Optical tomography of pigmented human skin biopsies

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 5, Issue 1, 2004, Pages 24-34

  Riemann, Iris ^a   Fischer, Peter ^b   Kaatz, Martin ^c   Fischer, Tobias W ^c   Eisner, Peter ^c   Dimitrov, Enrico ^c   Reif, Annette ^c   Konig K ^a,d  

^a FRAUNHOFER INSTITUTE FOR BIOMEDICAL ENGINEERING   (Germany)

^b JENLAB GMBH   (Germany)

^c FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^d IBMT ^*  (Germany)

Author keywords

Autofluorescence; Femtosecond laser; Fluorescence lifetime; Melanin; Multiphoton; Optical tomography; Skin

Indexed keywords

AUTOFLUORESCENCE; FEMTOSECOND LASER; FLUORESCENCE LIFETIME; MELANIN; MULTIPHOTON TECHNIQUE; OPTICAL TOMOGRAPHY;

BIOPSY; FLUORESCENCE; INFRARED RADIATION; LASER BEAM EFFECTS; LASER PULSES; LUMINESCENCE; PHOTONS; PIGMENTS; PROTEINS; TISSUE; TOMOGRAPHY; TUMORS;

SKIN;

EID: 12144261626     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.528278     Document Type: Conference Paper

References (18)

1. Handbook of Biological Confocal Microscopy, J. B. Pawley, Ed., Plenum, New York (1995)
2. M. Rajadhyaksha, S. Gonzales, J. M. Zavislan, R. R. Anderson, and R. H. Webb, " In vivo confocal laser scanning microscopy of human skin II: advances in instrumentation and comparison with histology", J. Invest. Dermatol. 113, 293-303 (1999)
3. M. Rajadhyaksha, R. R. Anderson, and R. H. Webb, "Video-rate confocal scanning laser microscope for imaging human tissues in vivo", Appl. Opt. 38, 2105-2115 (1999)
4. B. R. Masters, "Three-dimensional confocal microscopy of human skin in vivo: autofluorescence of human skin", Bioimaging 4, 13-19 (1996)
5. K. König, U. Wollina, I. Riemann, C. Peuckert, K. J. Halbhuber, H. Konrad, P. Fischer, V. Fünfstück, T.W. Fischer, and P. Elsner, "Optical tomography of human skin with subcellular spatial and picosecond time resolution using near infrared-femtosecond laser pulses ", Proc. SPIE 4620, 190-201 (2002)
6. M. Göppert-Mayer, "Über Elementarakte mit zwei Quantensprüngen", Göttinger Dissertaion, Ann Phys 9, 273-294 (1931)
7. W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning microscopy", Science (Washington DC, U.S.) 248, 73-76 (1990)
8. K. König, "Review: multiphoton microscopy in life sciences", J. Microsc. 200, 83-104 (2000)
9. X. Deng, X. Gan, and M. Gu, "Multiphoton fluorescence microscopic imaging through double-layer turbid tissue media", J. Appl. Phys. 91, 4659-4665 (2002)
10. B. R. Masters, P. T. C. So, and E. Gratton, "Multiphoton excitation microscopy of in vivo human skin", Ann. N.Y. Acad. Sci. 838, 58-67 (1998)
11. P. T. C. So and H. Kim, "Two-photon deep tissue ex vivo imaging of mouse dermal and subcutaneous structures", Opt. Express 3, 339-350 (1998)
12. R. F. M. Hendriks and G. W. Lucassen, "Two photon fluorescence microscopy of in vivo human skin", Proc. SPIE 4164 (1999)
13. K. König, "Laser tweezers and multiphoton microscopes in life sciences", Histochem. Cell. Biol. 144, 79-92 (2000)
14. K. König, C. Peuckert, I. Riemann, and U. Wollina, " Non-invasive 3D optical biopsy of human skin with NIR-femtosecond laser pulses for diagnosis of dermatological disorders", Cell. Mol. Biol. 46, abstract 117 (2000)
15. K. König and I. Riemann,"High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution", J. Biomed. Optics 8, 432-439 (2003)
16. K. Teuchner, W. Freyer, D. Leupold, A. Volkmer, D. J. Birch, P. Altmeyer, M. Strucker, and K. Hoffman,"Femtosecond two-photon excited fluorescence of melanin", Photochem. Photobiol. 70, 146-151 (1999)
17. K. Teuchner, J. Ehlert, W. Freyer, D. Leupold, P. Altmeyer, M. Stücker, and k. Hoffmann, "Fluorescence studies of melanin by stepwise two-photon femtosecon laser excitation", J. Fluorescence 10, 275-281 (2000)
18. W. Becker, A. Bergmann, K. König, and U. Tirlapur, "Picosecond fluorescence lifetime microscopy by TCSPC imaging", Proc. SPIE 4262, 414-419 (2001)