Effects of minor arginyl tRNA and isoleucyl tRNA on the expression of clostridium botulinum neurotoxin light chain in Escherichia coli

Journal of Microbiology and Biotechnology

Volumn 13, Issue 2, 2003, Pages 287-291

  Kim, Jin Sook ^a   Seong, Hye Young ^a   Kim, Mi Wha ^a   Ku, Jong Seo ^a   Choi, Soon Yong ^a  

^a Hannam University   (South Korea)

Author keywords

Clostridium botulinum; Codon brias; Neurotoxin; tRNA

Indexed keywords

ARGININE TRANSFER RNA; BOTULINUM TOXIN A; DNA; ISOLEUCINE TRANSFER RNA; NEUROTOXIN; PROTEINASE; RECOMBINANT PROTEIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; ZINC;

ANIMAL CELL; ARTICLE; BACTERIAL GENE; BACTERIOPHAGE T4; CLOSTRIDIUM BOTULINUM; CODON; CONTROLLED STUDY; DNA VECTOR; ESCHERICHIA COLI; EXOCYTOSIS; GENE EXPRESSION; GENE FREQUENCY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ANIMALIA; BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM BOTULINUM; ESCHERICHIA COLI; ILEX;

EID: 0038025787     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Binz, T., J. Blasi, S. Yamasaki, A. Baumeister, E. Link, T. C. Sudhof, R. Jahn, and H. Niemann. 1994. Proteolysis of SNAP-25 by Types E and A botulinal neurotoxins. J. Biol. Chem. 269: 1617-1620.
2. Binz, T., H. Kurazono, M. Wille, J. Frevert, K. Wernars, and H. Niemann. 1990. The complete sequence of botulinum neurotoxin type A and comparison with other Clostridial neurotoxins. J. Biol. Chem. 265: 9153-9158.
3. 125I-labeled botulinum neurotoxins with nerve terminals. I. Ultrastructural autoradiographic localization and quantitation of distinct membrane acceptors for types A and B on motor nerves. J. Cell Biol. 103: 521-534.
4. Garcia, G. M., P. K. Mar, D. A. Mullin, J. R. Walker, and N. E. Prather. 1986. The Escherichia coli dna Y gene encodes an arginine transfer RNA. Cell 45: 453-459.
5. Glick, B. R. and J. J. Pasternak. 1998. Molecular Biotechnology, 2nd Ed. p. 34. ASM Press, Washington, D.C., U.S.A.
6. Hatheway, C. L. 1990. Toxigenic clostridia. Clin. Microbiol. Rev. 3: 66-98.
7. Henderson, I., T. Davis, M. Elmore, and N. P. Minton. 1997. The genetic basis of toxin production in Clostridium botulinum and Clostridium tetani, pp. 261-294. In Rood, J. L., McClane, B. A., Songer, J. G. and Titball, R. W. (eds.). The Clostridia: Molecular Biology and Pathogenesis. Academic Press, CA, U.S.A.
8. Kane, J. F. 1995. Effects of rare codon cluster on high-level expression of heterologous proteins in Escherichia coli. Curr. Opin. Biotechnol. 6: 494-500.
9. Kim, K. S., I. S. Lee, S. Lee, Y. P. Lee, C. Jung, H. C. Kim, and S. Y. Choi. 2002. Cloning and expression of the aminopeptidase gene from the Bacillus licheniformis in Bacillus subtilis. J. Microbiol. Biotechnol. 12: 773-779.
10. Kurland, C. and J. Gallant. 1996. Errors of heterologous protein expression. Curr. Opin. Biotechnol. 7: 481-493.
11. Li, L. and B. R. Singh. 1999. High-level expression, purification, and characterization of recombinant type A botulinum neurotoxin light chain. Protein Expres. Purif. 17: 339-344.
12. Mazzara, G. P., G. Plunkett, III. and W. H. McClain. 1981. DNA sequence of the transfer RNA region of bacteriophage T4: Implications for transfer RNA synthesis. Proc. Natl. Acad. Sci. USA 78: 889-892.
13. Muramatsu, T., K. Nidhikawa, F. Nemoto, Y. Kuchino, S. Nishimura, T. Miyazawa, and S. Yokoyama. 1988. Codon and amino-acid specificities of a transfer RNA are both converted by a single post-transcriptional modification. Nature 336: 179-181.
14. Oh, K. S., Y. S. Park, and H. C. Sung. 2002. Expression and purification of an ACE-inhibitory peptide multimer from synthetic DNA in Escherichia coli. J. Microbiol. Biotechnol. 12: 59-64.
15. Park, K. S., S. W. Park, and S. Y. Choi. 2000. Improved T-vector for the cloning of PCR DNA using green fluorescent protein. J. Microbiol. Biotechnol. 10: 264-266.
16. Schiavo, G., O. Rossetto, S. Catsicas, P. Polverino de Laureto, B. R. DasGupta, F. Benfenati, and C. Montecucco. 1993. Identification of the nerve tenninal targets of botulinum neurotoxin serotypes A, D, and E. J. Biol. Chem. 268: 23784-23787.
17. Schmidt, J. J. and K. A. Bostian. 1995. Proteolysis of synthetic peptides by type A botulinum neurotoxin. J. Protein Chem. 14: 703-708.
18. Simpson, L. L. 1986. Molecular pharmacology of botulinum toxin and tetanus toxin. Annu. Rev. Pharmacol. Toxicol. 26: 427-453.
19. Simpson, L. L. and B. R. DasGupta. 1983. Botulinum neurotoxin type E: Studies on mechanism of action and on structure activity relationships. J. Pharmacol. Exp. Ther. 224: 135-140.
20. leu overproduction in Escherichia coli. Mol. Microbiol. 7: 253-263.
21. Zhang, S., G. Zubay, and E. Goldman. 1991. Low-usage codons in Escherichia coli, yeast, fruit fly and primates. Gene 105: 61-72.