On-line electrochemical tagging of free cysteines during nanospray ionisation for mass spectrometry analysis

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 5, Issue 32, 2004, Pages 30-37

  Roussel, Christophe ^a   Dayon, Loïc ^a   Rohner, Tatiana C ^a,b   Lion, Niels ^a   Girault, Hubert H ^a  

^a EPFL   (Switzerland)

^b NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Cyclic voltammetry; Electrochemical labelling; Mass fingerprint; Mass spectrometry; Microchip; Protein identification; Proteomics

Indexed keywords

CYCLIC VOLTAMMETRY; DNA; GENES; IONIZATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MICROORGANISMS; PROTEINS; REACTION KINETICS;

ELECTROCHEMICAL LABELLING; MASS FINGERPRINT; MICROCHIPS; PROTEIN IDENTIFICATION; PROTEOMICS;

ELECTROCHEMISTRY;

EID: 12144284384     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.554259     Document Type: Conference Paper

References (10)

1. D. Figeys "Proteomics in 2002: A year of technical development and wide-ranging applications" Anal. Chem., 75, 2891-2905, 2003.
2. D. F. Hochstrasser, J. C. Sanchez, R. D. Appel "Proteomics and its trends facing nature's complexity" Proteomics, 2, 807-812, 2002.
3. S. D. Patterson, R. H. Aebersold "Proteomics: The first decade and beyond" Nature Genet., 33, 311-323, 2003.
4. S. Sechi, B. T. Chait "Modification of cysteine residues by alkylation. A tool in peptide mapping and protein identification" Anal. Chem., 70, 5150-5158, 1998.
5. F. E. Regnier, L. Riggs, R. J. Zhang, L. Xiong, P. R. Liu, A. Chakraborty, E. Seeley, C. Sioma, R A. Thompson "Comparative proteomics based on stable isotope labeling and affinity selection" J. Mass Spectrom., 37, 133-145, 2002.
6. T. C. Rohner, J. S. Rossier, H. H. Girault "On-line electrochemical tagging of cysteines during nanospray" Electrochem. Commun., 4, 695-700, 2002.
7. C. Roussel, T. C. Rohner, H. Jensen, H. H. Girault "Mechanistic aspects of on-line electrochemical tagging of free L-cysteine residues during electrospray ionisation for mass spectrometry in protein analysis" ChemPhysChem, 4, 200-206, 2003.
8. C. Roussel, L. Dayon, H. Jensen, H. H. Girault "On-line cysteine modification for protein analysis: New probes for electrochemical tagging nanospray mass spectrometry" Journal of Electroanalytical Chemistry, 2004 in press.
9. L. Dayon, C. Roussel, H. H. Girault "On-line electrochemical tagging of free cysteines in peptides during nanospray ionisation mass spectrometry: an overview" Chimia, 4, 2004 in press.
10. L. Dayon, C. Roussel, M. Prudent, N. Lion, H. H. Girault "On-line quantification of cysteine residues in peptides during electrospray ionisation by electrogenerated tags and its application to proteins identification" 2004, submited for publication.