Uridine Recognition Motifs of Human Equilibrative Nucleoside Transporters 1 and 2 Produced in Saccharomyces cerevisiae

Nucleosides, Nucleotides and Nucleic Acids

Volumn 23, Issue 1-2, 2004, Pages 361-373

  Vickers, Mark F ^a,b   Zhang, Jing ^a,b   Visser, Frank ^a,b   Tackaberry, Tracey ^a,b   Robins, Morris J ^c   Nielsen, Lars P C ^c   Nowak, Ireneusz ^c   Baldwin, Stephen A ^d   Young, James D ^a   Cass, Carol E ^a,b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b CROSS CANCER INSTITUTE   (Canada)

^c Brigham Young University   (United States)

^d UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

hENT1; hENT2; Saccharomyces cerevisiae; Uridine recognition motifs

Indexed keywords

METHYL GROUP; NUCLEOSIDE TRANSPORTER; NUCLEOSIDE TRANSPORTER 1; NUCLEOSIDE TRANSPORTER 2; RECOMBINANT PROTEIN; SUGAR; UNCLASSIFIED DRUG; URIDINE; URIDINE DERIVATIVE;

ARTICLE; BINDING AFFINITY; HUMAN; NONHUMAN; NUCLEOSIDE TRANSPORT; NUCLEOTIDE SEQUENCE; PROTEIN BINDING; PROTEIN MODIFICATION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; URIDINE RECOGNITION MOTIF;

AMINO ACID MOTIFS; CLONING, MOLECULAR; EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1; EQUILIBRATIVE-NUCLEOSIDE TRANSPORTER 2; HUMANS; KINETICS; SACCHAROMYCES CEREVISIAE; THERMODYNAMICS; URIDINE;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 12144287868     PISSN: 15257770     EISSN: None     Source Type: Journal    
DOI: 10.1081/NCN-120028333     Document Type: Article

References (30)

1. Vickers, M.F.; Young, J.D.; Baldwin, S.A.; Mackey, J.R.; Cass, C.E. Nucleoside transporter proteins: emerging targets for drug discovery. Emerg. Ther. Targets 2000, 4 (4), 515-539.
2. Mackey, J.R.; Baldwin, S.A.; Young, J.D.; Cass, C.E. Nucleoside transport and its significance for anticancer drug resistance. Drug Resist. Updat. 1998, 1, 310-324.
3. +-dependent nucleoside transporters. Pharm. Res. 1997, 14 (11), 1524-1532.
4. Burnstock, G. Potential therapeutic targets in the rapidly expanding field of purinergic signalling. Clin. Med. 2002, 2 (1), 45-53.
5. Latini, S.; Pedata, F. Adenosine in the central nervous system: release mechanisms and extracellular concentrations. J. Neurochem. 2001, 79 (3), 463-484.
6. Ward, J.L.; Sherali, A.; Mo, Z.P.; Tse, C.M. Kinetic and pharmacological properties of cloned human equilibrative nucleoside transporters, ENT1 and ENT2, stably expressed in nucleoside transporter-deficient PK15 cells. Ent2 exhibits a low affinity for guanosine and cytidine but a high affinity for inosine. J. Biol. Chem. 2000, 275 (12), 8375-8381.
7. Griffiths, M.; Beaumont, N.; Yao, S.Y.; Sundaram, M.; Boumah, C.E.; Davies, A.; Kwong, F.Y.; Coe, I.; Cass, C.E.; Young, J.D.; Baldwin, S.A. Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs. Nat. Med. 1997, 3 (1), 89-93.
8. Griffiths, M.; Yao, S.Y.; Abidi, F.; Phillips, S.E.; Cass, C.E.; Young, J.D.; Baldwin, S.A. Molecular cloning and characterization of a nitrobenzylthioinosine-insensitive (ei) equilibrative nucleoside transporter from human placenta. Biochem. J. 1997, 328 (Pt 3), 739-743.
9. Yao, S.Y.; Ng, A.M.; Vickers, M.F.; Sundaram, M.; Cass, C.E.; Baldwin, S.A.; Young, J.D. Functional and molecular characterization of nucleobase transport by recombinant human and rat equilibrative nucleoside transporters 1 and 2. Chimeric constructs reveal a role for the ENT2 helix 5-6 region in nucleobase translocation. J. Biol. Chem. 2002, 277 (28), 24938-24948.
10. Visser, F.; Vickers, M.F.; Ng, A.M.; Baldwin, S.A.; Young, J.D.; Cass, C.E. Mutation of residue 33 of human equilibrative nucleoside transporters 1 and 2 (hENT1 and hENT2) alters sensitivity to inhibition of transport by dilazep and dipyridamole. J. Biol. Chem. 2002, 277 (1), 395-401.
11. Sundaram, M.; Yao, S.Y.M.; Ng, A.M.L.; Griffiths, M.; Cass, C.E.; Baldwin, S.A.; Young, J.D. Chimeric constructs between human and rat equilibrative nucleoside transporters (hENT1 and rENT1) reveal hENT1 structural domains interacting with coronary vasoactive drugs. J. Biol. Chem. 1998, 273 (34), 21519-21525.
12. Yao, S.Y.; Ng, A.M.; Ritzel, M.W.; Gati, W.P.; Cass, C.E.; Young, J.D. Transport of adenosine by recombinant purine-and pyrimidine-selective sodium/nucleoside cotransporters from rat jejunum expressed in Xenopus laevis oocytes. Mol. Pharmacol. 1996, 50 (6), 1529-1535.
13. +-nucleoside cotransporter. J. Biol. Chem. 1995, 270 (23), 13596-13599.
14. +-dependent purine nucleoside transporter from human kidney: cloning and functional characterization. Am. J. Physiol. 1997, 273 (6 Pt 2), F1058-F1065.
15. + / nucleoside cotransporter from pig kidney, pkCNT1. Biochim. Biophys. Acta 1998, 1415 (1), 266-269.
16. Wang, J.; Giacomini, K.M. Molecular determinants of substrate selectivity in Na + -dependent nucleoside transporters. J. Biol. Chem. 1997, 272 (46), 28845-28848.
17. Crawford, C.R.; Cass, C.E.; Young, J.D.; Belt, J.A. Stable expression of a recombinant sodium-dependent, pyrimidine-selective nucleoside transporter (CNT1) in a transport-deficient mouse leukemia cell line. Biochem. Cell. Biol. 1998, 76 (5), 843-851.
18. Fang, X.; Parkinson, F.E.; Mowles, D.A.; Young, J.D.; Cass, C.E. Functional characterization of a recombinant sodium-dependent nucleoside transporter with selectivity for pyrimidine nucleosides (cNT1rat) by transient expression in cultured mammalian cells. Biochem. J. 1996, 317 (Pt 2), 457-465.
19. Vickers, M.F.; Mani, R.S.; Sundaram, M.; Hogue, D.L.; Young, J.D.; Baldwin, S.A.; Cass, C.E. Functional production and reconstitution of the human equilibrative nucleoside transporter (hENT1) in Saccharomyces cerevisiae. Interaction of inhibitors of nucleoside transport with recombinant hENT1 and a glycosylation-defective derivative (hENT1/N48Q). Biochem. J. 1999, 339 (Pt 1), 21-32.
20. Vickers, M.F.; Yao, S.Y.; Baldwin, S.A.; Young, J.D.; Cass, C.E. Nucleoside transporter proteins of Saccharomyces cerevisiae. Demonstration of a transporter (FUI1) with high uridine selectivity in plasma membranes and a transporter (FUN26) with broad nucleoside selectivity in intracellular membranes. J. Biol. Chem. 2000, 275 (34), 25931-25938.
21. Vickers, M.F.; Young, J.D.; Baldwin, S.A.; Ellison, M.J.; Cass, C.E. Functional production of mammalian concentrative nucleoside transporters in Saccharomyces cerevisiae. Mol. Membr. Biol. 2001, 18 (1), 73-79.
22. Vickers, M.F.; Kumar, R.; Visser, F.; Zhang, J.; Charania, J.; Rabom, R.T.; Baldwin, S.A.; Young, J.D.; Cass, C.E. Comparison of the interaction of uridine, cytidine, and other pyrimidine nucleoside analogues with recombinant human equilibrative nucleoside transporter 2 (hENT2) produced in Saccharomyces cerevisiae. Biochem. Cell. Biol. 2002, 80 (5), 639-644.
23. Cheng, Y.; Prusoff, W.H. Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. Biochem. Pharmacol. 1973, 22 (23), 3099-3108.
24. Ito, H.; Fukuda, Y.; Murata, K.; Kimura, A. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 1983, 153 (1), 163-168.
25. Al-Salabi, M.I.; Wallace, L.J.; De Koning, H.P. A leishmania major nucleobase transporter responsible for allopurinol uptake is a functional homolog of the trypanosoma brucei H2 transporter. Mol. Pharmacol. 2003, 63 (4), 814-820.
26. Wallace, L.J.; Candlish, D.; De Koning, H.P. Different substrate recognition motifs of human and trypanosome nucleobase transporters. Selective uptake of purine antimetabolites. J. Biol. Chem. 2002, 277 (29), 26149-26156.
27. Zhang, J.; Visser, F.; Vickers, M.F.; Lang, T.; Robins, M.J.; Nielsen, L.P.C.; Nowak, I.; Baldwin, S.A.; Young, J.D.; Cass, C.E. Uridine binding motifs of human concentrative nucleoside transporters 1 and 3 (hCNT1 and hCNT3) produced in Saccharomyces cerevisiae. Mol. Pharmacol. 2003, 64 (6), in press.
28. Boleti, H.; Coe, I.R.; Baldwin, S.A.; Young, J.D.; Cass, C.E. Molecular identification of the equilibrative NBMPR-sensitive (es) nucleoside transporter and demonstration of an equilibrative NBMPR-insensitive (ei) transport activity in human erythroleukemia (K562) cells. Neuropharmacology 1997, 36 (9), 1167-1179.
29. Lum, P.Y.; Ngo, L.Y.; Bakken, A.H.; Unadkat, J.D. Human intestinal es nucleoside transporter: molecular characterization and nucleoside inhibitory profiles. Cancer Chemother. Pharmacol. 2000, 45 (4), 273-278.
30. Yao, S.Y.; Ng, A.M.; Sundaram, M.; Cass, C.E.; Baldwin, S.A.; Young, J.D. Transport of antiviral 3′(-deoxy-nucleoside drugs by recombinant human and rat equilibrative, nitrobenzylthioinosine (NBMPR)-insensitive (ENT2) nucleoside transporter proteins produced in Xenopus oocytes. Mol. Membr. Biol. 2001, 18 (2), 161-167.