Overexpression and purification of reverse transcriptase of retron EC83 by changing the downstream sequence of the initiation codon

Journal of Microbiology and Biotechnology

Volumn 14, Issue 6, 2004, Pages 1280-1285

  Jeong, Dae Won ^a   Lim, Dongbin ^b  

^a Seoul National University   (South Korea)

^b Soongsil University   (South Korea)

Author keywords

Overexpression; Retron; Reverse transcriptase; Translational control

Indexed keywords

AMINO ACID; NUCLEOTIDE; RNA; RNA DIRECTED DNA POLYMERASE; SINGLE STRANDED DNA;

ARTICLE; CONTROLLED STUDY; DNA SYNTHESIS; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME PURIFICATION; ENZYME SYNTHESIS; GENE ISOLATION; GENE OVEREXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PROKARYOTE; PROMOTER REGION; RANDOMIZATION; RNA TRANSLATION; SEQUENCE ANALYSIS; START CODON; TRANSLATION INITIATION;

PROKARYOTA;

EID: 12144279501     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Etchegaray, J. P. and M. Inouye. 1999. Translational enhancement by an element downstream of the initiation codon in Escherichia coli. J. Biol. Chem. 274: 10079-10085.
2. Etchegaray, J. P. and M. Inouye. 1999. A sequence downstream of the initiation codon is essential for cold shock induction of cspB of Escherichia coli. J. Bacteriol. 181: 5852-5854.
3. Furuichi, T., S. Inouye, and M. Inouye. 1987. Biosynthesis and structure of stable branched RNA covalently linked to the 5′ end of multicopy single-stranded DNA of Stigmatella aurantiaca. Cell 48: 55-62.
4. Jeong, D. W., K. Kim, and D. Lim. 1997. Evidence for the complex formation between reverse transcriptase and multicopy single-stranded DNA in retron EC83. Mol. Cells 7: 347-351.
5. Kim, K., D. W. Jeong, and D. Lim. 1997. A mutational study of the site-specific cleavage of EC83, a multicopy single-stranded DNA (msDNA): Nucleotides at the msDNA stem are important for its cleavage. J. Bacteriol. 179: 6518-6521.
6. Kim, M. D., K.-N. Kim, and Y. J. Choi. 2003. Enhanced expression of β-xylosidase of Bacillus stearothermophilus No. 236 by change of translational inhibition codon in Escherichia coli and Bacillus subtilis. J. Microbiol. Biotechnol. 13: 584-590.
7. Kim, J. S., H. Y. Seong, M. W. Kim, J. S. Ku, and S. Y. Choi. 2003. Effects of minor arginyl tRNA and isoleucyl tRNA on the expression of Clostridium botulinum neurotoxin light chain in Escherichia coli. J. Microbiol. Biotechnol. 13: 287-291.
8. Kuroda, H. and P. Maliga. 2001. Sequences downstream of the translation initiation codon are important determinants of translation efficiency in chloroplasts. Plant Physiol. 125: 430-436.
9. Lee, J.-H., Y.-D. Cho, J. J. Choi, Y.-J. Lee, H.-S. Hoe, H.-K. Kim, and S.-T. Kwon. 2003. High-level expression in Escherichia coli of alkaline phosphatase from Thermus caldophilus GK24 and purification of the recombinant enzyme. J. Microbiol. Biotechnol. 13: 660-665.
10. Lim, D. 1991. Structure of two retrons of Escherichia coli and their common chromosomal insertion site. Mol. Microbiol. 5: 1863-1872.
11. Lim, D. 1992. Structure and biosynthesis of unbranched multicopy single-stranded DNA by reverse transcriptase in a clinical Escherichia coli isolate. Mol. Microbiol. 6: 3531-3542.
12. Lim, D., T. A. Gomes, and W. K. Maas. 1990. Distribution of msDNAs among serotypes of enteropathogenic Escherichia coli strains. Mol. Microbiol. 4: 1711-1714.
13. Lim, D. and W. K. Maas. 1989. Reverse transcriptase-dependent synthesis of a covalently linked, branched DNA-RNA compound in E. coli B. Cell 56: 891-904.
14. Lim, D. and W. K. Maas. 1989. Reverse transcriptase in bacteria. Mol. Microbiol. 3: 1141-1144.
15. Maas, W. K., C. Wang, T. Lima, G. Zubay, and D. Lim. 1994. Multicopy single-stranded DNAs with mismatched base pairs are mutagenic in Escherichia coli. Mol. Microbiol. 14: 437-441.
16. Oh, Y. P., S. T. Jeong, D.-W. Kim, E.-C. Kim, and K.-H. Yoon. 2002. Simple purification of shiga toxin B chain from recombinant Escherichia coli. J. Microbiol. Biotechnol. 12: 986-988.
17. Oh, K.-S., Y.-S. Park, and H.-C. Sung. 2002. Expression and purification of an ACE-inhibitory peptide multimer from synthetic DNA in Escherichia coli. J. Microbiol. Biotechnol. 12: 59-64.
18. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. 2nd Ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY. U.S.A.
19. Sorensen, M. A., C. G. Kurland, and S. Pedersen. 1989. Codon usage determines translation rate in Escherichia coli. J. Mol. Biol. 207: 365-377.
20. Sprengart, M. L., E. Fuchs, and A. G. Porter. 1996. The downstream box: An efficient and independent translation initiation signal in Escherichia coli. EMBO J. 15: 665-674.
21. Temin, H. M. 1989. Retrons in bacteria. Nature 339: 254-255.