Immunofluorescence technique for 100-nm-thick semithin sections of Epon-embedded tissues

Histochemistry and Cell Biology

Volumn 117, Issue 1, 2002, Pages 81-85

  Haraguchi, Celina M ^a   Yokota, Sadaki ^a  

^a UNIVERSITY OF YAMANASHI   (Japan)

Author keywords

Deplasticization; Immunofluorescence staining; Semithin Epon sections

Indexed keywords

ACETYL COENZYME A ACYLTRANSFERASE; ANTIBODY; CATALASE; CATHEPSIN D; CYTOSOL AMINOPEPTIDASE; DYE; EPOXY RESIN; GLASS; GLUTARALDEHYDE; LIQUEFIED PETROLEUM GAS; SODIUM BOROHYDRIDE; SODIUM DERIVATIVE; TRYPSIN;

ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; CELL MEMBRANE; CHEMICAL LABELING; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; EMBEDDING; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; KIDNEY; LIVER; LYSOSOME; LYSOSOME MEMBRANE; MALE; METHODOLOGY; MITOCHONDRION; NONHUMAN; PEROXISOME; PRIORITY JOURNAL; RAT; THICKNESS; TISSUE FIXATION; TISSUE SECTION; TISSUE SLICE;

ACETYL-COA C-ACYLTRANSFERASE; ANIMALS; CATALASE; CATHEPSIN D; EPOXY RESINS; FLUORESCENT ANTIBODY TECHNIQUE; KIDNEY; LEUCYL AMINOPEPTIDASE; LIVER; LYSOSOMES; MICROSCOPY, FLUORESCENCE; MICROTOMY; RATS; TISSUE EMBEDDING;

ALEXA; ANIMALIA;

EID: 0036254718     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00418-001-0363-1     Document Type: Article

References (12)

1. Correlation of some mechanical properties of embedding resins with their behavior in microtomy
2. Autofluorescence and induced fluorescence in Epon embedded tissue sections
3. Immunological properties of an antibody containing a fluorescent group
4. Biochemical analysis of the movement of a major lysosomal membrane glycoprotein in the endocytic membrane system
5. Light microscopic immunocytochemical demonstration of peroxisomal enzymes in Epon sections
6. Individual microtubules viewed by immunofluorescence and electron microscopy in the same PtK2 cell
7. Immunocytochemical localization of D-amino acid oxidase in the central clear matrix of rat kidney peroxisomes
8. Quantitative immunoelectron microscopy for proteins contained in the cell organelles: Evaluation with a model system
9. Localization of cathepsin D in rat liver. Immunocytochemical study using post-embedding immunoenzyme and protein A-gold techniques
10. Immunoelectron microscopy of peroxisomal enzymes: Their substructural association and compartmentalization in rat kidney peroxisomes
11. Formation of autophagosomes during degradation of excess peroxisomes induced by di-(2-ethylhexyl)phthalate treatment. III. Fusion of early autophagosomes with lysosomal compartments