Comparison of Two Glucoamylases Produced by Aspergillus oryzae in Solid-State Culture (Koji) and in Submerged Culture

Journal of Fermentation and Bioengineering

Volumn 84, Issue 6, 1997, Pages 532-537

  Hata, Yoji ^a   Ishida, Hiroki ^a   Kojima, Yasuhiro ^a   Ichikawa, Eiji ^a   Kawato, Akitsugu ^a   Suginami, Koji ^a   Imayasu, Satoshi ^a  

^a Gekkeikan Sake Company Ltd   (Japan)

Author keywords

Aspergillus oryzae; GlaA; Glucoamylase; Solid state culture

Indexed keywords

AMINO ACIDS; BIOCONVERSION; BIOSYNTHESIS; CLONING; ENZYMES; STARCH; THERMODYNAMIC STABILITY;

GLUCOAMYLASE; POLYPEPTIDES; PROTEOLYTIC PROCESSING; SOLID STATE CELL CULTURE; SUBMERGED CELL CULTURE;

CELL CULTURE;

EID: 0031342633     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0922-338X(97)81907-1     Document Type: Article

References (24)

1. Nunokawa, Y.: Enzymes in saké fermentation. J. Jpn. Soc. Starch Sei., 33, 95-103 (1986).
2. Morita, Y., Shimizu, K., Ohga, M., and Korenaga, T.: Studies on amylases of Aspergillus oryzae cultured on rice. I. Isolation and purification of glucoamylase. Agric. Biol. Chem., 30, 114-121 (1966).
3. Ohga, M., Shimizu, K. and Morita, Y.: Studies on amylases of Aspergillus oryzae cultured on rice. II. Some properties of glucoamylase. Agric. Biol. Chem., 30, 967-972 (1966).
4. Miah, M. N. N. and Ueda, S.: Multiplicity of glucoamylase of Aspergillus oryzae. I. Separation and purification of three forms of glucoamylase. Die Stearke, 29, 191-196 (1977).
5. Miah, M. N. N., and Ueda, S.: Multiplicity of glcucoamylase of Aspergillus oryzae. II. Enzymatic and physiochemical properties of three forms of glucoamylase. Die Stearke, 29, 235239 (1977).
6. Saha, B.C., Mitsue, T., and Ueda, S.: Glucoamylase produced by submerged culture of Aspergillus oryzae. Die Stearke, 31, 307-314 (1979).
7. Mitsue, T., Saha, B. C., and Ueda, S.: Glucoamylase of Aspergillus oryzae cultured of steamed rice. J. Appl. Biochem., 1, 410-422 (1979).
8. Ono, K., Shigeta, S., and Oka, S.: Effective purification of glucoamylase in koji, a solid culture of Aspergillus oryzae on steamed rice, by affinity chromatography using an immobilized acarbose (BAY g-5421). Agric. Biol. Chem., 52, 1707-1714 (1988).
9. Hata, Y., Kitamoto, K., Gomi, K., Kumagai, C., Tamura, G. and Hara, S.: The glucoamylase cDNA from Aspergillus oryzae; its cloning, nucleotide sequence, and expression in Saccharomyces cerevisiae. Agric. Biol. Chem., 55, 941-949 (1991).
10. Hata, Y., Tsuchiya, K., Kitamoto, K., Gomi, K., Kumagai, C., Tamura, G., and Hara, S.: Nucleotide sequence and expression of the glucoamylase-encoding gene (glaA) from Aspergillus oryzae. Gene., 108, 145-150 (1992).
11. Nagashima, T., Yamamoto, Y., Kitamoto, K., and Kumagai, C.: Function role of A. oryzae glucoamylase C-terminal domain investigated using its cDNA. J. Ferment. Bioeng., 80, 280-282 (1995).
12. Svensson, B., Larsen, K., and Gimnarsson, A.: Characterization of a glucoamylase G2 from Aspergillus niger. Eur. J. Biochem., 154, 497-502 (1986).
13. Hayasida, S., Nakahara, K., Kanlayakrit, W., Hara, T., and Teramoto, Y.: Characteristic and function of raw-starch-amnity site on Aspergillus awamori var. kawachi glucoamylase I molecule. Agric. Biol. Chem., 53, 143-149 (1989).
14. Iwano, K., Fukuda, K., Shiinoki, S., and Shimada, T.: The properties of various enzymes of shochu koji (Aspergillus kawachii). J. Brew. Soc. Jpn., 81, 490-494 (1986).
15. Bradford, J. F.: A rapid and sensitive method for the quantilation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 1513-1523 (1979).
16. Crestified, A. M., Moore, S. and Stein, W. H.: The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated protein. J. Biol. Chem., 238, 622-627 (1963).
17. Tulsiani, D. R. P. and Tonster, O.: Swainsonine cause the production of hybrid glycoproteins by human skin fibroblasts and rat liver golgi preparation. J. Biol. Chem., 258, 7578-7585 (1983).
18. Unkles, S.E., Campbel, E.I., de Ruiter-Jacobs, Y.M.J.T., Broekhuijsen, M., Marco, J. A., Carrez, D., Contreras, Ä., van den Hondel, C. A. M. J.J., and Kingdom, J. R.: The development of a homologous transformation system for Aspergillus oryzae based on the nitrate assimilation pathway: a convenient and general selection system for filamentous fungal transformation. Mol. Gen. Genet., 218, 99-104 (1989).
19. limura, Y., Gomi, K., Uzu, H., and Hara, S.: Transformation of Aspergillus oryzae through plasmid-mediated complementation of the methionine-auxotrophic mutation. Agric. Biol. Chem., 51, 323-328 (1987).
20. Boel, E., Hjort, L, Hansen, M. T., Svensson, B., Norris, F., Norris, K. E., and Fill, N. P.: Glucoamylases Gl and G2 from Aspergillus niger are synthesized from two different but closely related mRNAs. EMBO J., 3, 1097-1102 (1984).
21. Boel, E., Hansen, M. T., Hjort, I., Hoegh, I., and Fill, N. P.: Two different types of intervening sequences in the glucoamylase gene from Aspergillus niger. EMBO J., 3, 1581-1585 (1984).
22. Marshall, R. D.: Glycoprotein. Annu. Rev. Biochem., 41, 673-681 (1972).
23. Narahara, H., Koyama, Y., Yoshida, T., Pichangknra, S., Ueda, R., and Taguchi, H.: Growth and enzyme production in a solid-state culture of Aspergillus oryzae, J. Ferment. Technol., 60, 311-319(1982).
24. Laemmli, U. K.: Cleavage of structure proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).