) Seasonal dynamics of cyst formation of strombidiid ciliates in alpine Lake Mondsee, Austria. Aquat Microb Ecol 20:181-188
18. Paranjape M (1980) Occurrence and significance of resting cysts in a hyaline tintinnid, Helicostomella subulata (Ehre) Jörgensen. J Exp Mar Biol Ecol 48:23-33
19. Reid PC (1987) Mass excystment of a planktonic oligotrich ciliate. Mar Biol 95:221-230
20. Reid PC, John AW (1978) Tintinnid cysts. J Mar Biol Assoc UK 58:551-557
21. Reid PC, John AW (1983) Resting cysts in the ciliate class Polymenophores: phylogenetic implications. J Protozool 30:710-713
22. Schwinghamer P, Anderson DM, Kulis DM (1991) Separation and concentration of living dinoflagellate resting cysts from marine sediments via density-gradient centrifugation. Limnol Oceanogr 36:588-592
23. Weber K, Rathke PC, Osborn M (1978) Cytoplasmic microtubular images in glutaraldehyde-fixed tissue culture cells by electron microscopy and by immunofluorescence microscopy. Proc Natl Acad Sci USA 75:1820-1824
24. Yamaguchi M, Itakura S, Imai I, Ishida Y (1995) A rapid and precise technique for enumeration of resting cysts of Alexandrium spp. (Dinophyceae) in natural sediments. Phycologia 34:207-214